Galacto-oligosaccharides Protect the Intestinal Barrier by Maintaining the Tight Junction Network and Modulating the Inflammatory Responses after a Challenge with the Mycotoxin Deoxynivalenol in Human Caco-2 Cell Monolayers and B6C3F1 Mice

Akbari, Peyman; Braber, Saskia; Alizadeh, Abolfath; Verheijden, Kim A.T.; Schoterman, Margriet H. C.; Kraneveld, Aletta D.; Garssen, Johan; Fink‐Gremmels, Johanna

doi:10.3945/jn.114.209486

Cited by 115 publications

(124 citation statements)

References 67 publications

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“…An in vitro study by Bhatia et al (Bhatia et al 2015) showed that GOS enhanced intestinal barrier function through modulation of the secretory function of human goblet cells by up regulation of related genes and protein expression (Bhatia et al 2015). GOS can also stimulate in vitro tight junction assembly in gut intestinal human Caco-2 cells, which is beneficial for the integrity of the epithelial barrier (Akbari et al 2015). Furthermore, GOS can prevent deoxynivalenol-induced histomorphologic alterations in the villi of the mouse intestine, indicating that it has protective effects on villi as well, which is important for undisturbed adsorption of nutrients (Akbari et al 2015).…”

Section: Effects Of Gos On Epithelial Cells Responsesmentioning

confidence: 99%

“…GOS can also stimulate in vitro tight junction assembly in gut intestinal human Caco-2 cells, which is beneficial for the integrity of the epithelial barrier (Akbari et al 2015). Furthermore, GOS can prevent deoxynivalenol-induced histomorphologic alterations in the villi of the mouse intestine, indicating that it has protective effects on villi as well, which is important for undisturbed adsorption of nutrients (Akbari et al 2015). In another study by Akbari et al (2017) it was found that GOS could beneficially influence barrier integrity of Caco-2 cells in vitro.…”

Section: Effects Of Gos On Epithelial Cells Responsesmentioning

confidence: 99%

“…However, since both GOS and FOS do have direct effects on epithelial cells (Akbari et al 2015(Akbari et al , 2017, we also expect mixtures of GOS and FOS to exert beneficial effect on epithelial barrier integrity.…”

Section: Effects Of Gos/fos Mixtures On Epithelial Cell Responsesmentioning

confidence: 99%

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Non-digestible carbohydrates in infant formula as substitution for human milk oligosaccharide functions: Effects on microbiota and gut maturation

Akkerman

Faas²,

Vos

2018

Critical Reviews in Food Science and Nutrition

131

140

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Human milk (HM) is the golden standard for nutrition of newborn infants. Human milk oligosaccharides (HMOs) are abundantly present in HM and exert multiple beneficial functions, such as support of colonization of the gut microbiota, reduction of pathogenic infections and support of immune development. HMO-composition is during lactation continuously adapted by the mother to accommodate the needs of the neonate. Unfortunately, for many valid reasons not all neonates can be fed with HM and are either totally or partly fed with cow-milk derived infant formulas, which do not contain HMOs. These cow-milk formulas are supplemented with non-digestible carbohydrates (NDCs) that have functional effects similar to that of some HMOs, since production of synthetic HMOs is challenging and still very expensive. However, NDCs cannot substitute all HMO functions. More efficacious NDCs may be developed and customized for specific groups of neonates such as pre-matures and allergy prone infants. Here current knowledge of HMO functions in the neonate in view of possible replacement of HMOs by NDCs in infant formulas is reviewed. Furthermore, methods to expedite identification of suitable NDCs and structure/function relationships are reviewed as in vivo studies in babies are impossible.

