G-protein coupling and nuclear translocation of the human abscisic acid receptor LANCL2

Fresia, Chiara; Vigliarolo, Tiziana; Guida, Lucrezia; Booz, Valeria; Bruzzone, Santina; Sturla, Laura; Bona, Melody Di; Pesce, Mattia; Usai, Cesare; Flora, Antonio De; Zocchi, Elena

doi:10.1038/srep26658

Cited by 39 publications

(45 citation statements)

References 56 publications

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“…In contrast, the level of HSP70 transcripts increased in 6-day-old coldstressed individuals and those growing at 348C that were ABA-supplemented (Kruskal -Wallis test, N 348C,Control ¼ 6, figure 5c). In 8-day-old individuals that were either growing at 348C or submitted to cold stress, ABA supplementation induced a decrease of HSP70 transcript levels, suggesting that ABA-supplemented individuals might be sensing a less severe stress (two-way ANOVA, N 348C,Control ¼ 6, N 348C,ABA ¼ 6, N 258C,Control ¼ 6, N 258C,ABA ¼ 6, temperature: Lanthionine synthetase C-like protein (LANCL2) has been established as a G protein-coupled peripheral membrane protein and a key component of the ABA signal transduction pathway in mammalian cells [32,33]. Sequence alignment of human and A. mellifera LANCL2 shows 41.3% amino acid identity and 71% similarity (electronic supplementary material, figure S3).…”

Section: Resultsmentioning

confidence: 99%

Abscisic acid enhances cold tolerance in honeybee larvae

et al. 2017

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The natural composition of nutrients present in food is a key factor determining the immune function and stress responses in the honeybee (Apis mellifera). We previously demonstrated that a supplement of abscisic acid (ABA), a natural component of nectar, pollen, and honey, increases honeybee colony survival overwinter. Here we further explored the role of ABA in in vitro-reared larvae exposed to low temperatures. Four-day-old larvae (L4) exposed to 258C for 3 days showed lower survival rates and delayed development compared to individuals growing at a standard temperature (348C). Cold-stressed larvae maintained higher levels of ABA for longer than do larvae reared at 348C, suggesting a biological significance for ABA. Larvae fed with an ABA-supplemented diet completely prevent the low survival rate due to cold stress and accelerate adult emergence. ABA modulates the expression of genes involved in metabolic adjustments and stress responses: Hexamerin 70b, Insulin Receptor Substrate, Vitellogenin, and Heat Shock Proteins 70. AmLANCL2, the honeybee ABA receptor, is also regulated by cold stress and ABA. These results support a role for ABA increasing the tolerance of honeybee larvae to low temperatures through priming effects.

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Section: Resultsmentioning

confidence: 99%

Abscisic acid enhances cold tolerance in honeybee larvae

et al. 2017

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“…The WT-and K369A-NAMPT-p3XFLAG-CMV-9 plasmids were purified with the Endofree Plasmid Maxi Kit (Qiagen, Germantown, MD, USA) and sequenced by TibMolbiol (Genoa, Italy). HEK 293 cells (2 3 10 7 cells) were transfected, as in Fresia et al (29), to express different FLAG-tagged NAMPT forms (K53R, K79R, and K369A and the respective WT forms). At 48 h after transfection, the HEK 293 cells were lysed in immunoprecipitation buffer [PBS (pH 7.4), 0.5% NP-40, 1 mM EDTA, 1 mM NaF, 10 mM trichostatin A, 10 mM NAM, 0.5 mM DTT, and protease inhibitor cocktail), and cell extracts were incubated in the presence of monoclonal anti-FLAG-M2-conjugated Dynabeads Protein G (MilliporeSigma) for 2-3 h at 4°C.…”

Section: Immunopurification Of Wt and Mutant Namptmentioning

confidence: 99%

SIRT6 deacetylase activity regulates NAMPT activity and NAD(P)(H) pools in cancer cells

