Galactose-Induced Skin Aging: The Role of Oxidative Stress

Umbayev, Bauyrzhan; Askarova, Sholpan; Almabayeva, Aigul; Saliev, Timur; Masoud, Abdul-Razak; Bulanin, Denis

doi:10.1155/2020/7145656

Cited by 67 publications

(64 citation statements)

References 150 publications

(253 reference statements)

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“…These results are similar to what previously reported by our group on human erythrocytes exposed in vitro to H 2 O 2 [ 27 ]. Recent scientific evidences have reported that d- Gal accumulation induces oxidative stress, cell senescence and cytotoxicity [ 61 ]. In this regard, B3p functions, being crucial in the systemic homeostasis maintenance and affected by oxidative stress, could be correlated to oxidative stress-induced alterations during galactosemia.…”

Section: Discussionmentioning

confidence: 99%

d-Galactose Decreases Anion Exchange Capability through Band 3 Protein in Human Erythrocytes

et al. 2020

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d-Galactose (d-Gal), when abnormally accumulated in the plasma, results in oxidative stress production, and may alter the homeostasis of erythrocytes, which are particularly exposed to oxidants driven by the blood stream. In the present investigation, the effect of d-Gal (0.1 and 10 mM, for 3 and 24 h incubation), known to induce oxidative stress, has been assayed on human erythrocytes by determining the rate constant of SO42− uptake through the anion exchanger Band 3 protein (B3p), essential to erythrocytes homeostasis. Moreover, lipid peroxidation, membrane sulfhydryl groups oxidation, glycated hemoglobin (% A1c), methemoglobin levels (% MetHb), and expression levels of B3p have been verified. Our results show that d-Gal reduces anion exchange capability of B3p, involving neither lipid peroxidation, nor oxidation of sulfhydryl membrane groups, nor MetHb formation, nor altered expression levels of B3p. d-Gal-induced %A1c, known to crosslink with B3p, could be responsible for rate of anion exchange alteration. The present findings confirm that erythrocytes are a suitable model to study the impact of high sugar concentrations on cell homeostasis; show the first in vitro effect of d-Gal on B3p, contributing to the understanding of mechanisms underlying an in vitro model of aging; demonstrate that the first impact of d-Gal on B3p is mediated by early Hb glycation, rather than by oxidative stress, which may be involved on a later stage, possibly adding more knowledge about the consequences of d-Gal accumulation.

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Section: Discussionmentioning

confidence: 99%

d-Galactose Decreases Anion Exchange Capability through Band 3 Protein in Human Erythrocytes

et al. 2020

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“…Galactose is a physiological nutrient that is chemically a reducing carbohydrate. It can be combining with glucose and form the disaccharide lactose [50,51]. Xylose or wood sugar has antifungal and antibacterial properties.…”

Section: Resultsmentioning

confidence: 99%

Determination of Carbohydrates Content in Gentiana Cruciata L. By Gc/MS Method

et al. 2021

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Objective: Thus, the aim of our research was to determine the qualitative composition and quantitative content of carbohydrates in the studied plant material with the prospect of its application as a medicinal plant raw material. Methods: The carbohydrates of the herb of Gentiana cruciata L. determined by GC/MS method. Identification of monosaccharides was based on comparing their retention times with retention times of standards of the mass spectral library NIST 02. Quantification was done by using sorbitol added to the sample. Results: The quantitative content of 4 free carbohydrates such as D-saccharose (38.39 mg/g), D-Pinitol (12.01 mg/g), D-glucose (10.05 mg/g) and D-fructose (1.69 mg/g) was established in the herb of Gentiana cruciata L. Also, this method established the qualitative composition and quantitative content of eight carbohydrates (monosaccharides and their derivatives after hydrolysis): D-glucose (29.66 mg/g), D-Pinitol (22.24 mg/g), L-arabinose (4.26 mg/g), D-galactose (3.55 mg/g), D-xylose (1.80 mg/g), L-rhamnose (1.49 mg/g), D-Dulcitol (0.76 mg/g) and D-mannose (0.44 mg/g). Conclusion: The results of the study showed that carbohydrates from the Gentiana cruciata L. can be used as important resources of new ingredients for the pharmaceutical industry.

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“…Enzyme deficiency in this pathway results in a disease called galactosemia [ 54 , 55 , 56 ]. Previous studies in humans showed that phosphorus in the RBCs and plasma may be decreased due to accumulation of gal-1-P in the cells [ 57 , 58 , 59 , 60 ]. In the D-gal model, a high level of galactose phosphorylation may be the cause of a decrease in the phosphorus fraction.…”

Section: Discussionmentioning

confidence: 99%

Spectroscopic Signature of Red Blood Cells in a D-Galactose-Induced Accelerated Aging Model

Blat

Stepanenko

Bulat

et al. 2021

IJMS

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This work presents a semi-quantitative spectroscopic approach, including FTIR–ATR and Raman spectroscopies, for the biochemical analysis of red blood cells (RBCs) supported by the biochemical, morphological and rheological reference techniques. This multi-modal approach provided the description of the RBC alterations at the molecular level in a model of accelerated aging induced by administration of D-galactose (D-gal), in comparison to natural aging. Such an approach allowed to conclude that most age-related biochemical RBC membrane changes (a decrease in lipid unsaturation and the level of phospholipids, or an increase in acyl chain shortening) as well as alterations in the morphological parameters and RBC deformability are well reflected in the D-gal model of accelerated aging. Similarly, as in natural aging, a decrease in LDL level in blood plasma and no changes in the fraction of glucose, creatinine, total cholesterol, HDL, iron, or triglycerides were observed during the course of accelerated aging. Contrary to natural aging, the D-gal model led to an increase in cholesterol esters and the fraction of total esterified lipids in RBC membranes, and evoked significant changes in the secondary structure of the membrane proteins. Moreover, a significant decrease in the phosphorous level of blood plasma was specific for the D-gal model. On the other hand, natural aging induced stronger changes in the secondary structures of the proteins of the RBCs’ interior. This work proves that research on the aging mechanism, especially in circulation-related diseases, should employ the D-gal model with caution. Nonetheless, the D-gal model enables to imitate age-related rheological alterations in RBCs, although they are partially derived from different changes observed in the RBC membrane at the molecular level.

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Galactose-Induced Skin Aging: The Role of Oxidative Stress

Cited by 67 publications

References 150 publications

d-Galactose Decreases Anion Exchange Capability through Band 3 Protein in Human Erythrocytes

d-Galactose Decreases Anion Exchange Capability through Band 3 Protein in Human Erythrocytes

Determination of Carbohydrates Content in Gentiana Cruciata L. By Gc/MS Method

Spectroscopic Signature of Red Blood Cells in a D-Galactose-Induced Accelerated Aging Model

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