Future developments in biosensors for field-ready Zika virus diagnostics

Nicolini, Ariana M.; McCracken, Katherine E.; Yoon, Jeong Yeol

doi:10.1186/s13036-016-0046-z

Cited by 46 publications

(60 citation statements)

References 60 publications

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“…Emerging infectious diseases pose serious public health concern, particularly in rural and resource‐limited settings where prevention is hindered by the extensive time, materials, instrumentation, or infrastructure required by conventional diagnostics . Reflecting on the 2015–2016 Zika virus (ZIKV) outbreak, researchers must explore inexpensive, rapid, portable, and versatile pathogen diagnostics to focus limited resources in field and clinical settings …”

Section: Figurementioning

confidence: 99%

“…This included quantification of net interfacial tension, contact angle, viscosity, and fiber swelling/pore deformation changes (Figure ). These data were used to develop a L –W/C–B capillary flow model, which was validated by a capillary flow dynamics‐based assay for E. coli K12, a well‐characterized bacterial target used in prior scattering‐based immunoagglutination assays, and ZIKV, a virus of recent concern …”

Section: Figurementioning

confidence: 99%

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A Capillary Flow Dynamics‐Based Sensing Modality for Direct Environmental Pathogen Monitoring

Klug

Reynolds

Yoon

2018

Chemistry A European J

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Toward ultra-simple and field-ready biosensors, we demonstrate a novel assay transducer mechanism based on interfacial property changes and capillary flow dynamics in antibody-conjugated submicron particle suspensions. Differential capillary flow is tunable, allowing pathogen quantification as a function of flow rate through a paper-based microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target-particle aggregation. This mechanism is demonstrated for assays of Escherichia coli K12 in water samples and Zika virus (ZIKV) in blood serum. These assays achieved very low limits of detection compared with other demonstrated methods (1 log CFU/mL E. coli and 20 pg/mL ZIKV whole virus) with an operating time of 30 s, showing promise for environmental and health monitoring.

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Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

A Capillary Flow Dynamics‐Based Sensing Modality for Direct Environmental Pathogen Monitoring

Klug

Reynolds

Yoon

2018

Chemistry A European J

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“…Compounds chloromonilicin (3), a-costic acid (4), fusaric acid (8), inuloxin A (13) and 3-nitroprpionic acid (16) showed >90% mortality at the 1.0 µg/µL; however, the activity declined quickly as the compound was diluted. In adult bioassays, compounds (20), pyripyropene A (18), pyripyropene E (19), haemanthidine (11), sphaeropsidin C (21), haemanthamine (10) and cytochalasin A (6) exhibited moderate activity and weak activity was displayed by buphanamine (1), chloromonilicin (3), cytochalasin B (7), inuloxin C (14) and 6-hydroxymellein (12).…”

Section: Resultsmentioning

confidence: 99%

“…In adult bioassays, compounds were tested at the pre-screening dose of 5 µg/mosquito and only four compounds, α-costic acid (4), fusaric acid (8), gliotoxin (9) and 3-nitropropionic acid (16), demonstrated mortality between 83-97% ( Table 2). Based on this initial screening activity data, LD 50 bioassays were conducted and compound 8 was the most effective compounds with an LD 50 value of 0.8349 ± 0.0118 µg/mosquito and followed by compounds 16 (LD 50 value= 1.6641 ± 0.0494 µg/mosquito), 4 (LD 50 value= 2.547 ± 0.0835 µg/mosquito), 9 (LD 50 value= 2.79 ± 0.1197 µg/mosquito) ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…aegypti is also one of the vectors of Zika virus [7]. The clinical presentation of Zika fever is nonspecific and can be misdiagnosed as the first infections were associated with flu-like symptoms [8]. In addition, severe neurological complications were reported in the French Polynesian outbreak in 2013 and 2014, and later a dramatic increase in congenital malformations (microcephaly) were reported from Zika emergence in Brazil [7].…”

Section: Introductionmentioning

confidence: 99%

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A survey of bacterial, fungal and plant metabolites against Aedes aegypti (Diptera: Culicidae), the vector of yellow and dengue fevers and Zika virus

et al. 2017

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Aedes aegypti L. is the major vector of the arboviruses responsible for dengue fever, one of the most devastating human diseases. Some bacterial, fungal and plant metabolites belonging to different chemical subgroups, including Amaryllidaceae alkaloids, anthracenes, azoxymethoxytetrahydropyrans, cytochalasans, 2,5-diketopiperazines, isochromanones, naphthoquinones, organic small acids and their methyl esters, sterols and terpenes including sesquiterpenes and diterpenes, were tested for their larvicidal and adulticidal activity against Ae. aegypti. Out of 23 compounds tested, gliotoxin exhibited mosquitocidal activity in both bioassays with an LC 50 value of 0.0257 ± 0.001 µg/ µL against 1 st instar Ae. aegypti and LD 50 value of 2.79 ± 0.1197 µg/mosquito against adult female Ae. aegypti. 2-Methoxy-1,4-naphthoquinone and cytochalasin A showed LC 50 values of 0.0851 ± 0.0012 µg/µL and 0.0854 ± 0.0019 µg/µL, respectively, against Ae. aegypti larvae. In adult bioassays, fusaric acid (LD 50 = 0.8349 ± 0.0118 µg/ mosquito), 3-nitropropionic acid (LD 50 = 1.6641 ± 0.0494 µg/mosquito) and α-costic acid (LD 50 = 2.547 ± 0.0835 µg/ mosquito) exhibited adulticidal activity. Results from the current study confirm that compounds belonging to cytochalsin, diketopiperazine, naphthoquinone and low molecular weight organic acid groups are active and may stimulate further SAR investigations.

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Development of disposable electrode for the detection of mosquito‐borne viruses

Nasrin

Khoris

Chowdhury

et al. 2023

Biotechnology Journal

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Development of disposable, rapid, and convenient biosensor with high sensitivity and reliability is the most desired method of viral disease prevention. To achieve this goal, in this work, a practical impedimetric biosensor has been implemented into a disposable electrode on a screen‐printed carbon electrode (SPCE) for the detection of two mosquito‐borne viruses. The biosensor fabrication has step‐wisely carried out on the disposable electrode surface at room temperature: starting from conductive film formation, physical binding of the gold nanoparticles (AuNPs)‐polyaniline (PAni) into the conductive film, and biofunctionalization. To get the maximum efficiency of the antibody, biotinylated antibody has been conjugated on the surface of AuNP‐PAni/PAni‐SPCE via the streptavidin‐biotin conjugation method which is a critical factor for the high sensitivity. Using the antibody–antigen interaction, this disposable electrode has designed to detect mosquito‐borne infectious viruses, Chikungunya virus (CHIKV), and Zika virus (ZIKV) separately in a wide linear range of 100 fg mL−1 to 1 ng mL−1 with a low detection limit of 1.33 and 12.31 fg mL−1, respectively.

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Future developments in biosensors for field-ready Zika virus diagnostics

Cited by 46 publications

References 60 publications

A Capillary Flow Dynamics‐Based Sensing Modality for Direct Environmental Pathogen Monitoring

A Capillary Flow Dynamics‐Based Sensing Modality for Direct Environmental Pathogen Monitoring

A survey of bacterial, fungal and plant metabolites against Aedes aegypti (Diptera: Culicidae), the vector of yellow and dengue fevers and Zika virus

Development of disposable electrode for the detection of mosquito‐borne viruses

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