GABAB receptors at glutamatergic synapses in the rat striatum

Lacey, Carolyn J.; Boyes, Justin; Gerlach, Oliver; Chen, L.; Magill, Peter J.; Bolam, J. Paul

doi:10.1016/j.neuroscience.2005.07.013

Cited by 104 publications

(101 citation statements)

References 86 publications

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“…Electron microscopic analysis revealed that VGluT1 and VGluT2 immunogold labeling was concentrated within axons and axon terminals forming asymmetric synapses. Consistent with previous studies in rat (Lacey et al, 2005;Fujiyama et al, 2006;Raju et al, 2006;Moss and Bolam, 2008) and rhesus monkey (Raju et al, 2008) (Table 1), VGluT1-positive terminals in striatal tissue from both D 1 and D 2 transgenic mice formed asymmetric synapses almost exclusively with spines (98.8 and 98.2%, respectively), with only a small proportion in synaptic contact with dendritic shafts (Table 1). Likewise, VGluT2-positive terminals in the D 1 and D 2 tissue had a similar distribution to other species, with ϳ77-80% in contact with spines and the remainder (ϳ21%) in contact with dendritic shafts (Table 1).…”

Section: Postsynaptic Targets Of Corticostriatal and Thalamostriatal supporting

confidence: 90%

“…The VGluT primary antibodies were raised against rat VGluT1 and VGluT2 (amino acids 543-560 and 510-582, respectively). The distribution of immunolabeling at the light and electron microscopic levels was distinct for each primary antibody and consistent with previous observations by ourselves (Lacey et al, 2005;Moss and Bolam, 2008) and others, using the same or different antibodies (Fremeau et al, 2001;Herzog et al, 2001;Kaneko and Fujiyama, 2002;Fujiyama et al, 2004Fujiyama et al, , 2006Raju et al, 2006Raju et al, , 2008. No immunolabeling was observed after omission of the primary antibodies or when tissue from wild-type littermates (D 1 BAC-EGFP negative) was processed using the anti-GFP antibody, followed by a tungstate-stabilized TMB reaction.…”

Section: Immunohistochemistrysupporting

confidence: 90%

“…The targets of cortical and thalamic innervation of the striatum, as indicated by labeling for VGluT1 or VGluT2, respectively, have been studied extensively in rat and nonhuman primate (Lacey et al, 2005;Fujiyama et al, 2006;Raju et al, 2006Raju et al, , 2008Moss and Bolam, 2008). To determine whether the synaptic architecture in the D 1 and D 2 transgenic mice is comparable with that in other species, we analyzed tissue from the D 1 and D 2 BAC-EGFP mice single immunolabeled for VGluT1 or VGluT2.…”

Section: Postsynaptic Targets Of Corticostriatal and Thalamostriatal mentioning

confidence: 99%

“…The predominant targets of corticostriatal and thalamostriatal projections are the dendritic spines of the medium-sized spiny neurons (MSNs), the main neuronal cell-type and principal projection neuron of the striatum (Kemp and Powell, 1971a,b;Somogyi et al, 1981;Dubé et al, 1988;Xu et al, 1989;Smith and Bolam, 1990;Xu et al, 1991;Sadikot et al, 1992;Smith et al, 1994Smith et al, , 2004Bolam et al, 2000;Lacey et al, 2005;Raju et al, 2006Raju et al, , 2008Moss and Bolam, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Cortical and Thalamic Innervation of Direct and Indirect Pathway Medium-Sized Spiny Neurons in Mouse Striatum

