G-quadruplex DNA aptamers generated for systemin

Bing, Tao; Chang, Tianjun; Xiao, Yang; Mei, He; Liu, Xiangjun; Shangguan, Dihua

doi:10.1016/j.bmc.2011.05.061

Cited by 25 publications

(24 citation statements)

References 38 publications

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“…Increasing evidence support biological roles for these oddities, [1,2] which may also find application in nanotechnologies [3] and biotechnologies (DNAzymes, aptamers…). [4,5] DNA quadruplexes are highly polymorphic: intramolecular structures may be parallel, antiparallel or "3+1" (three strands pointing in the same direction whereas the fourth one has the opposite polarity) although tetramolecular DNA quadruplexes tend to be all parallel. Tetramolecular G4 structures are widely used as simple models of quadruplex motifs.…”

Section: Introductionmentioning

confidence: 99%

Orienting Tetramolecular G‐Quadruplex Formation: The Quest for the Elusive RNA Antiparallel Quadruplex

Mendoza

Porrini

Salgado

et al. 2015

Chemistry A European J

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DNA and RNA G-quadruplexes (G4) are unusual nucleic acid structures involved in a number of key biological processes. RNA G-quadruplexes are less studied although recent evidence demonstrates that they are biologically relevant. Compared to DNA quadruplexes, RNA G4 are generally more stable and less polymorphic. Duplexes and quadruplexes may be combined to obtain pure tetrameric species. Here, we investigated whether classical antiparallel duplexes can drive the formation of antiparallel tetramolecular quadruplexes. This concept was first successfully applied to DNA G4. In contrast, RNA G4 were found to be much more unwilling to adopt the forced antiparallel orientation, highlighting that the reason RNA adopts a different structure must not be sought in the loops but in the G-stem structure itself. RNA antiparallel G4 formation is likely to be restricted to a very small set of peculiar sequences, in which other structural features overcome the formidable intrinsic barrier preventing its formation.

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Section: Introductionmentioning

confidence: 99%

Orienting Tetramolecular G‐Quadruplex Formation: The Quest for the Elusive RNA Antiparallel Quadruplex

Mendoza

Porrini

Salgado

et al. 2015

Chemistry A European J

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“…In order to have a better perception into the interaction between aptamer and CAP, the effect of ionic strength on aptamer binding to CAP was examined with different concentrations of K + . Moreover, K + ions might influence the configuration of the aptamers that contain G‐quadruplex structure 28. As anticipated, low ionic concentration did not affect aptamer binding to CAP and the binding was strong.…”

Section: Resultsmentioning

confidence: 71%

Label‐Free Electrochemical Aptasensor for Determination of Chloramphenicol Based on Gold Nanocubes‐Modified Screen‐Printed Gold Electrode

et al. 2015

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An ultrasensitive label‐free electrochemical aptasensor was developed for selective detection of chloramphenicol (CAP). The aptasensor was made using screen‐printed gold electrode modified with synthesized gold nanocube/cysteine. The interactions of CAP with aptamer were studied by cyclic voltammetry, square wave voltammetry (SWV) and electrochemical impedance spectroscopy. Under optimized conditions, two linear calibration curves were obtained for CAP determination using SWV technique, from 0.03 to 0.10 µM and 0.25–6.0 µM with a detection limit of 4.0 nM. The aptasensor has the advantages of good selectivity and stability and applied to the determination of CAP in human blood serum sample.

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“…The sequence truncation of aptamer may be carried out many times to find out the minimized sequence. Based on the secondary structure and binding motifs, the minimized aptamer sequences can be further optimized to obtain better binding affinity by removing, adding, and/or displacing one or several nucleotides [31,32,[38][39][40][41].…”

Section: Characterization Of Aptamersmentioning

confidence: 99%

Cell-SELEX: Aptamer Selection Against Whole Cells

Shangguan

Bing

Zhang

2015

Aptamers Selected by Cell-Selex for Theranostics

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Changes at the molecular level always occur at different stages in diseased cells. The detection of these changes is critical for understanding the molecular mechanisms underlying pathogenesis, as well as accurately diagnosing disease states and monitoring therapeutic modalities. Cell-SELEX is a foundational tool used to select probes able to recognize molecular signatures on the surface of diseased cells. This technology has been increasingly used in biomarker discovery, as well as cancer diagnosis and therapy. In this chapter, the whole cell-SELEX process is described, including aptamer selection, identification, and validation. In addition, we will explore the challenges and prospects for cell-SELEX now and in the coming years. It is anticipated that this chapter will guide readers toward a better understanding of the working principles underlying the cell-SELEX technology and serve as a practical reference for bench scientists engaged in cell and molecular biology.

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G-quadruplex DNA aptamers generated for systemin

Cited by 25 publications

References 38 publications

Orienting Tetramolecular G‐Quadruplex Formation: The Quest for the Elusive RNA Antiparallel Quadruplex

Orienting Tetramolecular G‐Quadruplex Formation: The Quest for the Elusive RNA Antiparallel Quadruplex

Label‐Free Electrochemical Aptasensor for Determination of Chloramphenicol Based on Gold Nanocubes‐Modified Screen‐Printed Gold Electrode

Cell-SELEX: Aptamer Selection Against Whole Cells

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