G6PD enzyme activity in normal term neonates and adults using OSMMR-D kit with HB normalisation

Increased basal oxidation of peroxiredoxin 2 and limited peroxiredoxin recycling in glucose‐6‐phosphate dehydrogenase‐deficient erythrocytes from newborn infants

“…The reference values for normal, partial deficiency, and deficiency of G6PD activity were >6.76, 2.25–6.76, and <2.25 U/g hemoglobin, respectively, adapted based on the ranges published by Azma et al . (23).…”

“…Quantitative measurement of G6PD enzyme activity was performed on 5 l of whole EDTA blood using the OSMMR-D G6PD assay kit (R & D Diagnostics, Holargos, Greece) with hemoglobin normalization, according to the supplier's instructions. The reference values for normal, partial deficiency, and deficiency of G6PD activity were Ͼ6.76, 2.25-6.76, and Ͻ2.25 U/g hemoglobin, respectively, adapted based on the ranges published by Azma et al (23).…”

Evaluation of glucose-6-phosphate dehydrogenase stability in stored blood samples

“…The G6PD activity assay was done daily for seven consecutive days during which the EDTA cord blood, stored at 2-8 °C and the air-dried blood on chromatography paper, stored in a dark place at room temperature. G6PD assays were performed using OSMMR-D G6PD assay kit with haemoglobin normalization from R and D Diagnostics (Holargos, Greece) and enzyme activity measured on a spectrophotometer (Ultramicroplate Reader EL808, Bio Tek, Instruments) as previously described (Azma et al, 2010[ 4 ]). For the batch of control samples provided by the manufacrurer, a reference range of G6PD activity for the newborns was established as recommended (Azma et al, 2010[ 4 ]).…”

“…G6PD assays were performed using OSMMR-D G6PD assay kit with haemoglobin normalization from R and D Diagnostics (Holargos, Greece) and enzyme activity measured on a spectrophotometer (Ultramicroplate Reader EL808, Bio Tek, Instruments) as previously described (Azma et al, 2010[ 4 ]). For the batch of control samples provided by the manufacrurer, a reference range of G6PD activity for the newborns was established as recommended (Azma et al, 2010[ 4 ]). The mean G6PD activity for the newborns with normal G6PD activity was 12.8 U/g Hb.…”

“…A national programme of mandatory screening for G6PD deficiency of all newborns has been instituted since early 1980s, employing the WHO recommended semiquantitative Fluorescent Spot Test (FST) because of the association with severe neonatal jaundice (Beutler and Mitchell, 1968[ 6 ]). However, the lack of sensitivity of the FST to detect individuals with moderate enzyme deficiency (activity > 20 % < 60 % of normal mean) (Reclos et al, 2000[ 12 ]; Minucci et al, 2008[ 10 ], Ainoon et al, 2003[ 1 ]) who are also at risk for severe haemolysis, has led us to introduce the quantitative G6PD enzyme assay method at our centre for routine screening of newborns (Ainoon et al, 2003[ 1 ]; Azma et al, 2010[ 4 ]). Being the sole hospital to offer G6PD assay as a service our center has become a referral laboratory and have been receiving a substantial number of samples from other hospitals including state hospitals.…”