Galactose Oxidase/Prussian Blue Based Biosensors

Charmantray, Franck; Touisni, Nadia; Hecquet, Laurence; Noguer, Thierry; Mousty, Christine

doi:10.1002/elan.201400720

Cited by 7 publications

(10 citation statements)

References 10 publications

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“…This buffer is also suitable for the electrochemical detection of the activity of TK, and KCl is necessary for the electrochemical reduction of Prussian blue [Eqs. (1) and (2)] . Under these conditions, the oxidation peak attributed to the oxidation of Prussian white (PW) into Prussian blue is observed at +0.2 V vs. Ag/AgCl, and the reverse reaction is found at a potential of +0.04 V vs. Ag/AgCl, in accordance with the literature .…”

Section: Resultsmentioning

confidence: 99%

“…Similar experiments conducted with l ‐ery led to an optimal GAOx quantity of 0.245 μg (3.6 pmol) (Figure S2). In previous work, a similar amperometric biosensor developed on a glassy carbon electrode required nearly 700 times more GAOx (immobilized in laponite and reticulated by glutaraldehyde), which shows that the present configuration is more efficient …”

Section: Resultsmentioning

confidence: 99%

“…Previously, GAOx was immobilized in gelatin, conducting poly( N ‐glycidylpyrrole‐co‐pyrrole) films, and oxirane acrylic beads (Eupergit C) for biosensor design applications. More recently, a colloidal suspension of both GAOx and laponite was adsorbed and reticulated with glutaraldehyde at the surface of platinum or Prussian blue screen‐printed electrodes . Here, four immobilization conditions were selected as a case study: GAOx adsorbed without matrix at the surface of the electrodes (GAOx ads), GAOx coadsorbed in the presence of laponite (GAOx@L), GAOx coadsorbed in the presence of alginate (GAOx@alg), a negatively charged biopolymer, and GAOx coadsorbed in the presence of both components (GAOx@ l ‐Alg).…”

Section: Resultsmentioning

confidence: 99%

“…Most of these biosensors are based on the electrochemical oxidation of H 2 O 2 at the electrode surface or on the reduction of an electrochemical mediator such as ferrocene or Prussian blue . To our knowledge, no GAOx‐based biosensor using (electro)chemiluminescence as a detection system has ever been described.…”

Section: Introductionmentioning

confidence: 99%

“…[10] GAOx has been used since the 1990s in electrochemical biosensors for the detection of galactose, [11,12] lactose (together with a b-galactosidase), [13] dihydroxyacetone (DHA), [14][15][16] and, to al esser extent, superoxide radicalsa nd histidine (in the presence of ascorbic acid). [17,18] More recently,i tw as used for the detection of transketolase( TK) activity,a ne nzyme involved in different pathologies: [19,20] transketolase reactionp roducts can be specifically oxidized by GAOx;t his leads to the coproductiono fH 2 O 2 ,w hich is detected by amperometry.…”

Section: Introductionmentioning

confidence: 99%

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High‐Throughput Electrochemical Screening Assay for Free and Immobilized Oxidases: Electrochemiluminescence and Intermittent Pulse Amperometry

Aymard

Bonaventura²,

Henkens³

et al. 2016

ChemElectroChem

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Analysis of a large number of samples by electrochemical methods for rapid optimization of amperometric biosensors or screening applications is still a challenge. To overcome this limitation, a system with 96 screen‐printed electrodes was developed to assay H2O2 produced by free and immobilized galactose oxidase as a model for oxidases. Detection was based on two electrochemical methods: intermittent pulse amperometry (IPA) and electrochemiluminescence (ECL). This 96‐well electrochemical device allowed rapid optimization of the parameters in half a day, regardless of the method used (i.e., IPA or ECL). We demonstrate that ECL is more sensitive for the detection of H2O2 (limit of detection of 1 μm for ECL vs. 10 μm for IPA) with lower interelectrode variability (1 vs. 19 %). Rapid determination of sensitivities, linear ranges, and limits of detection of galactose oxidase substrates (e.g., dihydroxyacetone and l‐erythrulose) by using free or immobilized enzyme was conducted in only 50 min by the mean of 5 different sets of 96 screen‐printed electrodes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High‐Throughput Electrochemical Screening Assay for Free and Immobilized Oxidases: Electrochemiluminescence and Intermittent Pulse Amperometry

