G

Isolation of Co 5'-deoxyadenosylcobinamide guanosine diphosphate, α-(2-methyladenyl)-Co 5'-deoxyadenosylcobamide, and Co methylcorrinoids from Clostridium thermoaceticum

“…This was suggested by Ronzio and Barker (1967), who reported that Co-2,3-isopropylidene 5 '-deoxyadenosylcobinamide phosphate was a better substrate than DA-cobinamide phosphate in reaction 1 above. Müller and Müller (1963) have shown that Co-butylcobinamide is converted into Co-butylcobalamin by cells of P. shermanii. The presence of seven different Co-methylcorrinoids in C. thermoaceticum is an indication that Co-methyl derivatives also may be intermediates in the formation of complete factors.…”

Enzymic Synthesis of Guanosine Diphosphate Cobinamide by Extracts of Propionic Acid Bacteria*

“…rugosa. In the extracts examined, DIA-cobinamide phosphate was generally a better substrate than DAcobinamide phosphate. Müller and Müller (1963) reported that Co-butylcobinamide and Co-5'-deoxyinosylcobinamide are partially converted by intact cells of P. shermanii to Co-butylcobalamin and Co-deoxyinosylcobalamin. Therefore, the enzymes involved in the conversion of cobinamide to complete corrinoid compounds require an alkyl group on the cobalt, but lack specificity for the type of alkyl group.…”

Corrinoid Compounds of Methanobacillus omelianskii. I. Fractionation of the Corrinoid Compounds and Identification of Factor III and Factor III Coenzyme*

“…This coenzyme was synthesized according to the general method of Muller and Muller (1963). To a solution of 4.5 mg Factor I11 in 0.8 ml of water about 1 mg of PtOz was added.…”