Galactomannan‐Alginate Synergism Applied in Albumin Encapsulation

Valenga, Francine; Lucyszyn, Neoli; Ono, Lucy; Souza, Camilla Mariane Menezes; Lubambo, Adriana F.; Sierakowski, Maria Rita

doi:10.1002/masy.200900096

Cited by 4 publications

(2 citation statements)

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“…Rheological properties of mixtures of polysaccharides have in the past mainly been studied for polysaccharides that interact specifically, with the aim to exploit the synergy resulting from these interactions for potential applications, see for example refs. − Also, segregative phase separation of incompatible polysaccharides has been studied in some depth, see for example refs − However, we are not aware of investigations of the viscosity of homogeneous mixtures of polysaccharides without specific interactions over a broad range of concentrations. Potentially one could use such mixtures to fine tune both η 0 at a given polysaccharide concentration and the shear rate dependence for a given η 0 .…”

Section: Introductionmentioning

confidence: 99%

Viscosity of Aqueous Polysaccharide Solutions and Selected Homogeneous Binary Mixtures

et al. 2020

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The viscosity (η) of dextran (Dex), methyl cellulose (MC), hydroxypropylmethyl cellulose (HPMC), κ-carrageenan (KC), alginate (Alg), and carboxymethyl cellulose (CMC) was measured as a function of the shear rate (γ) over a broad range of concentrations both in salt-free water and in 0.1 M NaCl. The concentration (C) dependence of the Newtonian viscosity (η 0 ) was found to be universal for neutral polysaccharides and anionic polysaccharides in 0.1 M NaCl when C was multiplied with the intrinsic viscosity. The dependence was compared with theoretical predictions assuming dominant hydrodynamic or dominant topological interactions. The effect of electrostatic interactions was observed for anionic polysaccharides in salt-free water but not at higher concentrations. Master curves of the shear thinning behavior at different concentrations were obtained when η/η 0 was plotted versus τ. γ, with τ being a concentration-dependent time that characterizes the onset of shear thinning. Binary mixtures of two neutral polysaccharides (MC and HPMC), two anionic polysaccharides (Alg and CMC), and a neutral (Dex) and anionic polysaccharide (KC) were investigated as a function of the composition and the total polysaccharide concentration. The behavior of the first two mixtures was similar to that of a single polysaccharide with an intermediate intrinsic viscosity. Significant synergy was observed for the third type of mixtures in salt-free water demonstrating the important effect of electrostatic interactions between charged polysaccharides at low concentrations even in concentrated solutions of neutral polysaccharides.

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Section: Introductionmentioning

confidence: 99%

Viscosity of Aqueous Polysaccharide Solutions and Selected Homogeneous Binary Mixtures

et al. 2020

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“…The purified galactomannan (GML), extracted from Leucaena leucocephala seeds, is composed of mannose:galactose (Man:Gal) at a ratio of 1.7, has an average molar mass of 713,600 g/mol, and carbohydrate and protein composition of 83% and 5% (w/w), respectively [24]. MnCl 2 and CaCl 2 were analytical grade (LabSynth, São Paulo, Brazil) and used without further purification ConA, type IV, from Concanavalia ensiformis with molar mass of 25.583 g/mol, was purchased from Sigma (C2010, St. Louis, USA) and used without any purification.…”

Section: Methodsmentioning

confidence: 99%

Galactomannan thin films as supports for the immobilization of Concanavalin A and/or dengue viruses

Valenga

Petri

Lucyszyn

et al. 2012

International Journal of Biological Macromolecules

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The immobilization of the glucose/mannose-binding lectin from Concanavalia ensiformis seeds (ConA) onto a monolayer made of a galactomannan extracted from Leucaena leucocephala seeds (GML), which was adsorbed onto - amino-terminated surfaces, was investigated by means of ellipsometry and atomic force microscopy. The mean thickness of GML monolayer, which polysaccharide consists of linear 1→4-linked β-D-mannopyranosil units partially substituted at C-6 by α-D-galactopyranosyl units, amounted to (1.5±0.2) nm. ConA molecules adsorbed onto GML surfaces forming (2.0±0.5) nm thick layers. However, in the presence of mannose the adsorption failed, indicating that ConA binding sites were blocked by mannose and were no longer available for mannose units present in the GML backbone. The GML film was also used as support for the adsorption of three serotypes of dengue virus particles (DENV-1, DENV-2 and DENV-3), where DENV-2 formed the thickest film (4±2) nm. The adsorbed layer of DENV-2 onto ConA-covered GML surfaces presented mean thickness values similar to that determined for DENV-2 onto bare GML surfaces. The addition of free mannose units prevented DENV-2 adsorption onto ConA-covered GML films by ~50%, suggesting competition between virus and mannose for ConA binding sites. This finding suggests that if ConA is also adsorbed to GML surface and its binding site is blocked by free mannose, virus particles are able to recognized GML mannose unities substituted by galactose. Interactions between polysaccharides thin films, proteins, and viruses are of great relevance since they can provide basis for the development of biotechnological devices. These results indicate that GML is a potential polysaccharide for biomaterials development, as those could involve interactions between ConA in immune system and viruses.

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Characterization of rheological interactions of Gleditsia triacanthos gum with some hydrocolloids: Effect of hydration temperature

2013

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Galactomannan‐Alginate Synergism Applied in Albumin Encapsulation

Cited by 4 publications

References 30 publications

Viscosity of Aqueous Polysaccharide Solutions and Selected Homogeneous Binary Mixtures

Viscosity of Aqueous Polysaccharide Solutions and Selected Homogeneous Binary Mixtures

Galactomannan thin films as supports for the immobilization of Concanavalin A and/or dengue viruses

Characterization of rheological interactions of Gleditsia triacanthos gum with some hydrocolloids: Effect of hydration temperature

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