Future Directions in Mesothelial Transplantation Research

Mutsaers, Steve; Paolo, N. Di

doi:10.1177/039139880703000614

Cited by 12 publications

(12 citation statements)

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“…These angiogenic and antiinflammatory effects together with production of many proteins useful in repopulating infarcted myocardium might limit LV remodeling. 15,16 It is reasonable to assume that we could obtain the maximum synergistic angiogenic effect in the infarcted scar when MC are transfected ex vivo by angiogenic genes such as stromal cell-derived factor-1a or vascular endothelial growth factor. The synergistic transient overexpression of these proteins in the scar should increase their concentration in the heart.…”

Section: Discussionmentioning

confidence: 99%

Mesothelial Cells vs. Skeletal Myoblasts for Myocardial Infarction

Bourahla

Shafy

Meilhac³

et al. 2010

Asian Cardiovasc Thorac Ann

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Cell transplantation for the regeneration of ischemic myocardium is limited by poor graft viability and low cell retention. Omental flaps in association with growth factors and cell sheets have recently been used to increase the vascularization of ischemic hearts. This experimental study was undertaken to evaluate the hemodynamic evolution and histological modifications of infarcted myocardium treated with mesothelial cells, and to compare the results with those of hearts treated with skeletal myoblasts. Myocardial infarction was created by surgical ligature of 2 coronary branches in 34 sheep; 6 died immediately due to ventricular fibrillation. Mesothelial cells were isolated from greater omentum, and myoblasts from skeletal muscle. After expanding the cells for 3 weeks, infarcted areas were treated with culture medium (control group), mesothelial cells, or myoblasts. After 3 months, echocardiographic studies showed significant limitation of ventricular dilatation and improved ejection fractions in both cell-treated groups compared to the controls. In the mesothelial cell group, histological studies showed significantly more angiogenesis and arteriogenesis than in the control and skeletal myoblast groups. Mesothelial cells might be useful for biological revascularization in patients with ischemic heart disease.

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Section: Discussionmentioning

confidence: 99%

Mesothelial Cells vs. Skeletal Myoblasts for Myocardial Infarction

Bourahla

Shafy

Meilhac³

et al. 2010

Asian Cardiovasc Thorac Ann

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“…A similar procedure was performed in patients with acute peritonitis receiving PD who were given mesothelial cells propagated in culture. In the peritoneal biopsy performed 3 and 6 days after the transplantation, a damaged peritoneum surface inlaid with transplanted cells was reported (33). Positively charged mesothelial cells are attracted by the negatively charged peritoneal surface at the mesothelial defect.…”

Section: Mesothelial Cell Transplantationmentioning

confidence: 99%

“…Within 3 days, a layer of transplanted mesothelial cells was reported on the damaged peritoneal surface. These cells were identified through the presence of large vacuoles and microvilli on the surface of the whole cell . A similar procedure was performed in patients with acute peritonitis receiving PD who were given mesothelial cells propagated in culture.…”

Section: Mesothelial Cell Transplantationmentioning

confidence: 99%

Therapeutic Applications of Mesothelial Cell Sheets

et al. 2014

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Mesothelial cells are an integral part of the peritoneum and play an important role in maintaining its structural and functional properties. In recent years a number of studies on mesothelial cells have been performed to evaluate the localization, secretional properties and the ability of regeneration and transdifferentiation of these cells. They are also involved in the repair of the peritoneum damage following surgery or peritonitis. Mesothelial cells produce several cytokines, growth factors and extracellular matrix components, possessing anti-inflammatory and immunomodulatory properties. Based on previous research, cell sheet engineering has made it possible to transplant cells that retain the cells' function, and stacking different cells in layers has also become possible. Arranging blood vessels between the cell layers is a problem when stacking cells in layers. Whether adequate blood flow can be obtained for the cell layers to survive long-term is the difference between success and failure. Mesothelial cell transplantation for peritoneal regeneration needs to be performed under conditions in which the surface area of the visceral peritoneum is large and the mesothelial cell damage area is small. In this article we explain cell sheet engineering as one of the technologies for transplanting cells with a variety of intercellular adhesion and cell membrane molecules maintained intact, and its application to peritoneal regeneration.

