Fuzzy Predictions Regarding In Vitro Behavior of Cells Line Under Cytostatic Treatment

Ciprofloxacin-Collagen-Based Materials with Potential Oral Surgical Applications

“…The collagen-based materials with identical mass were introduced in the culture mediums of Vascular Cell Basal Medium (ATCC PCS–100030) with HUVEC viable cells. After 6 h, 24 h, and respectively 48 h, the solid materials were pull out from de mediums, and the numbers of viable cells were quantified using the MTT method [ 38 , 42 , 43 , 44 , 45 ]. All determinations have been done in plates of 96 wells, in triplicate, each determination being performed for 3 times.…”

“…Taking into consideration that these materials are envisaged for head and neck surgery cytotoxicity tests were performed using human umbilical vein endothelial cells (HUVEC) knowing their properties to mimic human normal cells organism reactions to various drugs. The HUVEC cells line is often used in comparative studies aimed to evaluate in vitro the effect of different therapeutic schemes on both normal cells and tumor cells [ 38 , 39 ], in order to quantify their cytotoxicity on human healthy cells. Moreover, they are used in laboratories as model systems for studying the function and pathology of endothelial cells.…”