Gait analysis using accelerometry in dystrophin-deficient dogs

Barthélémy, I.; Barrey, Éric; Thibaud, Jean-Laurent; Uriarte, Ane; Voït, Thomas; Blot, Stéphane; Hogrel, Jean‐Yves

doi:10.1016/j.nmd.2009.07.014

Cited by 52 publications

(77 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The gold standard methods used for clinical assessment of DMD patients, such as the 6-minute walk test, 81 were shown to be difficult to set up in the canine model. 82 Also, the functional quantitative methods using kinematics and accelerometry that were recently published 82,83 enable the comparison of the gait between GRMD dogs and healthy ones. Further investigations will be necessary to determine whether they could represent reliable tools to assess the efficacy of MuStem cell therapy in GRMD dogs.…”

Section: Discussionmentioning

confidence: 99%

Systemic Delivery of Allogenic Muscle Stem Cells Induces Long-Term Muscle Repair and Clinical Efficacy in Duchenne Muscular Dystrophy Dogs

Rouger

Larcher

Dubreil

et al. 2011

The American Journal of Pathology

View full text Add to dashboard Cite

Duchenne muscular dystrophy (DMD) is a genetic progressive muscle disease resulting from the lack of dystrophin and without effective treatment. Adult stem cell populations have given new impetus to cellbased therapy of neuromuscular diseases. One of them, muscle-derived stem cells, isolated based on delayed adhesion properties, contributes to injured muscle repair. However, these data were collected in dystrophic mice that exhibit a relatively mild tissue phenotype and clinical features of DMD patients. Here, we characterized canine delayed adherent stem cells and investigated the efficacy of their systemic delivery in the clinically relevant DMD animal model to assess potential therapeutic application in humans. Delayed adherent stem cells, named MuStem cells (muscle stem cells), were isolated from healthy dog muscle using a preplating technique. In vitro, MuStem cells displayed a large expansion capacity, an ability to proliferate in suspension, and a multilineage differentiation potential. Phenotypically, they corresponded to early myogenic progenitors and uncommitted cells. When injected in immunosuppressed dystrophic dogs, they contributed to myofiber regeneration, satellite cell replenishment, and dystrophin expression. Importantly, their systemic delivery resulted in long-term dystrophin expression, muscle damage course limitation with an increased regeneration activity and an interstitial expansion restriction, and persisting stabilization of the dog's clinical status. These results demonstrate that MuStem cells could provide an attractive therapeutic avenue for DMD patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Systemic Delivery of Allogenic Muscle Stem Cells Induces Long-Term Muscle Repair and Clinical Efficacy in Duchenne Muscular Dystrophy Dogs

Rouger

Larcher

Dubreil

et al. 2011

The American Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…These results support our data about the potential of dogs as an animal model in stem cell researches, and encourage the establishment of the canine model to compare with disease such as those seen in humans. Using this canine x-linked muscular dystrophy model, recent data presented controversies related to the real phenotypes variability presented by these dogs , Barthélémy et al 2009) then pre-clinical results must be interpreted with care and attention. Dog's OE explants maintained in culture in D-MEM/F-12 for 60-day, demonstrated high rate of releasing cells in culture after 24 hours.…”

