Future Paths in Cryogenic Single-Molecule Fluorescence Spectroscopy

Abstract

In the last three decades, cryogenic single-molecule fluorescence spectroscopy has provided average-free understanding of the photophysics and of fundamental interactions at molecular scales. Furthermore, they propose original pathways and applications in the treatment and storage of quantum information. The ultranarrow lifetime-limited zero-phonon line acts as an excellent sensor to local perturbations caused either by intrinsic dynamical degrees of freedom, or by external perturbations, such as those caused by electric fields, elastic and acoustic deformations, or light-induced dynamics. Single aromatic hydrocarbon molecules, being sensitive to nanoscale probing at nanometer scales, are potential miniaturized platforms for integrated quantum photonics. In this Perspective, we look back at some of the past advances in cryogenic optical microscopy and propose some perspectives for future development.

Chemo-Enzymatic Generation of Highly Fluorescent Nucleoside Analogs Using Purine-Nucleoside Phosphorylase

“…Fluorescence spectroscopy is one of the most sensitive methods of biomolecules detection and measurements of their interactions and activities, at both the ensemble and single-molecule levels [ 1 , 2 , 3 ]. For this reason, various fluorescence techniques are frequently used in biological, biochemical, and biophysical research.…”