Aymard

Bonaventura²,

Henkens³

et al. 2016

ChemElectroChem

Self Cite

View full text Add to dashboard Cite

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Hydrothermal Recrystallization as a Strategy to Reveal the Structural Diversity in Hexacyanometallates: Nickel and Copper Hexacyanoosmates(II)

et al. 2020

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The results discussed herein reinforce the relevance of the hydrothermal recrystallization in transition metal hexacyanometallates as a suitable strategy to access the structural diversity of this family of coordination polymers. Copper(2+) and nickel(2+) hexacyanoosmates(II) were prepared by the precipitation method using hexacyanoosmic(II) acid as the complex anion source, obtaining powders of insufficient crystalline quality for the structural study. These powders were then recrystallized under hydrothermal conditions. For Ni, three new phases where formed, monoclinic and tetragonal, and a hexagonal modification when the monoclinic one is dehydrated under soft heating. For copper, one new tetragonal phase was [a] Prof. 1763found, in addition to the previously reported cubic one, typical of Prussian blue analogues. The crystal structure for these four nickel and cooper hexacyanoosmates(II) were solved and refined ab initio from powder XRD data. The refined crystal structures are properly supported by a detailed spectroscopic study from IR and UV/Vis data, Ligand Field Analysis, and thermal, magnetic and hydrogen adsorption measurements. In the anhydrous phase for nickel, neighboring Ni atoms were found coordinated by bifurcated N end, an atypical coordination mode for the CNligand. The results herein discussed contribute to shed light on the vast information unrevealed on the coordination chemistry of transition metal hexacyanometallates. catalysis, [6] gas storage and separation, [7] batteries, [8] and environmental remediation, [9] etc. However, it is not possible to divorce both topics since functionality directly depends on the material's electronic and crystalline structure.The most studied polymeric hexacyanometallates are those where the internal metal center (M, linked to the C end of the CNgroup) belongs to the first transition series. This obeys to the greater availability of the building blocks, to historical reasons, but also to the fact that they are obtained with good crystallinity by the very easy precipitation method. The hexacyanide anions containing metals with relatively diffuse d orbitals -corresponding to the second and third transition series -have proved to improve the robustness of the polymeric frameworks. [10] However, when these compounds are prepared by the traditional precipitation method, they frequently exhibit relatively low crystallinity. [11] This is a critical issue for the structures containing a great number of vacancies of the hexacyanide building block around the outer metal (T, linked to the N end of the CNgroup), which requires recrystallization procedures after precipitation of the crude compound. [10] In this work, we explore the potentialities of solvothermal recrystallization, beyond improving the crystallinity of materials, with the aim of generating structural diversity.In general, structural diversity for each combination of inner and outer metals, is not frequently observed in polymeric hexacyanometallates. A unique behavior in this sense appears for Co 2+ a...

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Paper‐based Potentiometric Biosensor for Monitoring Galactose in Whole Blood

et al. 2020

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A filter paper sputtered with a layer of Pt and subsequently coated with a Nafion® membrane is used as working electrode. The mixed potential of the Pt electrode allows the detection of H2O2 generated by the oxidation of galactose in the presence of the galactose oxidase enzyme. This provides a simple and mediator‐free approach method. The system shows sensitivity values of −62.8±9.4 mV/decade of galactose in the range from 0.3 to 31.6 mM, well within the clinical relevant range. MnO2 nanoparticles were added to decrease the interference from ascorbic acid so that validation of the sensor in whole blood samples was performed with good recovery.

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Galactose Oxidase/Prussian Blue Based Biosensors

Cited by 7 publications

References 10 publications

High‐Throughput Electrochemical Screening Assay for Free and Immobilized Oxidases: Electrochemiluminescence and Intermittent Pulse Amperometry

High‐Throughput Electrochemical Screening Assay for Free and Immobilized Oxidases: Electrochemiluminescence and Intermittent Pulse Amperometry

Hydrothermal Recrystallization as a Strategy to Reveal the Structural Diversity in Hexacyanometallates: Nickel and Copper Hexacyanoosmates(II)

Paper‐based Potentiometric Biosensor for Monitoring Galactose in Whole Blood

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