G-CSF Administration Accelerates Cutaneous Wound Healing Accompanied With Increased Pro-Hyp Production In db/db Mice

Jimi, Shiro

doi:10.15226/2378-1726/4/2/00155

Cited by 16 publications

(21 citation statements)

References 34 publications

(45 reference statements)

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“…Pro-Hyp and Hyp-Gly are the main hydroxyprolyl peptides found in human blood after ingestion of collagen hydrolysate [10][11][12][13]. Pro-Hyp is also generated by the degradation of endogenous collagen in tissue undergoing inflammation [14] and at wound healing sites in the skin [15].…”

Section: Introductionmentioning

confidence: 99%

Food-Derived Collagen Peptides, Prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) Enhance Growth of Primary Cultured Mouse Skin Fibroblast Using Fetal Bovine Serum Free from Hydroxyprolyl Peptide

Asai

Oikawa

Yoshikawa

et al. 2019

IJMS

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Prolyl-hydroxyproline (Pro-Hyp) and hydroxyprolyl-glycine (Hyp-Gly) appear in human blood after ingestion of collagen hydrolysate and trigger growth of fibroblasts attached on collagen gel, which has been associated with beneficial effects upon ingestion of collagen hydrolysate, such as improvement of skin and joint conditions. In the present study, inconsistent results were obtained by using different lots of fetal bovine serum (FBS). Fibroblasts proliferated in collagen gel without adding Pro-Hyp and Hyp-Gly and did not respond to addition of Pro-Hyp and Hyp-Gly, which raises doubts about conclusions from prior research. Unexpectedly high levels of hydroxyprolyl peptides, including Pro-Hyp, however, were present in the FBS (approximately 100 µM), and also in other commercially available forms of FBS (70-80 µM). After removal of low molecular weight (LMW, < 6000 Da) compounds from the FBS by size exclusion chromatography, Pro-Hyp and Hyp-Gly again triggered growth of fibroblasts attached on collagen and increased the number of fibroblasts migrated from mouse skin. These results indicate the presence of bioactive hydroxyprolyl peptides in commercially available FBS, which can mask effects of Pro-Hyp and Hyp-Gly supplementation; our work confirms that Pro-Hyp and Hyp-Gly do play crucial roles in proliferation of fibroblasts.

show abstract

Section: Introductionmentioning

confidence: 99%

Food-Derived Collagen Peptides, Prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) Enhance Growth of Primary Cultured Mouse Skin Fibroblast Using Fetal Bovine Serum Free from Hydroxyprolyl Peptide

Asai

Oikawa

Yoshikawa

et al. 2019

IJMS

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“…These endogenous peptides, if present, might be involved in wound healing and tissue reconstruction by enhancing the growth of fibroblasts and producing extracellular matrix compounds. Jimi et al (2017) demonstrated that Pro-Hyp is generated in the granulation tissue at a wound healing site on C57 BL/6 mice skin, while it does not increase in the normal skin of same mice (Fig. 1).…”

Section: Generation Of Pro-hyp By Degradation Of Endogenous Collagenmentioning

confidence: 88%

“…Jimi et al . () demonstrated that Pro‐Hyp is generated in the granulation tissue at a wound healing site on C57 BL/6 mice skin, while it does not increase in the normal skin of same mice (Fig. ).…”

Section: Generation Of Pro‐hyp By Degradation Of Endogenous Collagenmentioning

confidence: 97%

“…The two different animal studies demonstrate that Pro‐Hyp is generated in the granulation tissue (Jimi et al ., ) and ear with dermatitis (Kusubata et al ., ), where tissue reconstruction occurs. Fibroblast plays a significant role in wound healing and tissue reconstruction by secreting proteases and synthesising extracellular matrix compounds such as collagen and proteoglycans (des Jardins‐Park et al ., ).…”

Section: Conclusion and Further Prospectivementioning

confidence: 99%

“…Data are mean AE standard error (n = 3). This figure was adapted with permission (Jimi et al, 2017). subsequent degradation of endogenous collagen.…”

Section: Generation Of Pro-hyp By Degradation Of Endogenous Collagenmentioning

confidence: 99%

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Generation of bioactive prolyl‐hydroxyproline (Pro‐Hyp) by oral administration of collagen hydrolysate and degradation of endogenous collagen

Satô

Jimi

Kusubata

2019

Int J of Food Sci Tech

Self Cite

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Summary Hydroxyproline‐containing di‐ and tripeptides increase in human blood plasma after ingestion of collagen hydrolysate in a dose‐dependent manner. Prolyl‐hydroxyproline (Pro‐Hyp) and hydroxyprolyl‐glycine (Pro‐Gly) are the main food‐derived collagen peptides present in human blood plasma. Pro‐Hyp increases the number of fibroblasts migrating from the mouse skin and enhances the growth of fibroblasts attached to the collagen gel. Pro‐Hyp is also generated from endogenous collagen in the granulation tissue in mice skin and ear with dermatitis, while it does not increase in the normal tissues of the same animal. However, Hyp‐Gly does not increase in all tissues. These facts indicate that Pro‐Hyp is generated by the degradation of endogenous collagen to activate cells involved in tissue reconstruction. Orally administered Pro‐Hyp is deposited in animal organs even after clearance of Pro‐Hyp from blood. Thus, the endogenous bioactive peptide can be provided by ingestion of collagen hydrolysate.

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Food for Skin Health: Collagen Peptides

Satô

2019

Encyclopedia of Food Chemistry

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G-CSF Administration Accelerates Cutaneous Wound Healing Accompanied With Increased Pro-Hyp Production In db/db Mice

Cited by 16 publications

References 34 publications

Food-Derived Collagen Peptides, Prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) Enhance Growth of Primary Cultured Mouse Skin Fibroblast Using Fetal Bovine Serum Free from Hydroxyprolyl Peptide

Food-Derived Collagen Peptides, Prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) Enhance Growth of Primary Cultured Mouse Skin Fibroblast Using Fetal Bovine Serum Free from Hydroxyprolyl Peptide

Generation of bioactive prolyl‐hydroxyproline (Pro‐Hyp) by oral administration of collagen hydrolysate and degradation of endogenous collagen

Food for Skin Health: Collagen Peptides

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