Gain of chromosome arm 9p is characteristic of primary mediastinal b-cell lymphoma (MBL): Comprehensive molecular cytogenetic analysis and presentation of a novel MBL cell line

MedB-1, a human tumor cell line derived from a primary mediastinal large B-cell lymphoma

“…24 Probing mediastinal B-cell lymphomas by molecular cytogenetics we and others reported frequent overrepresentation of genomic material on chromosomes X and 9, 34,35 the consensus region on chromosome 9 being 9p23-9p24. 36 The Janus kinase 2 gene, Jak2, was recently identified as one of the candidate genes in this genomic region.…”

“…Karyotyping using classical Giemsa-band-ing technique was carried out and revealed 47, XY, inv(X) (p22; q13), ϩder(1)t(1;14) (q10,q10), ϩ9, Ϫ14, Ϫ21, i(21q). 24 …”

Primary mediastinal B‐cell lymphoma

“…The most commonly observed gains are at +9p, +12q and +Xq. [15][16][17] Using array based CGH, where the resolution is greater, more regions of loss have been demonstrated, but these are generally small in size. The median size of regions of loss is 6 Mb, compared to genomic gains which are a median size of 29.5 Mb.…”

“…[16][17][18][19][20] By florescence in situ hybridization (FISH) analysis, genomic gains have been identified in 75% of cases at this loci. 19,29 There is good evidence for constitutive activation of NF-κB pathway in PMBL.…”

“…17 By using FISH gains at this location are observed in 75% of PMBL. 16 In classical HL, 9p gains at an identical consensus region are also recognized. This common region spans the JAK2 locus.…”