G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection

Nie, Ji; Zhang, Dewen; Tie, Cai; Zhou, Ying‐Lin; Zhang, Xin‐Xiang

doi:10.1016/j.bios.2014.01.032

Cited by 48 publications

(23 citation statements)

References 23 publications

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“…The detection limit was 0.08 pM. This detection limit was not only much lower than other assays for the detection of target DNA with different measurement protocols such as electrochemistry (Wan et al, 2014;Cui et al, 2014;Dharuman and Hahn, 2008), fluorimetry Sun et al, 2014;Sharon et al, 2010;Zhang et al, 2013), colorimetry (Nie et al, 2014), but also much lower than those PEC detection with different amplification strategies (Golub et al, 2012;Liu et al, 2006Liu et al, , 2008. The detailed comparison for different methods is listed in Table S2.…”

Section: Pec Detection Of Dnamentioning

confidence: 96%

A novel photoelectrochemical sensor based on photocathode of PbS quantum dots utilizing catalase mimetics of bio-bar-coded platinum nanoparticles/G-quadruplex/hemin for signal amplification

Wang

Liu

Shu

et al. 2015

Biosensors and Bioelectronics

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Section: Pec Detection Of Dnamentioning

confidence: 96%

A novel photoelectrochemical sensor based on photocathode of PbS quantum dots utilizing catalase mimetics of bio-bar-coded platinum nanoparticles/G-quadruplex/hemin for signal amplification

Wang

Liu

Shu

et al. 2015

Biosensors and Bioelectronics

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“…Zhang and co-workers developed at wo-template system (X'-X' and Y'-X')t hat generated horseradish peroxidase (HRP) mimicking DNAzymes to generate ac olor change. [65] As ingle-template EXPAR system was also used to generate aDNAzyme-catalyzed color change. [66] Thetemplate initially adopted ah airpin structure until it was opened by at rigger sequence that hybridized to the 5'-end of the template.Along primer oligo then hybridized to the 3'-end of the template and triggered EXPAR.…”

Section: Colorimetric Detectionmentioning

confidence: 99%

Exponential Isothermal Amplification of Nucleic Acids and Assays for Proteins, Cells, Small Molecules, and Enzyme Activities: An EXPAR Example

2018

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Isothermal exponential amplification techniques, such as strand-displacement amplification (SDA), rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), nucleic acid sequence based amplification (NASBA), helicase-dependent amplification (HDA), and recombinase polymerase amplification (RPA), have great potential for on-site, point-of-care, and in situ assay applications. These amplification techniques eliminate the need for temperature cycling, as required for the polymerase chain reaction (PCR), while achieving comparable amplification yields. We highlight here recent advances in the exponential amplification reaction (EXPAR) for the detection of nucleic acids, proteins, enzyme activities, cells, and metal ions. The incorporation of fluorescence, colorimetric, chemiluminescence, Raman, and electrochemical approaches enables the highly sensitive detection of a variety of targets. Remaining issues, such as undesirable background amplification resulting from nonspecific template interactions, must be addressed to further improve isothermal and exponential amplification techniques.

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“…This heating also enabled Pardee et al to detect Zika virus RNA in serum with high sensitivity using NASBA [21]. HCR performs especially well in serum without any pretreatment [28,119,[133][134][135], presumably because the reaction relies on cascaded hybridization events instead of polymerases.…”

Section: Direct Naats For Plasma and Serummentioning

confidence: 99%

Advances in Directly Amplifying Nucleic Acids from Complex Samples

Walker

Hsieh

2019

Biosensors

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Advances in nucleic acid amplification technologies have revolutionized diagnostics for systemic, inherited, and infectious diseases. Current assays and platforms, however, often require lengthy experimental procedures and multiple instruments to remove contaminants and inhibitors from clinically-relevant, complex samples. This requirement of sample preparation has been a bottleneck for using nucleic acid amplification tests (NAATs) at the point of care (POC), though advances in "lab-on-chip" platforms that integrate sample preparation and NAATs have made great strides in this space. Alternatively, direct NAATs-techniques that minimize or even bypass sample preparation-present promising strategies for developing POC diagnostic tools for analyzing real-world samples. In this review, we discuss the current status of direct NAATs. Specifically, we surveyed potential testing systems published from 1989 to 2017, and analyzed their performances in terms of robustness, sensitivity, clinical relevance, and suitability for POC diagnostics. We introduce bubble plots to facilitate our analysis, as bubble plots enable effective visualization of the performances of these direct NAATs. Through our review, we hope to initiate an in-depth examination of direct NAATs and their potential for realizing POC diagnostics, and ultimately transformative technologies that can further enhance healthcare.

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G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection

Cited by 48 publications

References 23 publications

A novel photoelectrochemical sensor based on photocathode of PbS quantum dots utilizing catalase mimetics of bio-bar-coded platinum nanoparticles/G-quadruplex/hemin for signal amplification

A novel photoelectrochemical sensor based on photocathode of PbS quantum dots utilizing catalase mimetics of bio-bar-coded platinum nanoparticles/G-quadruplex/hemin for signal amplification

Exponential Isothermal Amplification of Nucleic Acids and Assays for Proteins, Cells, Small Molecules, and Enzyme Activities: An EXPAR Example

Advances in Directly Amplifying Nucleic Acids from Complex Samples

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