Gagamyeonryunggobon-dan(Jiājiǎnyánlínggùběn-dān) induces hair regrowth effect from activating hair follicle

Abstract

Objective : The purpose of this study is to report the hair regrowth effect of Gagamyeonryunggobon-dan on ICR mice from measuring the change of diverse factors.Methods : Gagamyeonryunggobon-dan was treated by oral administration with 2.5㎎/㎏/day amount for 3 weeks per mouse everyday. Hair regrowth was estimated by change of morphology, angiogenesis, hair follicle activation. The change of morphology was observed with external, internal change and sebaceous gland. Angiogenesis was estimated by image analysis, capillary distribution and angiogenic chemokine(MIP-2). Hair follicle activation was estimated by PCNA, IGF-2 and serotonin.Results : 1. Gagamyeonryunggobon-dan treated group had more and thicker hairs than the group not treated. Especially well developed sebaceous glands were seen in dermis of treated group. 2. Gagamyeonryunggobon-dan treated group had more capillaries near hair follicles of subcutaneous layer and more 2019% MIP-2 positive activity than the group not treated. 3. Gagamyeonryunggobon-dan treated group increased positive activity up to 596% in PCNA, 187% in IGF-2 and 547% in serotonin more than the group not treated.

Puerarin Improves Dexamethasone-Impaired Wound Healing In Vitro and In Vivo by Enhancing Keratinocyte Proliferation and Migration

“…The skin sections were stained with hematoxylin and eosin (H&E) for evaluation of wound morphology. Masson trichrome (M/T) and phloxine-tartrazine (P/T) staining were performed to examine the fibrotic changes to the dermis and the angiogenesis changes to the epidermis, respectively, according to previous studies [22,23]. Immunohistochemical staining was conducted to evaluate the changes in the fibrotic scarring response related to TGF-β.…”

Phamacopuncture and Dermal Application of Sebalgukhwa-san: Effects on Hair Growth in a Mouse Model of Alopecia

“…In recent years, a mismatch of physical conditions causing abnormalities of the hormone and immune system, have been observed to result in the malnutrition of hair, and circulatory disorders [5]. Traditional medicine treatments for alopecia include herbal medicine [6][7][8], bee venom pharmacopuncture [9], pharmacopuncture [10,11] and the application of Saengbal-eum [12]. In recent years, plum-blossom needling [13], microneedle therapy system [14,15], and threadembedding therapy [16] have also been used.…”