G4-Квадруплексы И Геномная Нестабильность

Зыбайлов, Б. Л.; Sherpa, M. D.; Глазко, Г. В.; Raney, K. D.; Глазко, В. И.

doi:10.7868/s0026898413020183

Cited by 4 publications

(3 citation statements)

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“…In this study, we aimed to clarify whether the basic internal features of quadruplex units predetermine multimerization in a condition-dependent manner and analysed blunt-ended G4 motifs, each comprising four G3/G2 tracks interrupted by short or relatively short non-G tracks (loops). Such motifs, particularly those with single-nucleotide loops, are abundant in the human genome ( 37 , 38 ) and are often colocalized with chromosomal fragile sites ( 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…Genomic G4s are believed to play important roles in several biological processes, such as the regulation of telomere maintenance, transcription, replication and translation ( 2 , 3 ). Their relevance for genomic instability (non-homologous recombination and other rearrangements) has also been widely discussed ( 4 ), and elucidation of the interaction patterns of G4-prone DNA fragments may provide insight into instability-related processes.…”

Section: Introductionmentioning

confidence: 99%

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Polymorphism of G4 associates: from stacks to wires via interlocks

Varizhuk

Protopopova

Цветков

et al. 2018

Nucleic Acids Research

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We examined the assembly of DNA G-quadruplexes (G4s) into higher-order structures using atomic force microscopy, optical and electrophoretic methods, NMR spectroscopy and molecular modeling. Our results suggest that parallel blunt-ended G4s with single-nucleotide or modified loops may form different types of multimers, ranging from stacks of intramolecular structures and/or interlocked dimers and trimers to wires. Decreasing the annealing rate and increasing salt or oligonucleotide concentrations shifted the equilibrium from intramolecular G4s to higher-order structures. Control antiparallel and hybrid G4s demonstrated no polymorphism or aggregation in our experiments. The modification that mimics abasic sites (1′,2′-dideoxyribose residues) in loops enhanced the oligomerization/multimerization of both the 2-tetrad and 3-tetrad G4 motifs. Our results shed light on the rules that govern G4 rearrangements. Gaining control over G4 folding enables the harnessing of the full potential of such structures for guided assembly of supramolecular DNA structures for nanotechnology.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polymorphism of G4 associates: from stacks to wires via interlocks

Varizhuk

Protopopova

Цветков

et al. 2018

Nucleic Acids Research

View full text Add to dashboard Cite

show abstract

“…A total of 80–90 potential quadruplex-forming sequences have been found throughout the human mitochondrial genome, strongly biased towards the heavy strand of DNA, which has a significantly high content of guanine nucleotides. Yeast mtDNA contains ∼0.373 potential quadruplex-forming sequences per 1000 base pairs, which is an order of magnitude larger than in nuclear DNA (∼0.034–0.067) [159,160]. Interestingly, some studies suggest that, along with other helix-distorting and intrinsically curved regions of DNA, G4s may be a significant cause of DNA instability within the mitochondrial genome [161].…”

Section: Mitochondrial Dna Replication — Copying the Circlementioning

confidence: 99%

Mitochondrial DNA replication: a PrimPol perspective

Bailey

Doherty

2017

Biochemical Society Transactions

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PrimPol, (primase–polymerase), the most recently identified eukaryotic polymerase, has roles in both nuclear and mitochondrial DNA maintenance. PrimPol is capable of acting as a DNA polymerase, with the ability to extend primers and also bypass a variety of oxidative and photolesions. In addition, PrimPol also functions as a primase, catalysing the preferential formation of DNA primers in a zinc finger-dependent manner. Although PrimPol's catalytic activities have been uncovered in vitro, we still know little about how and why it is targeted to the mitochondrion and what its key roles are in the maintenance of this multicopy DNA molecule. Unlike nuclear DNA, the mammalian mitochondrial genome is circular and the organelle has many unique proteins essential for its maintenance, presenting a differing environment within which PrimPol must function. Here, we discuss what is currently known about the mechanisms of DNA replication in the mitochondrion, the proteins that carry out these processes and how PrimPol is likely to be involved in assisting this vital cellular process.

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Structure, properties, and biological relevance of the DNA and RNA G-quadruplexes: Overview 50 years after their discovery

Dolinnaya

Ogloblina²,

Yakubovskaya³

2016

Biochemistry Moscow

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G-quadruplexes (G4s), which are known to have important roles in regulation of key biological processes in both normal and pathological cells, are the most actively studied non-canonical structures of nucleic acids. In this review, we summarize the results of studies published in recent years that change significantly scientific views on various aspects of our understanding of quadruplexes. Modern notions on the polymorphism of DNA quadruplexes, on factors affecting thermodynamics and kinetics of G4 folding-unfolding, on structural organization of multiquadruplex systems, and on conformational features of RNA G4s and hybrid DNA-RNA G4s are discussed. Here we report the data on location of G4 sequence motifs in the genomes of eukaryotes, bacteria, and viruses, characterize G4-specific small-molecule ligands and proteins, as well as the mechanisms of their interactions with quadruplexes. New information on the structure and stability of G4s in telomeric DNA and oncogene promoters is discussed as well as proof being provided on the occurrence of G-quadruplexes in cells. Prominence is given to novel experimental techniques (single molecule manipulations, optical and magnetic tweezers, original chemical approaches, G4 detection in situ, in-cell NMR spectroscopy) that facilitate breakthroughs in the investigation of the structure and functions of G-quadruplexes.

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G4-Квадруплексы И Геномная Нестабильность

Cited by 4 publications

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Polymorphism of G4 associates: from stacks to wires via interlocks

Polymorphism of G4 associates: from stacks to wires via interlocks

Mitochondrial DNA replication: a PrimPol perspective

Structure, properties, and biological relevance of the DNA and RNA G-quadruplexes: Overview 50 years after their discovery

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