2016
DOI: 10.1002/smll.201601540
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G Protein-Coupled Receptors Incorporated into Rehydrated Diblock Copolymer Vesicles Retain Functionality

Abstract: We successfully incorporated the human serotonin receptor, G protein coupled receptor (GPCR), 5-HT1AR, in micron scale polymeric giant unilamellar protein-vesicles (pGUPs). By utilizing an agarose rehydration technique for protein incorporation, the GPCR is inserted in biased orientation with the C-terminus cytosolic and N-terminus extracellular as found in the cell plasma membrane. The GPCR is fully functional within the polymeric bilayer exhibiting responses to various ligands. The entire population of incor… Show more

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Cited by 7 publications
(3 citation statements)
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“…Giant polymersomes based on PB-b-PEO was prepared via agaroseassisted hydration method. [13,23] Briefly, 2% w/v agarose was dissolved in deionized water at 90 °C and spread onto a 25 mm-diameter #1 coverslip to form agarose gel. The polymer solution in chloroform (2 mg mL −1 ) with 0.5 mol% of Liss Rhod DMPE was spread on the agarose gel-coated coverslip to form a thin polymer film.…”
Section: Giant Polymersome Preparation Via Agarose-assisted Hydration Methodmentioning
confidence: 99%
“…Giant polymersomes based on PB-b-PEO was prepared via agaroseassisted hydration method. [13,23] Briefly, 2% w/v agarose was dissolved in deionized water at 90 °C and spread onto a 25 mm-diameter #1 coverslip to form agarose gel. The polymer solution in chloroform (2 mg mL −1 ) with 0.5 mol% of Liss Rhod DMPE was spread on the agarose gel-coated coverslip to form a thin polymer film.…”
Section: Giant Polymersome Preparation Via Agarose-assisted Hydration Methodmentioning
confidence: 99%
“…Furthermore, while conventional thinking says that block copolymers for IMP reconstitution should be in a fluid state, above their glass transition temperature, proteorhodopsin (PR) has been functionally reconstituted into highly stable glassy block copolymer membranes with PS hydrophobic blocks [ 43 ]. Proteopolymersomes have also shown stability under lyophilisation: following rehydration, PBd–PEO vesicles are restored without a significant loss of IMP function, in this case a G-protein-coupled receptor [ 50 ].…”
Section: Polymersomesmentioning
confidence: 99%
“…42 (2) Adding pore-forming proteins to induce the formation of channels in the polymeric membranes which enhances the polymersome permeability and allows for selective molecule exchange. 43 (3) Utilizing stimuli-responsive constituents to endow the polymersomes with triggered-induced (such as pH, CO 2 , light, temperature, enzyme, etc. [44][45][46][47][48] ) permeability control.…”
Section: Introductionmentioning
confidence: 99%