Abstract-Biological processes are complex, nonlinear uncertain systems and the state variables are not all available for measurement. For that, fuzzy modeling and state estimations are powerful methods for the control and diagnosis of these kind of systems. In this paper, we present some results obtained from the design of fuzzy controller and fuzzy sliding mode observer for an anaerobic digestion system. Index Terms-Anaerobic digester, fuzzy controller, linear matrix inequalities, robust state estimation, T-S fuzzy modeling.