Section: Culture Of Olfactory Epithelium From 60-day Aged Dogsmentioning

confidence: 99%

Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium

et al. 2010

View full text Add to dashboard Cite

Miglino M.A. 2010. Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium. Pesquisa . Departamento de Ciência Animal, Campus Universitário, Bairro Cibrazen, Bom Jesus, PI 64900-000, Brazil. E-mail: flavioribeiro@ufpi.edu.br A morphological and cell culture study from nasal mucosa of dogs was performed in order to establish a protocol to obtain a cell population committed to neuronal lineage, as a proposal for the treatment of traumatic and degenerative lesions in these animals, so that in the future these results could be applied to the human species. Twelve mongrel dogs of 60-day aged pregnancy were collected from urban pound dogs in São Paulo. Tissue from cribriform ethmoidal lamina of the fetuses was collected at necropsy under sterile conditions around 1h to 2h postmortem by uterine sections and sections from the fetal regions described above. Isolated cells of this tissue were added in DMEM/F-12 medium under standard conditions of incubation (5% CO 2 , >37°C). Cell culture based on isolated cells from biopsies of the olfactory epithelium showed rapid growth when cultured for 24 hours, showing phase-bright sphere cells found floating around the fragments, attached on culture flasks. After 20 days, a specific type of cells, predominantly ellipsoids or fusiform cells was characterized in vitro. The indirect immunofluorescence examination showed cells expressing markers of neuronal precursors (GFAP, neurofilament, oligodendrocyte, and III â-tubulin). The cell proliferation index showed Ki67 immunostaining with a trend to label cell groups throughout the apical region, while PCNA immunostaining label predominantly cell groups lying above the basal lamina. The transmission electron microscopy from the olfactory epithelium of dogs revealed cells with electron-dense cytoplasm and preserving the same distribution as those of positive cell staining for PCNA. Metabolic activity was confirmed by presence of euchromatin in the greatest part of cells. All these aspects give subsidies to support the hypothesis about resident progenitor cells among the basal cells of the olfactory epithelium, committed to renewal of these cell populations, especially neurons. RESUMO.-[Estabelecimento de um protocolo para obtenção de linhagens de células-tronco neuronais a partir do epitélio olfatório de cães.] Foi realizado um estudo morfológico e por cultivo celular a partir de células provenientes da mucosa olfatória de cães, como forma de estabelecer um protocolo de cultivo, como uma proposta para o tratamento de lesões traumáticas e nervosas degenerativas nestes animais e futuramente, para que tais resultados possam ser aplicados a espécie humana. Foram utilizados doze cães sem raça definida, a termo, oriundos de castrações do Centro de Controle de Zoonoses de São Paulo. O tecido da lâmina cribiforme do etmóide dos fetos foi coletado sob necropsia, em condições estéreis, 1 a 2 horas post mortem, por meio de incisão uterina e acesso da região fetal supracitada. Depois as células isol...

show abstract

“…For example, if accelerometer data indicated signs of physical distress such as unusually high or low activity or limping, then electronic alerts could be sent to conservation biologists and wildlife veterinarians. Indeed, it has already been shown for dogs and dairy cattle that accelerometers can distinguish normal from impaired walking (Barthélémy et al 2009, Chapinal et al 2011. Additionally, if behavior patterns associated with human−elephant conflict could be characterized (e.g.…”

Section: Introductionmentioning

confidence: 99%

Accelerometers in collars identify behavioral states in captive African elephants Loxodonta africana

Soltis¹,

Wilson²,

Douglas‐Hamilton³

et al. 2012

Endang. Species. Res.

View full text Add to dashboard Cite

Accelerometers are motion-detection devices that, when attached to animals, are capable of detecting body orientation, overall activity levels, and specific behavior patterns. We deployed accelerometers in order to study the hypothesis that accelerometer output would allow us to distinguish between 4 behavior patterns in 3 adult female African elephants Loxodonta africana at Disney's Animal Kingdom ® , Florida, USA. Tri-axial accelerometer data loggers were attached to the tops of collars worn around the elephants' necks. Behavior was documented on video while the accelerometer output was stored on the data logger. Feeding, bathing, and walking behaviors were recorded in all 3 subjects, while swaying behavior could be recorded in only 1 subject. Data in the 3 physical dimensions (sway, surge, and heave) were analyzed in terms of overall magnitude of movement (dynamic acceleration) and in terms of the periodicity of movement. When classifying accelerometer data of unlabeled origin to the correct behavioral state, overall success ranged from 70 to 91%. Bathing was sometimes confused with feeding and walking, but feeding, walking, and swaying were easily distinguished from each other. These results show that data from accelerometers can distinguish an elephant's behavioral states, and thus may be used to monitor elephant behavior remotely. Such devices could be deployed for a variety of purposes, ranging from monitoring elephant activity in zoos to an early-warning system that could alert the authorities when wild elephants are being illegally hunted. KEY WORDS: Behavior measurement · Accelerometry · Elephant managementResale or republication not permitted without written consent of the publisher OPEN PEN

show abstract

Gait analysis using accelerometry in dystrophin-deficient dogs

Cited by 52 publications

References 45 publications

Systemic Delivery of Allogenic Muscle Stem Cells Induces Long-Term Muscle Repair and Clinical Efficacy in Duchenne Muscular Dystrophy Dogs

Systemic Delivery of Allogenic Muscle Stem Cells Induces Long-Term Muscle Repair and Clinical Efficacy in Duchenne Muscular Dystrophy Dogs

Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium

Accelerometers in collars identify behavioral states in captive African elephants Loxodonta africana

Contact Info

Product

Resources

About