GABA enzymatic assay kit

Nishiyama, Tatsuya; Sulistyaningdyah, Woro Triarsi; Uéda, Kenji; Kusakabe, Hitoshi

doi:10.1080/09168451.2019.1661768

Cited by 11 publications

(3 citation statements)

References 18 publications

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“…After additional incubation, the absorbance was measured at 555 nm. The contents of gamma-aminobutyric acid were determined with reference to the standard curve [ 67 ].…”

Section: Methodsmentioning

confidence: 99%

“…For the reaction, 0.5 mL of solution I was added to 50 µL of sample and incubated for 10 min at 30 • C. After incubation, 0.5 mL of solution II was added and incubated for 10 min at 30 • C. After additional incubation, the absorbance was measured at 555 nm. The contents of gamma-aminobutyric acid were determined with reference to the standard curve [67].…”

Section: Measurement Of Gamma-aminobutyric Acid Contentsmentioning

confidence: 99%

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Proteomic Analysis Reveals Salt-Tolerant Mechanism in Soybean Applied with Plant-Derived Smoke Solution

Komatsu,

Kimura,

Rehman

et al. 2023

IJMS

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Salt stress of soybean is a serious problem because it reduces plant growth and seed yield. To investigate the salt-tolerant mechanism of soybean, a plant-derived smoke (PDS) solution was used. Three-day-old soybeans were subjected to PDS solution under 100 mM NaCl for 2 days, resulting in PDS solution improving soybean root growth, even under salt stress. Under the same condition, proteins were analyzed using the proteomic technique. Differential abundance proteins were associated with transport/formaldehyde catabolic process/sucrose metabolism/glutathione metabolism/cell wall organization in the biological process and membrane/Golgi in the cellular component with or without PDS solution under salt stress. Immuno-blot analysis confirmed that osmotin, alcohol dehydrogenase, and sucrose synthase increased with salt stress and decreased with additional PDS solution; however, H+ATPase showed opposite effects. Cellulose synthase and xyloglucan endotransglucosylase/hydrolase increased with salt and decreased with additional PDS solution. Furthermore, glycoproteins decreased with salt stress and recovered with additional treatment. As mitochondrion-related events, the contents of ATP and gamma-aminobutyric acid increased with salt stress and recovered with additional treatment. These results suggest that PDS solution improves the soybean growth by alleviating salt stress. Additionally, the regulation of energy metabolism, protein glycosylation, and cell wall construction might be an important factor for the acquisition of salt tolerance in soybean.

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“…After additional incubation, the absorbance was measured at 555 nm. The contents of gamma-aminobutyric acid were determined with reference to the standard curve [ 67 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Measurement Of Gamma-aminobutyric Acid Contentsmentioning

confidence: 99%

Proteomic Analysis Reveals Salt-Tolerant Mechanism in Soybean Applied with Plant-Derived Smoke Solution

Komatsu,

Kimura,

Rehman

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…For measurement, 0.5 mL of solution I was added to 50 µL of sample solution containing GABA and incubated for 10 min at 30 • C. After incubation, 0.5 mL of solution II was added and incubation was continued for 20 min at 30 • C. After incubation, each assay was measured at absorbance at 555 nm. GABA content was determined by referring to the calibration curves [49].…”

Section: Assay Of the Contents Of Pyruvic Acid Gaba Glutamic Acid And...mentioning

confidence: 99%

The Changes of Amino-Acid Metabolism between Wheat and Rice during Early Growth under Flooding Stress

Komatsu,

Egishi,

Ohno

2024

IJMS

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Floods induce hypoxic stress and reduce wheat growth. On the other hand, rice is a semi-aquatic plant and usually grows even when partially submerged. To clarify the dynamic differences in the cellular mechanism between rice and wheat under flooding stress, morphological and biochemical analyses were performed. Although the growth of wheat in the early stage was significantly suppressed due to flooding stress, rice was hardly affected. Amino-acid analysis revealed significant changes in amino acids involved in the gamma-aminobutyric acid (GABA) shunt and anaerobic/aerobic metabolism. Flood stress significantly increased the contents of GABA and glutamate in wheat compared with rice, though the abundances of glutamate decarboxylase and succinyl semialdehyde dehydrogenase did not change. The abundance of alcohol dehydrogenase and pyruvate carboxylase increased in wheat and rice, respectively. The contents of aspartic acid and pyruvic acid increased in rice root but remained unchanged in wheat; however, the abundance of aspartate aminotransferase increased in wheat root. These results suggest that flooding stress significantly inhibits wheat growth through upregulating amino-acid metabolism and increasing the alcohol-fermentation system compared to rice. When plant growth is inhibited by flooding stress and the aerobic-metabolic system is activated, GABA content increases.

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