G-protein gamma subunit 1 is required for sugar reception in Drosophila

Ishimoto, Hiroshi; Takahashi, Kuniaki; Ueda, Ryo; Tanimura, Teiichi

doi:10.1038/sj.emboj.7600796

Cited by 44 publications

(41 citation statements)

References 37 publications

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“…2 B), and the behavioral and electrophysiological responses to water were not different among all DGs␣ strains examined in this study. It is known that salt responses in larvae require amiloride-sensitive channels encoded by ppk11 and ppk19 (Liu et al, 2003), and the low and high concentrations of salt responses do not require G␥1 in adult flies (Ishimoto et al, 2005). These findings together with our results suggest that DGs␣ in non-Gr5a GRNs serves for other signaling than taste or that the non-Gr5a GRNs containing DGs␣ are mechanosensory neurons.…”

Section: Discussionsupporting

confidence: 76%

“…Recently, a ␥ subunit of the G-protein encoded by G␥1 was reported to be required for sugar perception (Ishimoto et al, 2005). Although this finding confirms the hypothesis that the sugargustatory receptors, including Gr5a, are coupled to G-proteins, it does not specify the downstream pathway for sugar-taste signaling in Drosophila.…”

Section: Discussionsupporting

confidence: 33%

“…In the Drosophila genome, 11 genes encode G␣ (Ishimoto et al, 2005), and one of them, DGs␣, is a homolog of mammalian Gs (Quan et al, 1989). The primary function of the Gs family is to elevate the concentration of cAMP via adenylyl cyclase (AC), and in vertebrates, this transduction pathway is involved in a variety of cellular functions (Tesmer and Sprang, 1998;Hurley, 1999;Simonds, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Gs Is Involved in Sugar Perception in Drosophila melanogaster

Ueno

Kohatsu

Clay

et al. 2006

Journal of Neuroscience

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In Drosophila melanogaster, gustatory receptor genes (Grs) encode G-protein-coupled receptors (GPCRs) in gustatory receptor neurons (GRNs) and some olfactory receptor neurons. One of the Gr genes, Gr5a, encodes a sugar receptor that is expressed in a subset of GRNs and has been most extensively studied both molecularly and physiologically, but the G-protein ␣ subunit (G␣) that is coupled to this sugar receptor remains unknown. Here, we propose that Gs is the G␣ that is responsible for Gr5a-mediated sugar-taste transduction, based on the following findings: First, immunoreactivities against Gs were detected in a subset of GRNs including all Gr5a-expressing neurons. Second, trehalose-intake is reduced in flies heterozygous for null mutations in DGs␣, a homolog of mammalian Gs, and trehalose-induced electrical activities in sugar-sensitive GRNs were depressed in those flies. Furthermore, expression of wild-type DGs␣ in sugar-sensitive GRNs in heterozygotic DGs␣ mutant flies rescued those impairments. Third, expression of double-stranded RNA for DGs␣ in sugarsensitive GRNs depressed both behavioral and electrophysiological responses to trehalose. Together, these findings indicate that DGs␣ is involved in trehalose perception. We suggest that sugar-taste signals are processed through the Gs␣-mediating signal transduction pathway in sugar-sensitive GRNs in Drosophila.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionsupporting

confidence: 33%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gs Is Involved in Sugar Perception in Drosophila melanogaster

Ueno

Kohatsu

Clay

et al. 2006

Journal of Neuroscience

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“…For example, it seems quite reasonable to assume that all GRs act though the same set of signaling molecules, albeit separate pathways for different taste modalities cannot be ruled out. To date, only two G-protein subunits, Gsα and Gγ, have been implicated in the GR pathway, and both subunits are required for sugar detection [90,91]. The Drosophila Gs homolog, DGsα was recently shown to be expressed in approximately half of the GRNs in the labellum using specific antibodies, suggesting a role in gustatory signal transduction [91].…”

Section: Signal Transductionmentioning

confidence: 99%

“…The G-protein subunit, Gγ1, is expressed in the GRNs of the labellum [90]. Flies defective for Gγ1 show reduced behavioral response to sucrose, glucose, fructose, and trehalose, indicating that Gγ1 is involved in the signal transduction of sugar taste perception.…”

Section: Signal Transductionmentioning

confidence: 99%

Taste and pheromone perception in the fruit fly Drosophila melanogaster

Ebbs

Amrein

2007

Pflugers Arch - Eur J Physiol

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Taste is an essential sense for detection of nutrient-rich food and avoidance of toxic substances. The Drosophila melanogaster gustatory system provides an excellent model to study taste perception and taste-elicited behaviors. "The fly" is unique in the animal kingdom with regard to available experimental tools, which include a wide repertoire of molecular-genetic analyses (i.e., efficient production of transgenics and gene knockouts), elegant behavioral assays, and the possibility to conduct electrophysiological investigations. In addition, fruit flies, like humans, recognize sugars as a food source, but avoid bitter tasting substances that are often toxic to insects and mammals alike. This paper will present recent research progress in the field of taste and contact pheromone perception in the fruit fly. First, we shall describe the anatomical properties of the Drosophila gustatory system and survey the family of taste receptors to provide an appropriate background. We shall then review taste and pheromone perception mainly from a molecular genetic perspective that includes behavioral, electrophysiological and imaging analyses of wild type flies and flies with genetically manipulated taste cells. Finally, we shall provide an outlook of taste research in this elegant model system for the next few years.Keywords Drosophila . Gustatory receptor neurons . GTP-binding protein-coupled receptors Like most animals, Drosophila monitor their chemical world with two distinct senses: the olfactory system, which is used for the detection of volatile chemicals, and the gustatory (taste) system, which enables the fly to detect soluble compounds. The olfactory sensory system (or the fly nose) is comprised of two pairwise head appendages, the third segments of the antennae, and the maxillary palps (Fig. 1). These appendages are covered with hundreds of sensory hairs, each of which contains two to four olfactory sensory neurons (OSNs) [1]. In contrast, the gustatory system is widely distributed over the animal's body. The main taste organ, the proboscis or labial palps (i.e., the fly tongue), is located on the distal end of the labellum (also a head appendage), but flies, like many other insects, have taste sensilla on legs and wings and, in females, on the genitalia [1,2].The roles of the olfactory and taste systems are clearly separated with regard to the physical state of chemicals they detect (volatile vs solubilized). However, the two systems often cooperate to fulfill specific functions in related processes and behaviors, the most obvious of them being the location and identification of food. For example, chemical cues such as the specific odor of a flower and the sugar compounds present in its nectar are by nature associated with one another. Thus, the odor cue provides a primary sensory input for an insect such as a honeybee to locate a food source (nectar), whose particular chemical composition is subsequently verified by the taste system. Similarly, the typical odor of yeast, which produces high amounts of t...

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Taste Processing in Insects

Glendinning

2015

Handbook of Olfaction and Gustation

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G-protein gamma subunit 1 is required for sugar reception in Drosophila

Cited by 44 publications

References 37 publications

Gs Is Involved in Sugar Perception in Drosophila melanogaster

Gs Is Involved in Sugar Perception in Drosophila melanogaster

Taste and pheromone perception in the fruit fly Drosophila melanogaster

Taste Processing in Insects

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