“Fuzzy hair” promotes cell sheet detachment from thermoresponsive brushes already above their volume phase transition temperature

Stöbener, Daniel David; Weinhart, Marie

doi:10.1016/j.bioadv.2022.213101

Cited by 2 publications

(1 citation statement)

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“…[24] Recently it was shown that cell selectivity can be improved by using a PNIPAAm-based copolymer with hydrophobic comonomers, which leads to a decrease in transition temperature. [25][26][27] One example that is relevant in the context of this report is the work of Nagase et al, [28] who demonstrated enabled by carefully tuned polymer hydrophobicity and temperature-modulated properties the separation of HUVECs from coculture normal human dermal fibroblasts (NHDF) via thermoresponsive poly(N-isopropylacrylamideco-butyl methacrylate) brushes. These authors reported yields of 48% HUVEC with an impurity of 17% NHDF.…”

Section: Introductionmentioning

confidence: 99%

Mild Quantitative One Step Removal of Macrophages from Cocultures with Human Umbilical Vein Endothelial Cells Using Thermoresponsive Poly(Di(Ethylene Glycol)Methyl Ether Methacrylate) Brushes

Hebel,

Schönherr

2023

Macromolecular Bioscience

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The authors report on a mild, label‐free, and fast method for the separation of human umbilical vein endothelial cells (HUVEC), which are relevant cells, whose use is not limited to studies of endothelial dysfunction, from cocultures with macrophages to afford HUVEC in ≈100% purity. Poly(di(ethylene glycol)methyl ether methacrylate) (PDEGMA) brushes with a dry thickness of (5 ± 1) nm afford the highly effective one‐step separation by selective HUVEC detachment, which is based on the brushes' thermoresponsive behavior. Below the thermal transition at 32 °C the brushes swells and desorbs attached proteins, resulting in markedly decreased cell adhesion. Specifically, HUVEC and macrophages, which are differentiated from THP‐1 monocytes, are seeded and attached to PDEGMA brushes at 37°C. After decreasing the temperature to 22°C, HUVEC shows a decrease in their cell area, while the macrophages are not markedly affected by the temperature change. After mild flushing with a cell culture medium, the HUVEC can be released from the surface and reseeded again with ≈100% purity on a new surface. With this selective cell separation and removal method, it is possible to separate and thereby purify HUVEC from macrophages without the use of any releasing reagent or expensive labels, such as antibodies.

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