GABAergic terminals are presynaptic to primary afferent terminals in the substantia gelatinosa of the rat spinal cord

Barber, Robert P.; Vaughn, James E.; Saito, Kihachi; McLaughlin, Barbara J.; Roberts, Eugene

doi:10.1016/0006-8993(78)90615-7

Cited by 335 publications

(110 citation statements)

References 49 publications

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“…The GABA immunoreactive subcellular organellae were round synaptic vesicles, and mitochondrial membranes. These GABA-positive sites coincide with those reported by others (MC-LAUGHLIN et al, 1975;BARBER et al, 1978;MAGOUL et al, 1987;KADURI et al, 1987;MERIGHI et al, 1989;CARLTON and HAYES, 1990). In particular, MERIGHI et al clearly demonstrated GABA immunogold colloidal particles over small clear vesicles.…”

Section: Discussionsupporting

confidence: 89%

“…Furthermore, not the all CH-terminals RIBEIRO-DA-SILVA and COIMBRA (1982). GABA immunoreactivity was marked in the superficial laminae of the dorsal horn, as has been reported by many others (MCLAUGHLIN et al, 1975;BARBER et al, 1978BARBER et al, , 1982MAGOUL et al, 1987;KADURI et al, 1987;MERIGHI et al, 1989;TODD and MCKENZIE, 1989;CARLTON and HAYES, 1990; TODD and LOCHIIEAD, 1990). Since no non-specific GABA immunoreactivity was detected in control specimens, this GABA immunoreaction is believed to be specific for neurons containing GABA.…”

Section: Discussionsupporting

confidence: 77%

See 1 more Smart Citation

GABAergic neurons in the mouse superficial dorsal horn with special emphasis on their relation to primary afferent central terminals.

Hiura

Ishizuka

Villalobos

1991

Archives of Histology and Cytology

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 77%

GABAergic neurons in the mouse superficial dorsal horn with special emphasis on their relation to primary afferent central terminals.

Hiura

Ishizuka

Villalobos

1991

Archives of Histology and Cytology

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“…Voltage pulses are applied through a suction electrode (4). The recording electrode is connected to a preamplifier (6). A chlorided silver wire is used for grounding (5).…”

Section: Representative Resultsmentioning

confidence: 99%

“…It inhibits excitatory postsynaptic potentials (EPSPs) in monosynaptically excited motoneurons without changing the postsynaptic membrane potential and the excitability of the motoneurons [1][2][3] . Primary afferent depolarization (PAD) induced by GABAergic axo-axonal synapses onto sensory presynaptic fibers is the underlying mechanism [4][5][6][7] (see also Figure1a). These synapses contain GABA A-and GABA B -receptors (GABA A R and GABA B R).…”

Section: Introductionmentioning

confidence: 99%

Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording <em>In Vivo</em>

Grünewald

Geis

2014

JoVE

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Presynaptic inhibition is one of the most powerful inhibitory mechanisms in the spinal cord. The underlying physiological mechanism is a depolarization of primary afferent fibers mediated by GABAergic axo-axonal synapses (primary afferent depolarization). The strength of primary afferent depolarization can be measured by recording of volume-conducted potentials at the dorsal root (dorsal root potentials, DRP). Pathological changes of presynaptic inhibition are crucial in the abnormal central processing of certain pain conditions and in some disorders of motor hyperexcitability. Here, we describe a method of recording DRP in vivo in mice. The preparation of spinal cord dorsal roots in the anesthetized animal and the recording procedure using suction electrodes are explained. This method allows measuring GABAergic DRP and thereby estimating spinal presynaptic inhibition in the living mouse. In combination with transgenic mouse models, DRP recording may serve as a powerful tool to investigate disease-associated spinal pathophysiology. In vivo recording has several advantages compared to ex vivo isolated spinal cord preparations, e.g. the possibility of simultaneous recording or manipulation of supraspinal networks and induction of DRP by stimulation of peripheral nerves. Video LinkThe video component of this article can be found at

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“…It is thus possible the dorsal horn intemeurons may upregulate two coexisting messenger molecules, GABA and NPY, after peripheral inflammation. GABA nerve endings form axoaxonic contacts with primary afferent boutons (Barber et al, 1978;Todd and Lockhead, 1990), and GABA, and GABA, receptors are present on the central nerve endings of primary afferents (Singer and Placheta, 1980;Price et al, 1984). It may therefore be that NPY and GABA, in a concerted action via presynaptic effects, inhibit transmission in the dorsal horn.…”

Section: Comparison With Axotomymentioning

confidence: 99%

Expression of neuropeptide Y and neuropeptide Y (Y1) receptor mRNA in rat spinal cord and dorsal root ganglia following peripheral tissue inflammation

et al. 1994

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By using in situ hybridization histochemistry and immunohistochemistry, neuropeptide Y (NPY) and NPY (Y1) receptor mRNA as well as NPY-like immunoreactivity were examined in the lumbar spinal cord (L4-L5) and in dorsal root ganglia (DRG, L5) in rats injected with complete Freund's adjuvant (CFA) into the hindpaw. A rapid and marked increase in NPY mRNA expression was observed in ipsilateral dorsal horn neurons 6 hr after inoculation as compared to the contralateral side. This was mainly found in the medial part of spinal lamina II. The peak level (88% increase) was reached at 3 d. In adjacent sections of the spinal cord, 96% and 33% increases were found in the number of dynorphin and enkephalin mRNA-positive neurons, respectively. Unilateral inflammation also induced a moderate increase in NPY-like immunoreactivity and the number of NPY-immunoreactive neurons in the medial part of the ipsilateral spinal dorsal horn. In addition, a marked elevation in the expression of c-Fos-like protein was observed in ipsilateral spinal neurons in laminae I, II, and V. However, no NPY mRNA-positive or NPY- immunoreactive neurons were found in the ipsilateral and contralateral DRGs in rats receiving CFA injection. Furthermore, a marked upregulation of NPY (Y1) receptor mRNA expression was detected in the ipsilateral spinal dorsal horn 1 d and 3 d after inoculation. These Y1 receptor mRNA-positive cells were mainly distributed in the medial laminae II and III. Numerous Y1 mRNA-positive, small neuron profiles were found bilaterally in the DRGs in CFA-treated rats. CFA evoked a 34% increase in the number of Y1 mRNA-positive neurons in ipsilateral DRGs as compared to contralateral DRGs. The distinct upregulation of NPY and NPY (Y1) receptor in response to peripheral inflammation suggests an involvement of NPY in the response to inflammation and in nociception.

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GABAergic terminals are presynaptic to primary afferent terminals in the substantia gelatinosa of the rat spinal cord

Cited by 335 publications

References 49 publications

GABAergic neurons in the mouse superficial dorsal horn with special emphasis on their relation to primary afferent central terminals.

GABAergic neurons in the mouse superficial dorsal horn with special emphasis on their relation to primary afferent central terminals.

Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording <em>In Vivo</em>

Expression of neuropeptide Y and neuropeptide Y (Y1) receptor mRNA in rat spinal cord and dorsal root ganglia following peripheral tissue inflammation

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