FzlA, an essential regulator of FtsZ filament curvature, controls constriction rate during <i>Caulobacter</i> division

Lariviere, Patrick J.; Szwedziak, Piotr; Mahone, Christopher R.; Löwe, Jan; Goley, Erin D.

doi:10.1111/mmi.13876

Cited by 29 publications

(60 citation statements)

References 61 publications

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“…In the hypothetical case of extremely rapid constriction, the cell length would be set almost entirely by the growth during the pure elongation stage, leading to short cells with blunt poles. By modulating constriction onset and rate together ( Figure 4 E), cells may arrive at a variety of pole shapes, an emerging control mechanism for bacterial cell shape ( Lariviere et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus

et al. 2018

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SummaryRod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral walls and then via a combination of zonal elongation and constriction at the division site to form the poles of daughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size control and homeostasis has rarely been considered. To reveal the roles of cell elongation and constriction in bacterial size regulation during cell division, we captured the shape dynamics of Caulobacter crescentus with time-lapse structured illumination microscopy and used molecular markers as cell-cycle landmarks. We perturbed the constriction rate using a hyperconstriction mutant or fosfomycin ([(2R,3S)-3-methyloxiran-2-yl]phosphonic acid) inhibition. We report that the constriction rate contributes to both size control and homeostasis, by determining elongation during constriction and by compensating for variation in pre-constriction elongation on a single-cell basis.

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Section: Discussionmentioning

confidence: 99%

Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus

et al. 2018

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“…FtsZ 1 and/or FtsZ AT were polymerized in HEK50 with 2.5 mM MgCl 2 and 2 mM GTP. After a 15 min incubation at room temperature, samples were applied to carbon-coated glow-discharged grids with 0.75% uranyl formate staining as previously described (Sundararajan et al, 2015;Lariviere et al, 2018). TEM samples were imaged using a Philips/ FEI BioTwin CM120 TEM equipped with an AMT XR80 8 megapixel CCD camera (AMT Imaging, USA).…”

Section: Negative Stain Transmission Electron Microscopymentioning

confidence: 99%

Agrobacterium tumefaciens divisome proteins regulate the transition from polar growth to cell division

Howell

Aliashkevich

Sundararajan

et al. 2019

Molecular Microbiology

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Summary The mechanisms that restrict peptidoglycan biosynthesis to the pole during elongation and re‐direct peptidoglycan biosynthesis to mid‐cell during cell division in polar‐growing Alphaproteobacteria are largely unknown. Here, we explore the role of early division proteins of Agrobacterium tumefaciens including three FtsZ homologs, FtsA and FtsW in the transition from polar growth to mid‐cell growth and ultimately cell division. Although two of the three FtsZ homologs localize to mid‐cell, exhibit GTPase activity and form co‐polymers, only one, FtsZAT, is required for cell division. We find that FtsZAT is required not only for constriction and cell separation, but also for initiation of peptidoglycan synthesis at mid‐cell and cessation of polar peptidoglycan biosynthesis. Depletion of FtsZAT in A. tumefaciens causes a striking phenotype: cells are extensively branched and accumulate growth active poles through tip splitting events. When cell division is blocked at a later stage by depletion of FtsA or FtsW, polar growth is terminated and ectopic growth poles emerge from mid‐cell. Overall, this work suggests that A. tumefaciens FtsZ makes distinct contributions to the regulation of polar growth and cell division.

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“…Additionally, this also implicates species-specific regulatory factors in vivo in modifying the assembly properties of FtsZ to generate a Z-ring with the appropriate dynamics and structure to effect division. The repertoire of Z-ring associated proteins that affect protofilament bundling and/or turnover is vast and varies across species (Gueiros-Filho and Losick, 2002;Mohammadi et al, 2009;Goley et al, 2010;Galli and Gerdes, 2011;Durand-Heredia et al, 2012;Woldemeskel et al, 2017;Lariviere et al, 2018). For example, while FzlA, an essential protein that binds and assembles FtsZ filaments into helical bundles in vitro, is conserved in alpha-proteobacteria including C. crescentus, it is absent from other bacteria including E. coli (Goley et al, 2010;Lariviere et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Species‐ and C‐terminal linker‐dependent variations in the dynamic behavior of FtsZ on membranes in vitro

Sundararajan

Vecchiarelli

Mizuuchi

et al. 2018

Molecular Microbiology

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Bacterial cell division requires the assembly of FtsZ protofilaments into a dynamic structure called the 'Z-ring'. The Z-ring recruits the division machinery and directs local cell wall remodeling for constriction. The organization and dynamics of protofilaments within the Z-ring coordinate local cell wall synthesis during cell constriction, but their regulation is largely unknown. The disordered C-terminal linker (CTL) region of Caulobacter crescentus FtsZ (CcFtsZ) regulates polymer structure and turnover in solution in vitro, and regulates Z-ring structure and activity of cell wall enzymes in vivo. To investigate the contributions of the CTL to the polymerization properties of FtsZ on its physiological platform, the cell membrane, we reconstituted CcFtsZ polymerization on supported lipid bilayers (SLB) and visualized polymer dynamics and structure using total internal reflection fluorescence microscopy. Unlike Escherichia coli FtsZ protofilaments that organized into large, bundled patterns, CcFtsZ protofilaments assembled into small, dynamic clusters on SLBs. Moreover, CcFtsZ lacking its CTL formed large networks of straight filament bundles that underwent slower turnover than the dynamic clusters of wildtype FtsZ. Our in vitro characterization provides novel insights into species- and CTL-dependent differences between FtsZ assembly properties that are relevant to Z-ring assembly and function on membranes in vivo.

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FzlA, an essential regulator of FtsZ filament curvature, controls constriction rate during Caulobacter division

Cited by 29 publications

References 61 publications

Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus

Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus

Agrobacterium tumefaciens divisome proteins regulate the transition from polar growth to cell division

Species‐ and C‐terminal linker‐dependent variations in the dynamic behavior of FtsZ on membranes in vitro

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