Gain of 3q and deletion of 11q22 are frequent aberrations in mantle cell lymphoma

Abstract

We used comparative genomic hybridization (CGH) to screen for DNA copy number changes in 34 specimens from 27 cases of mantle cell lymphoma (MCL). The most common gains were detected at 3q (52%), 8q (30%), and 15q (26%), whereas the most frequent losses involved 13q (41%), 1p (33%), 6q (30%), 9p (30%), and 11q (30%). The gain of 3q, with a minimal common region at 3q26.1‐27, appeared in more than half of the lymphomas, suggesting the location of an important oncogene here. A common deleted region at 11q22 was found in one‐third of the patients, which suggests that this region may harbor a tumor suppressor gene important in the tumorigenesis of MCL. The mean number of changes was higher in more aggressive blastoid variants of MCL than in lymphomas with typical morphology. Our results show that the chromosomal regions affected in MCL are highly consistent and are different from those seen in other types of non‐Hodgkin's lymphoma. Genes Chromosomes Cancer 21:298–307, 1998. © 1998 Wiley‐Liss, Inc.

DNA copy number amplifications in sarcomas with homogeneously staining regions and double minutes

“…Imbalanced chromosomal translocations are associated with DNA copy number amplifications (10,12). Mapping of the breakpoints (data not shown here) involved in the cytogenetic aberrations did not, however, indicate any such relationship, with the possible exception of bands 1q21-q23, 11q14, and 12q13, all of which were frequently involved in rearrangements as well as amplifications.…”

Mantle cell lymphomas with clonal immunoglobulin V3–21 gene rearrangements exhibit fewer genomic imbalances than mantle cell lymphomas utilizing other immunoglobulin V genes

“…Chromosome 9 deletions appeared to be a common finding, which is in agreement with other studies. [9][10][11][12] However, in our material, a dual pattern of losses was found in which the short arm was deleted only in the non-V H 3-21 mantle cell lymphoma, while losses of 9q were found in both V H 3-21 þ and non-V H 3-21 cases. Of note, the deletions on 9p encompass the INK4a/ARF locus at 9p21 that encodes two tumor suppressors, p16 INK4a and p14 ARF .…”

“…This is the most common finding in mantle cell lymphoma in general and is suggested to be an early event in mantle cell lymphomagenesis. [9][10][11][12] Overexpression of BCL6, located at 3q27, is usually not found in spite of a 3q gain. 10 However, our data suggest a minimal gained region of 3q25, which supports the involvement of another oncogene.…”

“…3 Characterization of variable heavy chain (V H ) gene usage has recently been performed in mantle cell lymphoma, revealing that two particular V H gene segments of the immunoglobulin (Ig) locus were preferentially used, namely V H 3-21 (18%) and V H 4-34 (16%). 2,6 Interestingly, the tumor samples with V H 3-21 gene usage almost exclusively expressed lambda light chains, and also in most cases utilized the same V l light chain gene (V l [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. 6 In addition, this group has been shown to have a significantly better prognosis in one report and in other studies displayed a clear tendency towards improved survival compared to mantle cell lymphomas utilizing other V H genes.…”

“…Earlier CGH studies indicate that mantle cell lymphoma constitutes a genetically homogeneous subgroup of lymphoma characterized by recurrent aberrations, in particular gains within 3q and 8q and losses in 6q, 9p, 11q and 13q. [9][10][11][12] An increased number of aberrations in addition to complex karyotypes have also been reported to be associated with poor prognosis in mantle cell lymphoma. 10 In the current series, we have applied the CGH technique to investigate whether the genomic architecture differs between the V H 3-21 þ group as compared to mantle cell lymphoma in general.…”