et al. 2018

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Nicotinamide phosphoribosyltransferase (NAMPT) is the rate‐limiting enzyme in the NAD+ salvage pathway from nicotinamide. By controlling the biosynthesis of NAD+, NAMPT regulates the activity of NAD+‐converting enzymes, such as CD38, poly‐ADP‐ribose polymerases, and sirtuins (SIRTs). SIRT6 is involved in the regulation of a wide number of metabolic processes. In this study, we investigated the ability of SIRT6 to regulate intracellular NAMPT activity and NAD(P)(H) levels. BxPC‐3 cells and MCF‐7 cells were engineered to overexpress a catalytically active or a catalytically inactive SIRT6 form or were engineered to silence endogenous SIRT6 expression. In SIRT6‐overexpressing cells, NAD(H) levels were up‐regulated, as a consequence of NAMPT activation. By immunopurification and incubation with recombinant SIRT6, NAMPT was found to be a direct substrate of SIRT6 deacetylation, with a mechanism that upregulates NAMPT enzymatic activity. Extracellular NAMPT release was enhanced in SIRT6‐silenced cells. Also glucose‐6‐phosphate dehydrogenase activity and NADPH levels were increased in SIRT6‐overexpressing cells. Accordingly, increased SIRT6 levels reduced cancer cell susceptibility to H2O2‐induced oxidative stress and to doxorubicin. Our data demonstrate that SIRT6 affects intracellular NAMPT activity, boosts NAD(P)(H) levels, and protects against oxidative stress. The use of SIRT6 inhibitors, together with agents inducing oxidative stress, may represent a promising treatment strategy in cancer.—Sociali, G., Grozio, A., Caffa, I., Schuster, S., Becherini, P., Damonte, P., Sturla, L., Fresia, C., Passalacqua, M., Mazzola, F., Raffaelli, N., Garten, A., Kiess, W., Cea, M., Nencioni, A., Bruzzone, S. SIRT6 deacetylase activity regulates NAMPT activity and NAD(P)(H) pools in cancer cells. FASEB J. 33, 3704–3717 (2019). http://www.fasebj.org

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“…ABA, via LANCL2 binding, stimulates insulin release and insulin‐independent glucose uptake in myocytes and adipocytes and induces brown fat activity . The downstream signalling pathway activated by ABA binding to LANCL2 involves the activation of a G protein, cyclic AMP production, with subsequent PKA‐mediated phosphorylation of CD38, and synthesis of the Ca 2+ ‐mobilizer cyclic ADP‐ribose . In addition, ABA binding induces the nuclear translocation of LANCL2, as also occurs after chemical or mutational demyristoylation .…”

Section: Discussionmentioning

confidence: 99%

“…The downstream signalling pathway activated by ABA binding to LANCL2 involves the activation of a G protein, cyclic AMP production, with subsequent PKA‐mediated phosphorylation of CD38, and synthesis of the Ca 2+ ‐mobilizer cyclic ADP‐ribose . In addition, ABA binding induces the nuclear translocation of LANCL2, as also occurs after chemical or mutational demyristoylation . Indeed, transcriptional effects of ABA have been reported in hemopoietic progenitor cells and in preadipocytes .…”

Section: Discussionmentioning

confidence: 99%

“…Autofluorescence was quenched with 5% glycerol in PBS (5 minutes) before blocking with 1x Roti‐Block in H 2 O with Fc‐Block (1:100), for 2 hours at RT in a humidified chamber. After three washings, the monoclonal anti‐LANCL2 was applied onto slices at a 1:150 dilution in 1x Roti‐Block and incubated in a humidified chamber overnight. After three washings, slices were incubated for 1 hour with a donkey‐anti‐mouse Cy3 antibody (715‐166‐151, Jackson ImmunoResearch), diluted 1:500 in 1x Roti‐Block.…”

Section: Methodsmentioning

confidence: 99%

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Abscisic acid stimulates the release of insulin and of GLP‐1 in the rat perfused pancreas and intestine

Booz

Kuhre

Saltiel

et al. 2018

Diabetes Metabolism Res

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Aims Previous results indicate that nanomolar concentrations of abscisic acid (ABA) stimulate insulin release from β‐pancreatic cells in vitro and that oral ABA at 50 mg/kg increases plasma GLP‐1 in the fasted rat. The aim of this study was to test the effect of ABA on the perfused rat pancreas and intestine, to verify the insulin‐ and incretin‐releasing actions of ABA in controlled physiological models. Materials and methods Rat pancreas and small intestine were perfused with solutions containing ABA at high‐micromolar concentrations, or control secretagogues. Insulin and GLP‐1 concentrations in the venous effluent were analysed by radioimmunoassay, and ABA levels were determined by ELISA. Results High micromolar concentrations of ABA induced GLP‐1 secretion from the proximal half of the small intestine and insulin secretion from pancreas. GLP‐1 stimulated ABA secretion from pancreas in a biphasic manner. Notably, a positive correlation was found between the ABA area under the curve (AUC) and the insulin AUC upon GLP‐1 administration. Conclusion Our results indicate the existence of a cross talk between GLP‐1 and ABA, whereby ABA stimulates GLP‐1 secretion, and vice versa. Release of ABA could be considered as a new promising molecule in the strategy of type 2 diabetes treatment and as a new endogenous hormone in the regulation of glycaemia.

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G-protein coupling and nuclear translocation of the human abscisic acid receptor LANCL2

Cited by 39 publications

References 56 publications

Abscisic acid enhances cold tolerance in honeybee larvae

Abscisic acid enhances cold tolerance in honeybee larvae

SIRT6 deacetylase activity regulates NAMPT activity and NAD(P)(H) pools in cancer cells

Abscisic acid stimulates the release of insulin and of GLP‐1 in the rat perfused pancreas and intestine

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