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Section: Effects Of Gos On Epithelial Cells Responsesmentioning

confidence: 99%

Section: Effects Of Gos On Epithelial Cells Responsesmentioning

confidence: 99%

See 1 more Smart Citation

Non-digestible carbohydrates in infant formula as substitution for human milk oligosaccharide functions: Effects on microbiota and gut maturation

Akkerman

Faas²,

Vos

2018

Critical Reviews in Food Science and Nutrition

131

140

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“…Male B6C3F 1 and male BALB/c mice (6-8 weeks old) obtained from Charles River (Maastricht, The Netherlands) were housed under controlled conditions in standard laboratory cages or biocontained sterile conditions using HEPA-filter Isocages® (Tecniplast, Buguggiate, Italy), respectively. These data were obtained from analyses of samples from recently published studies [6,12]. …”

Section: Methodsmentioning

confidence: 99%

“…Interestingly, a reduction in the incidence of allergic manifestations and infections was observed after nutritional application with prebiotic oligosaccharides (90% GOS/10% lcFOS) [4,5]. Recent work by our group demonstrated that GOS also have microbiota-independent properties observed in intestinal epithelial cells [6,7]. The homeostasis of the epithelial inflammatory response within the intestinal epithelium can be regulated by the axis of interleukin 33 (IL-33) and its receptor ST2, which belongs to the IL-1/Toll-like receptor (TLR) superfamily [8].…”

Section: Introductionmentioning

confidence: 99%

Inflammation-Induced Expression of the Alarmin Interleukin 33 Can Be Suppressed by Galacto-Oligosaccharides

Verheijden

Akbari²,

Willemsen³

et al. 2015

Int Arch Allergy Immunol

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Background: The alarmin interleukin 33 (IL-33) and its receptor ST2 play an important role in mucosal barrier tissues, and seem to be crucial for Th2-cell mediated host defense. Galacto-oligosaccharides (GOS), used in infant formulas, exhibit gut and immune modulatory effects. To enhance our understanding of the immunomodulatory capacity of GOS, this study investigated the impact of dietary GOS intervention on IL-33 and ST2 expression related to intestinal barrier dysfunction and asthma. Methods: B6C3F₁ and BALB/c mice were fed a control diet with or without 1% GOS. To simulate intestinal barrier dysfunction, B6C3F₁ mice received a gavage with the mycotoxin deoxynivalenol (DON). To mimic asthma-like inflammatory airway responses, BALB/c mice were sensitized on day 0 and challenged on days 7-11 with house-dust mite (HDM) allergen. Samples from the intestines and lungs were collected for IL-33 and ST2 analysis by qRT-PCR, immunoblotting and immunohistochemistry. Results: Dietary GOS counteracted the DON-induced IL-33 mRNA expression and changed the IL-33 distribution pattern in the mouse small intestine. The IL-33 mRNA expression was positively correlated to the intestinal permeability. A strong positive correlation was also observed between IL-33 mRNA expression in the lung and the number of bronchoalveolar fluid cells. Reduced levels of IL-33 protein, altered IL-33 distribution and reduced ST2 mRNA expression were observed in the lungs of HDM-allergic mice after GOS intervention. Conclusions: Dietary GOS mitigated IL-33 at the mucosal surfaces in a murine model for intestinal barrier dysfunction and HDM-induced asthma. This promising effect may open up new avenues to use GOS not only as a prebiotic in infant nutrition, but also as a functional ingredient that targets inflammatory processes and allergies associated with IL-33 expression.

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Immune Response of Fructo and Galacto‐Oligosaccharides

Vijayasarathy¹,

Prabhu²,

Pavithra³

et al. 2022

Prebiotics and Probiotics in Disease Regulation and Management

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Galacto-oligosaccharides Protect the Intestinal Barrier by Maintaining the Tight Junction Network and Modulating the Inflammatory Responses after a Challenge with the Mycotoxin Deoxynivalenol in Human Caco-2 Cell Monolayers and B6C3F1 Mice

Abstract: The results demonstrate that GOSs stimulate the tight junction assembly and in turn mitigate the deleterious effects of deoxynivalenol on the intestinal barrier of Caco-2 cells and on villus architecture of B6C3F1 mice.

Cited by 115 publications

References 67 publications

Non-digestible carbohydrates in infant formula as substitution for human milk oligosaccharide functions: Effects on microbiota and gut maturation

Non-digestible carbohydrates in infant formula as substitution for human milk oligosaccharide functions: Effects on microbiota and gut maturation

Inflammation-Induced Expression of the Alarmin Interleukin 33 Can Be Suppressed by Galacto-Oligosaccharides

Immune Response of Fructo and Galacto‐Oligosaccharides

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