Gaining Insight into Plant Responses to Beneficial and Pathogenic Microorganisms Using Metabolomic and Transcriptomic Approaches

Romero, Fernando Matías; Marina, Marı́a Luisa; Pieckenstain, Fernando Luis; Rossi, Franco Rubén; González, María Elisa Alén; Vignatti, Paulina; Gárriz, Andrés

doi:10.1007/978-981-10-5511-9_6

Cited by 8 publications

(7 citation statements)

References 134 publications

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“…Plant cells are equipped with pattern-recognizing receptors (PRRs), i.e., transmembrane proteins with ectodomains binding ligands of microbial origin-pathogen-/microbe-associated molecular patterns (P/MAMPs) [70]. The recognition of a ligand leads to the activation of plant pattern-triggered immunity (PTI), which limits the development of microorganisms in plant tissues, e.g., through an increased synthesis of antimicrobial compounds [71]. To date, a number of MAMP molecules produced by the fungi of the Trichoderma genus have been identified [72,73], including hydrolytic enzymes that allow for the penetration of the outermost layers of root tissues, such as cellulases [74], xylanases [75], and polygalacturonases [76].…”

Section: Discussionmentioning

confidence: 99%

Trichoderma viride Colonizes the Roots of Brassica napus L., Alters the Expression of Stress-Responsive Genes, and Increases the Yield of Canola under Field Conditions during Drought

Garstecka,

Antoszewski,

Mierek-Adamska

et al. 2023

IJMS

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In this work, we present the results of the inoculation of canola seeds (Brassica napus L.) with Trichoderma viride strains that promote the growth of plants. Seven morphologically different strains of T. viride (TvI-VII) were shown to be capable of synthesizing auxins and exhibited cellulolytic and pectinolytic activities. To gain a deeper insight into the molecular mechanisms underlying canola–T. viride interactions, we analyzed the canola stress genes metallothioneins (BnMT1-3) and stringent response genes (BnRSH1-3 and BnCRSH). We demonstrated the presence of cis-regulatory elements responsive to fungal elicitors in the promoter regions of B. napus MT and RSH genes and observed changes in the levels of the transcripts of the above-mentioned genes in response to root colonization by the tested fungal strains. Of the seven tested strains, under laboratory conditions, T. viride VII stimulated the formation of roots and the growth of canola seedlings to the greatest extent. An experiment conducted under field conditions during drought showed that the inoculation of canola seeds with a suspension of T. viride VII spores increased yield by 16.7%. There was also a positive effect of the fungus on the height and branching of the plants, the number of siliques, and the mass of a thousand seeds. We suggest that the T. viride strain TvVII can be used in modern sustainable agriculture as a bioinoculant and seed coating to protect B. napus from drought.

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Section: Discussionmentioning

confidence: 99%

Trichoderma viride Colonizes the Roots of Brassica napus L., Alters the Expression of Stress-Responsive Genes, and Increases the Yield of Canola under Field Conditions during Drought

Garstecka,

Antoszewski,

Mierek-Adamska

et al. 2023

IJMS

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“…Xu et al [ 107 ] performed a study using RNA-sequencing to observe plants' ability to produce genes mediating defense reactions against Verticillium dahlia, a root blighting pathogen. They uncovered a network of signal transduction pathways using microarray-based plant transcriptomics, which gets triggered due to elicitors constituting pathogen-associated molecular patterns (PAMPs) and signaling compounds such as SA, ABA, JA, and ET [ 108 , 109 ]. These studies have resulted in the discovery of key regulatory and resistance genes [ 110 ].…”

Section: Molecular Techniques For Decoding Plant-microbiome Interactimentioning

confidence: 99%

Deciphering the Omics of Plant-Microbe Interaction: Perspectives and New Insights

