FXYD2c Plays a Potential Role in Modulating Na+/K+-ATPase Activity in HK-2 Cells Upon Hypertonic Challenge

Chang, Chun-Yu; Tang, Cheng-Hao; Hsin, Yi-Hong; Lai, Hsing-Tzu; Lee, Tsung‐Han

doi:10.1007/s00232-013-9615-y

Cited by 9 publications

(2 citation statements)

References 35 publications

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“…NKA is mostly located in the gill ionocytes, in the intestinal absorbing enterocytes and in the ionocytes found along the tubules of the kidney. NKA contains the α, β and γ subunits (Blanco & Mercer, ; Geering, ; Chang et al ., ). The catalytic α‐subunit contains the binding sites for ATP, Na + and K + , whilst the β‐subunit allows the folding and placement of the α‐subunit into the cell membrane and the modulation of the enzyme K + and Na + affinity (Blanco & Mercer, ).…”

Section: Introductionmentioning

confidence: 97%

Effects of different salinities on the osmoregulatory capacity of Mediterranean sticklebacks living in freshwater

et al. 2017

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Three‐spined sticklebacks (Gasterosteus aculeatus L.) of the Camargue region (Rhone delta, northern Mediterranean Sea) occupy coastal mesohaline lagoons and freshwater canals. In this study, the acclimation capacity to different salinities of a population living in freshwater canals was investigated. Freshwater (FW; 5 ‰) individuals were compared to fish acclimated for at least 2 weeks to brackish water (BW; 15 ‰) and seawater (SW; 30 ‰). Blood osmotic pressure and gill Na+/K+‐ATPase (NKA) gene expression of the α1 subunit and α1a and α1b isoforms were determined at these salinities. NKA protein expression in the gill ionocytes and the remodelling of these cells were also investigated through NKA immunolabelling and electron microscopy. Blood osmolalities of fish acclimated to FW, BW and SW were significantly different. Branchial NKA α1a and α1b expressions also differed, with a higher expression of NKA α1b in fish acclimated to SW. Gill ionocytes in fish acclimated to FW were located along the lamellae and at their base and appeared elongated, whereas these cells were restricted to gill filaments in fish acclimated to SW with a round shape. Finally, electron microscopy revealed three different types of apical structures for these ionocytes: a honeycomb‐like structure and a dome shape in FW, and deeply encrypted in SW. This study shows that ionocyte cell type and morphology, and gill NKA expression are salinity‐dependent. This branchial remodelling is directly linked to the physiological homeostatic status reached by the fish. It also highlights that sticklebacks of the Camargue region can rapidly acclimate to different salinities and can easily migrate between FW canals and mesohaline coastal lagoons.

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Section: Introductionmentioning

confidence: 97%

Effects of different salinities on the osmoregulatory capacity of Mediterranean sticklebacks living in freshwater

et al. 2017

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“…Na–K-ATPase has been shown to play a fundamental role in the formation and maintenance of epithelial TJs [ 17 ]. Potassium is an essential electrolyte that is involved in various cellular homeostatic functions, including regulation and maintenance of cell volume, enzyme activity and DNA/protein synthesis [ 18 , 19 ]. Decreased potassium concentration in monocyte macrophages is a factor in the activation of Nod-like receptor protein-3 inflammasomes.…”

Section: Introductionmentioning

confidence: 99%

Low potassium disrupt intestinal barrier and result in bacterial translocation

Huang

Fan

et al. 2022

J Transl Med

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Background Bacterial translocation was observed in critical illness and patients with chronic diseases such as liver cirrhosis and chronic kidney disease (CKD). Hypokalemia is a common complication in these diseases. Whether low potassium diet may increase intestinal permeability and result in bacterial translocation lack of evidence. The present study was aimed to investigate the potential effects of LK on intestinal permeability. Methods Grade 8-week-old male Bal B/C mice were randomly placed either on a normal potassium (NK) mouse chow or a low potassium (LK) diet for 28 days. Intestinal permeability and expression of tight junction proteins were compared between the two groups. Results Compared with the NK group, the mice in LK group had significantly lower serum potassium level, increased levels of plasmas endotoxin and plasma d-lactate. The bacterial translocation was higher and in occurred mainly in mesenteric lymph nodes (MLN), liver and spleen. The pathologic change of small intestine was obvious with thinner villus lamina propria, shorter crypt depth and thinner intestinal wall. Slight increases in the expression of proteins and mRNA levels of both claudin-1 and claudin-2 were observed in LK group. Conclusions Low potassium diet could increase intestinal permeability and thereby lead to bacterial translocation, which was suspected to result from impaired intestinal epithelial barrier and biological barrier.

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Molecular characterization and expression of Na+/K+-ATPase α1 isoforms in the European sea bass Dicentrarchus labrax osmoregulatory tissues following salinity transfer

Blondeau‐Bidet

Bossus

Maugars

et al. 2016

Fish Physiol Biochem

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The Na/K-ATPase (NKA) is considered as the main pump involved in active ion transport. In the European sea bass, Dicentrarchus labrax, we found two genes encoding for the alpha 1 subunit isoforms (NKA α1a and NKA α1b). NKA α1a and NKA α1b isoform amino acid (aa) sequences were compared through phylogeny and regarding key functional motifs between salmonids and other acanthomorph species. Analysis of aa sequences of both isoforms revealed a high degree of conservation across teleosts. The expression pattern of both nka α1a and nka α1b was measured in the gill, kidney and posterior intestine of fish in seawater (SW) and transferred to fresh water (FW) at different exposure times. Nka α1a was more expressed than nka α1b whatever the condition and the tissue analyzed. After long-term salinity acclimation (2.5 years) either in FW or SW, transcript levels of nka α1a were higher in the kidney followed by the posterior intestine and the gill. Compared to SW conditions, expression of nka α1a in FW was significantly increased or decreased, respectively, in gill and posterior intestine. In contrast, branchial nka α1b was significantly decreased in FW-acclimated fish. Short-term FW acclimation seems to rapidly increase nka α1a transcript levels in the kidney unlike in gill tissues where different gene expression levels are detected only after long-term acclimation.

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FXYD2c Plays a Potential Role in Modulating Na+/K+-ATPase Activity in HK-2 Cells Upon Hypertonic Challenge

Cited by 9 publications

References 35 publications

Effects of different salinities on the osmoregulatory capacity of Mediterranean sticklebacks living in freshwater

Effects of different salinities on the osmoregulatory capacity of Mediterranean sticklebacks living in freshwater

Low potassium disrupt intestinal barrier and result in bacterial translocation

Molecular characterization and expression of Na+/K+-ATPase α1 isoforms in the European sea bass Dicentrarchus labrax osmoregulatory tissues following salinity transfer

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