GABA transport in the rat thyroid

Gebauer, H.

doi:10.1007/bf00506258

Cited by 16 publications

(8 citation statements)

References 34 publications

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“…Kinetic analysis indicates that GABA is transported by a saturable uptake process. The mechanism of 3H-GABA uptake in the ante rior pituitary was of lower efficiency than in cerebral cor tex [14], However, kinetic parameters of 3H-GABA up take in the anterior pituitary are in agreement with data reported for nonneuronal tissues [12]. Anterior pituitary 3H-GABA uptake was sensitive to the inhibition pro duced by the GABA-related compounds that are known to inhibit neuronal and/or glial GABA uptake [19][20][21].…”

Section: Discussionsupporting

confidence: 78%

“…These results show that the anterior pituitary gland can accumulate 3H-GABA in a temperature-and sodiumdependent manner, and this was similar to the GABA uptake by other tissues [12,14,17,18]. Kinetic analysis indicates that GABA is transported by a saturable uptake process.…”

Section: Discussionsupporting

confidence: 51%

“…The block ade of GABA-T activity did not modify 'H-GABA up take. In addition a very low degradation of GABA was detected at short-time 'H-GABA uptake studies in brain slices [14] and thyroid [12], therefore radioactivity in the tissue was considered as 'H-GABA.…”

Section: Time Course O F3h-gaba Uptakementioning

confidence: 99%

“…More over, GABA has also been found in several nonneuronal tissues, although at much lower concentrations than in nervous tissues [see 11]. In addition, some nonneuronal tissues such as thyroid [12] and pancreatic islets [1 1 ] dis play the ability to take up GABA by a transport mecha nism similar to that of neural GABA uptake. A prelimina ry report showed the presence of a sodium-dependent pro cess capable of accumulating 3H-GABA in rat anterior pituitary homogenates [13],…”

Section: Introductionmentioning

confidence: 99%

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GABA Transport and Subcellular Distribution in the Rat Anterior Pituitary Gland

Duvilanski

Seilicovich²,

Debéljuk³

et al. 1994

Neuroendocrinology

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In this investigation we have studied the uptake of gamma aminobutyric acid (GABA) into anterior pituitary slices. Tissue:medium ratios of about 45:1 were obtained after a 30-min incubation. The process responsible for ³H-GABA uptake was temperature-sensitive and sodium-dependent. The kinetic constants of saturable GABA transport were: K_m 4.141 µM and V_max 0.973 pmol/min/mg protein, at 25 °C. The incorporation of GABA into anterior pituitary was inhibited by specific inhibitors of neuronal and/or glial uptake. The subcellular distribution of GABA was investigated by continuous sucrose density gradients and differential centrifugation. Most of the endogenous and labelled GABA was present in the soluble fraction. However, a small part of GABA was found in the particulate fraction. These observations indicate that the anterior pituitary gland is able to concentrate GABA which interacts with intracellular particles.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 51%

Section: Time Course O F3h-gaba Uptakementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

GABA Transport and Subcellular Distribution in the Rat Anterior Pituitary Gland

Duvilanski

Seilicovich²,

Debéljuk³

et al. 1994

Neuroendocrinology

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“…GABA appears to play a role in the homeostasis of the normal thyroid [13]. The thyroid gland has been shown to both actively take up and synthesize GABA [14,15]. Research in rodent models suggests a complex interaction between thyroid hormone and GABA, with each regulating secretion or uptake of the other.…”

Section: Introductionmentioning

confidence: 97%

GABA Receptor Expression in Benign and Malignant Thyroid Tumors

Roberts

Mendonca-Torres

Jensen

et al. 2009

Pathol. Oncol. Res.

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Neurotransmitter systems have recently been shown to be involved in multiple malignancies including breast, colon and prostate cancers. The role of neurotransmitters and neurotrophic factors has not yet been examined in thyroid cancer. To determine the possible involvement of neurotransmitter systems in thyroid carcinogenesis we characterized the patterns of gamma-aminobutyric acid (GABA) receptor expression in normal thyroid and thyroid tumors. We examined the expression patterns of the GABAergic system in 70 human thyroid tumor samples (13 follicular adenomas, 14 follicular carcinomas, 43 papillary carcinomas) and adjacent normal thyroid by immunohistochemistry. GABAergic system mRNA expression in thyroid cancer cell lines derived from primary (FTC133) and metastatic tumors (FTC236 and FTC238) was examined by real time PCR. Overall, GABA receptor expression is increased in tumors compared to normal thyroid tissue. Expression of GABAA receptor beta2 was detected in the vasculature of normal thyroid and thyroid tumors but not in thyroid cancer cells. GABAA alpha2 was detected in metastatic-derived but not in primary-tumor derived cell lines. Expression levels of GABAB R2 and GABA receptor associated protein (GABARAP) are increased in adenomas and thyroid cancer suggesting their role in early stages of thyroid tumorigenesis. This study represents the first demonstration of GABA receptor expression in human thyroid tissue and suggests that the GABAergic system is involved in thyroid carcinogenesis.

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Autoradiographic distribution of radioactivity from14C-GABA in the mouse

Kuroda

Watanabe

Tamayama

et al. 2000

Microsc. Res. Tech.

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We investigated the distribution of radioactivity from 14C‐labeled γ‐aminobutyric acid (GABA) in the mouse by in vivo autoradiography to clarify the tissues that show GABA uptake and/or GABA binding. Male mice were injected intravenously with 14C‐GABA in both the absence and presence of an excess of unlabeled GABA, baclofen and isoguvacine. Whole‐body autoradiography of 3H‐baclofen, a GABAB receptor agonist was also performed. At short intervals after 14C‐GABA injection ( 3 and 6 minutes), very high radioactivity was detected in the kidney cortex, liver, pineal gland, hypophysis, median eminence of the hypothalamus, and cervical ganglion. The hyaline cartilage and glandular part of the stomach showed moderate radioactivity. In the presence of an excess amount of unlabeled GABA, radioactivity in most of tissues decreased significantly, but no significant difference in radioactivity was observed in the presence of baclofen and isoguvacine, agonists of GABAA and GABAB receptors, respectively. Autoradiography of 3H‐baclofen showed that the kidney had high level of radioactivity, whereas the activity in other tissues and organs was similar or lower than in the blood except for the content of the urinary bladder and the pancreas at 15 minutes after injection. These data indicate that radioactivity from incorporated 14C‐GABA into a variety of cells is much higher than that from bound 14C‐GABA to the receptor sites. Our results suggest that GABA can be quickly localized in many organs of the mouse body after 3 minutes following injection, and GABA may serve multiple functions in those organs. Microsc. Res. Tech. 41:116–126, 2000. © 2000 Wiley‐Liss, Inc.

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GABA transport in the rat thyroid

Cited by 16 publications

References 34 publications

GABA Transport and Subcellular Distribution in the Rat Anterior Pituitary Gland

GABA Transport and Subcellular Distribution in the Rat Anterior Pituitary Gland

GABA Receptor Expression in Benign and Malignant Thyroid Tumors

Autoradiographic distribution of radioactivity from14C-GABA in the mouse

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