Sharma

Sudheer

Usmani

et al. 2020

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: Plants do not grow in isolation, rather they are also hosts to a variety of microbes in their natural environments. While, few thrive in the plants for their sole benefit, others may have a direct bearing on plants in a symbiotic manner. Unravelling plant-microbe interactions is a crucial component to recognize the beneficial and detrimental impact of microbes on plants. Also, by affecting the environment around plants, microbes may indirectly influence the plants. The progress in sequencing technologies in the genomics era and several omics tool has grown in biological science. Studying the complex nature of plant-microbe interactions can offer several strategies to increase productivity of plants in an environmentally friendly manner by providing better insights. This review brings forward the recent works performed in building omics strategies that decipher the interactions between plantmicrobiome. At the same time, this study further explores other associated mutually beneficial aspects of plant-microbe interactions such as plant growth promotion, nitrogen fixation, stress suppressions in crops and bioremediation. This work also provides a better intelligence about metabolic interactions between the microbe and the plant through omics approaches. The review explores advances in the study of Arabidopsis as an important avenue to serve as a baseline tool to create models that help in scrutinizing various factors that contribute to the elaborate relationship between the plant and microbes. Causal relationships between plants and microbes can be established through systematic gnotobiotic experimental studies to test hypotheses on biologically derived interactions. This review will bring forth recent advances in the study of plant-microbe interactions keeping in view the advantages of these interactions in improving nutrient uptake and plant health.

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“…Xu et al [107] performed a study using RNAsequencing to observe plants' ability to produce genes mediating defense reactions against Verticillium dahlia, a root blighting pathogen. They uncovered a network of signal transduction pathways using microarray-based plant transcriptomics, which gets triggered due to elicitors constituting pathogen-associated molecular patterns (PAMPs) and signaling compounds such as SA, ABA, JA, and ET [108,109]. These studies have resulted in the discovery of key regulatory and resistance genes [110].…”

Section: Transcriptome Analysis Of Plant-pathogen Interactionsmentioning

confidence: 99%

Deciphering the Omics of Plant-Microbe Interaction: Perspectives and New Insights

Sharma¹

2020

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Introduction: Plants do not grow in isolation, rather they are hosts to a variety of microbes in their natural environments. While, few thrive in the plants for their own benefit, others may have a direct impact on plants in a symbiotic manner. Unraveling plant-microbe interactions is a critical component in recognizing the positive and negative impacts of microbes on plants. Also, by affecting the environment around plants, microbes may indirectly influence plants. The progress in sequencing technologies in the genomics era and several omics tools has accelerated in biological science. Studying the complex nature of plant-microbe interactions can offer several strategies to increase the productivity of plants in an environmentally friendly manner by providing better insights. This review brings forward the recent works performed in building omics strategies that decipher the interactions between plant-microbiome. At the same time, it further explores other associated mutually beneficial aspects of plant-microbe interactions such as plant growth promotion, nitrogen fixation, stress suppressions in crops and bioremediation; as well as provides better insights on metabolic interactions between microbes and plants through omics approaches. It also aims to explore advances in the study of Arabidopsis as an important avenue to serve as a baseline tool to create models that help in scrutinizing various factors that contribute to the elaborate relationship between plants and microbes. Causal relationships between plants and microbes can be established through systematic gnotobiotic experimental studies to test hypotheses on biologically derived interactions. Conclusion:This review will cover recent advances in the study of plant-microbe interactions keeping in view the advantages of these interactions in improving nutrient uptake and plant health.

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Gaining Insight into Plant Responses to Beneficial and Pathogenic Microorganisms Using Metabolomic and Transcriptomic Approaches

Cited by 8 publications

References 134 publications

Trichoderma viride Colonizes the Roots of Brassica napus L., Alters the Expression of Stress-Responsive Genes, and Increases the Yield of Canola under Field Conditions during Drought

Trichoderma viride Colonizes the Roots of Brassica napus L., Alters the Expression of Stress-Responsive Genes, and Increases the Yield of Canola under Field Conditions during Drought

Deciphering the Omics of Plant-Microbe Interaction: Perspectives and New Insights

Deciphering the Omics of Plant-Microbe Interaction: Perspectives and New Insights

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