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“…These observations may have some practical implications, e.g., during peritoneal mesothelial cell transplantation. The procedure has been proposed to enhance mesothelial regeneration after surgery-or peritoneal dialysis-induced trauma and thus prevent peritoneal adhesion formation (32). In addition, transplantation of mesothelial cells have been used to improve vascularization of the ischemic myocardium (14) and to reconstruct the bladder (48).…”

Section: Ink4amentioning

confidence: 99%

“…In addition, transplantation of mesothelial cells have been used to improve vascularization of the ischemic myocardium (14) and to reconstruct the bladder (48). There is an ongoing debate on how to optimally isolate and propagate cells to be transplanted (32). In this respect, the fact that peritoneal mesothelial cell strains exhibit different senescence rates may be of importance if such procedures are to be effective.…”

Section: Ink4amentioning

confidence: 99%

Vulnerability to oxidative stress and different patterns of senescence in human peritoneal mesothelial cell strains

Książek

Mikuła-Pietrasik²,

Olijslagers³

et al. 2009

American Journal of Physiology-Regulatory, Integrative and Comp

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Ksiazek K, Mikula-Pietrasik J, Olijslagers S, Jörres A, von Zglinicki T, Witowski J. Vulnerability to oxidative stress and different patterns of senescence in human peritoneal mesothelial cell strains. Am J Physiol Regul Integr Comp Physiol 296: R374 -R382, 2009. First published November 26, 2008 doi:10.1152/ajpregu.90451.2008.-Both the ascites fluid-derived mesothelial cell line LP-9 and primary cultures of human omentum-derived mesothelial cells (HOMCs) are commonly used in experimental studies. However, they seem to have a different replicative potential in vitro. In the present study, we have attempted to determine the causes of this discrepancy. HOMCs were found to divide fewer times and enter senescence earlier than LP-9 cells. This effect was coupled with earlier increases in the expression of senescence-associated-␤-galactosidase and cell cycle inhibitors p16INK4a and p21 WAF1 . Moreover, almost 3 times as many earlypassage HOMCs as LP-9 cells bore senescence-associated DNA damage foci. In sharp contrast to LP-9 cells, the foci present in HOMCs localized predominantly outside the telomeres, and the HOMC telomere length did not significantly shorten during senescence. Compared with LP-9 cells, HOMCs were found to enter senescence with significantly lower levels of lipofuscin and damaged DNA, and markedly decreased glutathione contents. In addition, early-passage HOMCs generated significantly more reactive oxygen species either spontaneously or in response to exogenous oxidants. These results indicate that compared with LP-9 cells, HOMCs undergo stress-induced telomere-independent premature senescence, which may result from increased vulnerability to oxidative DNA injury. DNA damage; peritoneal mesothelial cells CELLULAR SENESCENCE IS A STATE in which somatic cells stop dividing and acquire properties that distinguish them clearly from both proliferating and quiescent cells. The senescence phenotype includes irreversible growth arrest in G 1 phase of the cell cycle, hypertrophic morphology, increased expression of senescence-associated ␤-galactosidase (SA-␤-Gal), accumulation of senescence-associated DNA damage foci and increased expression of cell-cycle inhibitors p16INK4a and p21 WAF1

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Future Directions in Mesothelial Transplantation Research

Cited by 12 publications

References 0 publications

Mesothelial Cells vs. Skeletal Myoblasts for Myocardial Infarction

Mesothelial Cells vs. Skeletal Myoblasts for Myocardial Infarction

Therapeutic Applications of Mesothelial Cell Sheets

Vulnerability to oxidative stress and different patterns of senescence in human peritoneal mesothelial cell strains

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