Doig¹,

Moss²,

Bolam³

2010

J. Neurosci.

203

173

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The striatum receives major excitatory inputs from the cortex and thalamus that predominantly target the spines of medium-sized spiny neurons (MSNs). We aimed to determine whether there is any selectivity of these two excitatory afferents in their innervation of direct and indirect pathway MSNs. To address this, we used bacterial artificial chromosome transgenic mice, in which enhanced green fluorescent protein (EGFP) reports the presence of D 1 or D 2 dopamine receptor subtypes, markers of direct and indirect pathway MSNs, respectively. Excitatory afferents were identified by the selective expression of vesicular glutamate transporter type 1 (VGluT1) by corticostriatal afferents and vesicular glutamate transporter type 2 (VGluT2) by thalamostriatal afferents. A quantitative electron microscopic analysis was performed on striatal tissue from D 1 and D 2 mice that was double immunolabeled to reveal the EGFP and VGluT1 or VGluT2. We found that the proportion of synapses formed by terminals derived from the cortex and thalamus was similar for both direct and indirect pathway MSNs. Furthermore, qualitative analysis revealed that individual cortical or thalamic terminals form synapses with both direct and indirect pathway MSNs. Similarly, we observed a convergence of cortical and thalamic inputs onto individual MSNs of both direct and indirect pathway: individual EGFP-positive structures received input from both VGluT2-positive and VGluT2-negative terminals. These findings demonstrate that direct and indirect pathway MSNs are similarly innervated by cortical and thalamic afferents; both projections are thus likely to be critical in the control of MSNs and hence play fundamental roles in the expression of basal ganglia function.

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Section: Postsynaptic Targets Of Corticostriatal and Thalamostriatal supporting

confidence: 90%

Section: Immunohistochemistrysupporting

confidence: 90%

Section: Postsynaptic Targets Of Corticostriatal and Thalamostriatal mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cortical and Thalamic Innervation of Direct and Indirect Pathway Medium-Sized Spiny Neurons in Mouse Striatum

Doig¹,

Moss²,

Bolam³

2010

J. Neurosci.

203

173

View full text Add to dashboard Cite

show abstract

“…Glutamate thus possesses a dual role in modulating dopaminergic and GABAergic activity. DA and GABA subsequently regulate glutamatergic activity via activation of their receptors on the glutamatergic terminals [8,17]. This forms a self-contained regulation mechanism which efficiently checks the activity level among the Glu/DA/GABA neurons in the striatum.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of stimulated dopamine release and hemodynamic response in the brain through electrical stimulation of rat forepaw

Chen

Ren

Wang

et al. 2008

Neuroscience Letters

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The subcortical response to peripheral somatosensory stimulation is not well studied. Prior literature suggests that somatosensory stimulation can affect dopaminergic tone. We studied the effects of electrical stimulation near the median nerve on the response to an amphetamine induced increase in synaptic dopamine. We applied the electrical stimulation close to the median nerve 20 minutes after administration of 3mg/kg amphetamine. We used fMRI and microdialysis to measure markers of DA release, together with the release of associated neurotransmitters of striatal Glutamate (Glu) and GABA.Result-1) Changes in cerebral blood volume (CBV), a marker used in fMRI, indicate that electrical stimulation significantly attenuated increased DA release (due to AMPH) in the striatum, thalamus, medial prefrontal and cingulate cortices. 2) Microdialysis showed that electrical stimulation increased Glu and GABA release and attenuated the AMPH-enhanced DA release. The striatal DA dynamics correlated with the CBV response.Conclusion-These results demonstrate that electrical stimulation near the median nerve activates Glu/GABA release which subsequently attenuate excess striatal DA release. These data provide evidence for physiologic modulation caused by electroacupuncture at points near the median nerve.

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Effects of Exercise on Basal Ganglia Function in Parkinson's Disease and Its Animal Models

Petzinger

Fisher

Meshul

et al. 2011

Parkinson's Disease

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GABAB receptors at glutamatergic synapses in the rat striatum

Cited by 104 publications

References 86 publications

Cortical and Thalamic Innervation of Direct and Indirect Pathway Medium-Sized Spiny Neurons in Mouse Striatum

Cortical and Thalamic Innervation of Direct and Indirect Pathway Medium-Sized Spiny Neurons in Mouse Striatum

Inhibition of stimulated dopamine release and hemodynamic response in the brain through electrical stimulation of rat forepaw

Effects of Exercise on Basal Ganglia Function in Parkinson's Disease and Its Animal Models

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