G-quadruplexes for luminescent sensing and logic gates

He, Hong‐Zhang; Chan, Daniel Shiu‐Hin; Leung, Chung‐Hang; Ma, Dik‐Lung

doi:10.1093/nar/gkt108

Cited by 152 publications

(95 citation statements)

References 82 publications

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“…[3][4][5][6][7][8][9][10] Because their formation is mostly related to the processes of transcription, replication, and recombination, they are highly associated with human diseases 11 (e.g., cancer, 12,13 cardiovascular diseases 14 and diabetes 15 ). [35][36][37][38] Up to now, carbazole and its derivatives have been widely utilized as functional building blocks in the preparation of organic medicine, pesticides, materials, etc., because of their excellent solubility, stability, large π-conjugation system, and special rigid structure, and because they can be easily functionalized at 3, 6 and 9-positions and covalently linked with other molecular groups, and have excellent and biological activity. [16][17][18] Many G4 ligands have been designed and synthesized, containing a planar aromatic core, that bind to G4s through π-π stacking interactions and exhibit absorption or fluorescence.…”

Section: Introductionmentioning

confidence: 99%

A benzindole substituted carbazole cyanine dye: a novel targeting fluorescent probe for parallel c-myc G-quadruplexes

Lin

Fei

et al. 2015

Analyst

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Many organic ligands were synthesized to recognize G-quadruplexes. However, different kinds of G-quadruplexes (G4s) possess different structures and functions. Therefore, selective recognition of certain types of G4s is important for the study of G4s. In this paper, a novel cyanine dye, 3-(2-(4-vinylpyridine))-6-(2-((1-(4-sulfobutyl))-3,3-dimethyl-2-vinylbenz[e]indole)-9-ethyl-carbazole (9E PBIC), composed of benzindole and carbazole was designed and synthesised. The studies on UV-vis and fluorescence properties of the dye with different DNA forms showed that the dye exhibits almost no fluorescence under aqueous buffer conditions, but it increased over 100 fold in the presence of c-myc G4 and 10-30 fold in the presence of other G4s, while little in the presence of single/double-stranded DNA, indicating that it has excellent selectivity to c-myc 2345 G4. For the binding studies the dye is interacted with the c-myc 2345 G-quadruplex by using the end-stack binding model. It can be said that the dye is an excellent targeting fluorescent probe for c-myc G-quadruplexes.

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Section: Introductionmentioning

confidence: 99%

A benzindole substituted carbazole cyanine dye: a novel targeting fluorescent probe for parallel c-myc G-quadruplexes

Lin

Fei

et al. 2015

Analyst

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“…The released single strand DNA S 6 will be a new primer to hybridize with circular template (CT) and initiate rolling circle replication and produce single long strand DNA with the help of phi29 DNA polymerase. There are many guanine bases in the single long strand DNA strands and G-quadruplex structure will be formed with potassium ions as stabilizer2829.…”

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confidence: 99%

Label-free detection of microRNA based on coupling multiple isothermal amplification techniques

Zheng

Niu

Wei

et al. 2016

Sci Rep

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MicroRNA (miRNA) was a promising class of cancer biomarkers. Here we developed a label-free method for sensitive measurement of let-7d miRNA based on multiple amplification techniques. The primer will bind to the duplex strand DNA that was formed by stem-loop template and target let-7d to initiate strand displacement amplification (SDA) in tandem. The released single strand DNA will be a primer to bind the circular template to initiate rolling circle amplification (RCA). The products based on multiple amplifications will be detected by a standard fluorimeter with N-methyl mesoporphyrin IX (NMM) as the fluorescent indicator. The proposed method exhibited excellent selectivity and high sensitivity with a detection limit of as low as 1.5 × 10−13 M. Moreover, this methodology was used for the determination of biomolecules in real serum samples with satisfying results.

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“…[14][15][16][17][18][19][20][21][22][23] For example, Qu's group developed a label-free fluorescence turn-on method based on the unique strong interaction between N-methyl mesoporphyrin IX and the folded G-quadruplex. [28][29][30][31] More recently, Liu and Chen, have also developed a novel nicking enzyme assisted signal amplification method based on G-quadruplex structures for sensitive label-free colorimetric analysis of UDG activity. [24][25][26] The double quadruplex DNA has also been found applications in biology and nanotechnology under specific conditions; 27 however, the G-quadruplex DNA represents a more versatile sensing platform for bioanalytical assays because the specific recognition can be done by luminescent sensing probes.…”

Section: Introductionmentioning

confidence: 99%

Sensitive and selective detection of uracil-DNA glycosylase activity with a new pyridinium luminescent switch-on molecular probe

Deng

et al. 2015

Analyst

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Uracil-deoxyribonucleic acid glycosylase (UDG) is known to function as an important base-excision repair enzyme and eliminate uracil from DNA molecules to maintain genomic integrity. A new small organic molecule (DID-VP) with interesting structural properties was synthesized as a G-quadruplex selective ligand and was demonstrated to be a sensitive luminescent switch-on probe in a convenient luminescent assay specifically for UDG detection in fetal bovine serum samples under rapid and simple conditions. This newly developed analytical method is based on the UDG enzymatic activity to unwind a duplex DNA substrate, and comprises a G-quadruplex-forming sequence (ON1) and uracil-containing DNA strand (ON2) to generate a remarkable fluorescence signal through the specific interaction of DID-VP with ON1. This luminescent switch-on assay is able to achieve high sensitivity and specificity for UDG over other enzymes. The application range of the present analytical system is found to be 0.05 to 1.00 U mL(-1) UDG with a very low detection limit of 0.005 U mL(-1). The recovery study of UDG in real samples gave a very good performance with 75.05%-102.7% recovery. In addition, an extended application of the assay in screening of UDG inhibitors is demonstrated. A good dose-dependence of the luminescence response with respect to the concentration of UDG inhibitors in samples was observed.

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G-quadruplexes for luminescent sensing and logic gates

Cited by 152 publications

References 82 publications

A benzindole substituted carbazole cyanine dye: a novel targeting fluorescent probe for parallel c-myc G-quadruplexes

A benzindole substituted carbazole cyanine dye: a novel targeting fluorescent probe for parallel c-myc G-quadruplexes

Label-free detection of microRNA based on coupling multiple isothermal amplification techniques

Sensitive and selective detection of uracil-DNA glycosylase activity with a new pyridinium luminescent switch-on molecular probe

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