G-quadruplex-based assay combined with aptamer and gold nanoparticles for Escherichia coli K88 determination

Wang, Zefeng; Lu, Qiujun; Xu, Tao; Wang, Feiying; Huang, Fangfang; Peng, Yanling; Deng, Le

doi:10.1007/s00604-020-04291-x

Cited by 13 publications

(4 citation statements)

References 33 publications

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“…In addition, this colorimetric biosensor could realize accurate detection of MRSA in human body fluids. Deng et al presented a colorimetric method for E. coli K88 (ETEC K88) detection based on a G-quadruplex and DNA-functionalized gold nanoparticles (AuNPs) [65]. The AuNPs were firstly modified by capturing DNA.…”

Section: Aptamer-functionalized Gold Nanoparticlesmentioning

confidence: 99%

Recent Advances in Colorimetric Sensors Based on Gold Nanoparticles for Pathogen Detection

et al. 2022

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Infectious pathogens cause severe threats to public health due to their frightening infectivity and lethal capacity. Rapid and accurate detection of pathogens is of great significance for preventing their infection. Gold nanoparticles have drawn considerable attention in colorimetric biosensing during the past decades due to their unique physicochemical properties. Colorimetric diagnosis platforms based on functionalized AuNPs are emerging as a promising pathogen-analysis technique with the merits of high sensitivity, low-cost, and easy operation. This review summarizes the recent development in this field. We first introduce the significance of detecting pathogens and the characteristics of gold nanoparticles. Four types of colorimetric strategies, including the application of indirect target-mediated aggregation, chromogenic substrate-mediated catalytic activity, point-of-care testing (POCT) devices, and machine learning-assisted colorimetric sensor arrays, are systematically introduced. In particular, three biomolecule-functionalized AuNP-based colorimetric sensors are described in detail. Finally, we conclude by presenting our subjective views on the present challenges and some appropriate suggestions for future research directions of colorimetric sensors.

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Section: Aptamer-functionalized Gold Nanoparticlesmentioning

confidence: 99%

Recent Advances in Colorimetric Sensors Based on Gold Nanoparticles for Pathogen Detection

et al. 2022

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“…G-quadruplex also contributes high specificity towards target molecules [50]. G-quadruplex based-detection shows a promising trend for selective determination of heavy metal ions [51,52,53] and pathogens [54,55]. Guanine rich oligonucleotides favor detection of Pb 2+ by forming a G-quadruplex structure with high specificity.…”

Section: Pb 2+ Detectionmentioning

confidence: 99%

Aptamer functionalized ZnO thin-film transistor based multiplexed detection of Lead and E. coli in water

Honnali

Ragha

Ashwath

et al. 2022

Preprint

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Background: Contamination of lead (Pb 2+) disturbs biological functions and causes neurotoxicity even at low levels. Pathogens such as Escherichia coli, P. aeruginosa and S. aureus found in packaged drinking water causes infections. Therefore, we demonstrate the multiplexed detection of Pb 2+ and E. coli in water using ZnO thin-film transistor (TFT) arrays. Results: Low voltage ZnO TFTs fabricated by physical vapour deposition were functionalized with APTES-glutaraldehyde molecules. TFTs with such functionalized ZnO surfaces showed good sensitivity and high specificity to the targets. The TFTs showed a detection limit of 27 nM and 10 5 cfu/ml for Pb 2+ and E. coli, respectively with the TFT flat-band voltage (V fb), and hence the TFT transconductance, being dependent on the target concentration. The direct integration of the sensing with the TFT and associated integrated circuits promises high density sensor arrays.Conclusion: The present work highlights the importance of aptamers in the multiplexed detection of different targets using low cost ZnO TFT array.

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“…In case of presence of common intestinal pathogen E. coli K88 in the test sample, aptamer leaves capture probe-AuNPs and preferably forms complex with E. coli . The G-quadruplex binds to capture DNA-AuNPs to induce the aggregation and color change of AuNPs [ 26 •]. AuNP-based DNA nanosensors are most commonly used for microorganism detection (Fig.…”

Section: Detection Of Pathogensmentioning

confidence: 99%

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

Kumar

Guleria

2020

Curr Pollution Rep

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G-quadruplex-based assay combined with aptamer and gold nanoparticles for Escherichia coli K88 determination

Cited by 13 publications

References 33 publications

Recent Advances in Colorimetric Sensors Based on Gold Nanoparticles for Pathogen Detection

Recent Advances in Colorimetric Sensors Based on Gold Nanoparticles for Pathogen Detection

Aptamer functionalized ZnO thin-film transistor based multiplexed detection of Lead and E. coli in water

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

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