Fusogenic liposome-coated nanoparticles for rapid internalization into donor corneal endothelial tissue to enable prophylaxis before transplantation

M. Y., Thanuja; Tellakula, Suraksha S.; Suryavanshi, Samarth V.; G. S., Keerthana; Vasudev S., Chandan; Ranganath, Sudhir H.

doi:10.1039/d3na00535f

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Nanoscale Adv.

2023

DOI: 10.1039/d3na00535f

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Fusogenic liposome-coated nanoparticles for rapid internalization into donor corneal endothelial tissue to enable prophylaxis before transplantation

Thanuja M. Y.,

Suraksha S. Tellakula,

Samarth V. Suryavanshi

et al.

Abstract: Fusogenic liposome-coated nanoparticles rapidly internalize into ex vivo donor corneal endothelium within 3 hours of incubation at physiological temperature possibly via the non-endocytic, membrane fusion mechanism. Scale bar is 10 μm

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2024

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Cited by 2 publications

References 54 publications

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Suppressing Pro-Apoptotic Proteins by siRNA in Corneal Endothelial Cells Protects against Cell Death

Staehlke,

Mahajan,

Thieme

et al. 2024

Biomedicines

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Corneal endothelial cells (CE) are critical for the cornea’s transparency. For severe corneal damage, corneal tissue transplantation is the most promising option for restoring vision. However, CE apoptotic cell death occurs during the storage of donor corneas for transplantation. This study used small interfering (si)RNA-mediated silencing of pro-apoptotic proteins as a novel strategy to protect CE against apoptosis. Therefore, the pro-apoptotic proteins Bax and Bak were silenced in the human corneal endothelial cell line (HCEC-12) by transfection with Accell™siRNA without any adverse effects on cell viability. When apoptosis was induced, e.g., etoposide, the caspase-3 activity and Annexin V-FITC/PI assay indicated a significantly reduced apoptosis rate in Bax+Bak-siRNA transfected HCECs compared to control (w/o siRNA). TUNEL assay in HCECs exposed also significantly lower cell death in Bax+Bak-siRNA (7.5%) compared to control (w/o siRNA: 32.8%). In ex vivo donor corneas, a significant reduction of TUNEL-positive CEs in Bax+Bak-siRNA corneas (8.1%) was detectable compared to control-treated corneas (w/o siRNA: 27.9%). In this study, we demonstrated that suppressing pro-apoptotic siRNA leads to inhibiting CE apoptosis. Gene therapy with siRNA may open a new translational approach for corneal tissue treatment in the eye bank before transplantation, leading to graft protection and prolonged graft survival.

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Suppressing Pro-Apoptotic Proteins by siRNA in Corneal Endothelial Cells Protects against Cell Death

Staehlke,

Mahajan,

Thieme

et al. 2024

Biomedicines

View full text Add to dashboard Cite

show abstract

Nanoparticle-Mediated Cell Delivery: Advancements in Corneal Endothelial Regeneration

Mallareddy,

Daigavane

2024

Cureus

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Corneal endothelial dysfunction poses significant challenges in ophthalmology, leading to corneal edema and vision loss. Traditional treatments, including corneal transplantation, are limited by donor scarcity and potential complications. Nanoparticle-mediated cell delivery emerges as a promising approach for corneal endothelial regeneration, offering targeted and minimally invasive solutions. This comprehensive review provides insights into the role of nanoparticles in enhancing cell survival, integration, and therapeutic efficacy. We discuss the current understanding of corneal endothelial dysfunction, emphasizing the importance of regeneration. Furthermore, we explore the potential implications of nanoparticle-mediated approaches in clinical practice, highlighting opportunities for personalized treatment strategies. Future directions are also discussed, including optimization of nanoparticle design and exploration of combination therapies. Overall, this review elucidates the promising advancements in nanoparticle-mediated cell delivery for corneal endothelial regeneration and underscores the importance of continued research efforts in this evolving field.

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Fusogenic liposome-coated nanoparticles for rapid internalization into donor corneal endothelial tissue to enable prophylaxis before transplantation

Abstract: Fusogenic liposome-coated nanoparticles rapidly internalize into ex vivo donor corneal endothelium within 3 hours of incubation at physiological temperature possibly via the non-endocytic, membrane fusion mechanism. Scale bar is 10 μm

Cited by 2 publications

References 54 publications

Suppressing Pro-Apoptotic Proteins by siRNA in Corneal Endothelial Cells Protects against Cell Death

Suppressing Pro-Apoptotic Proteins by siRNA in Corneal Endothelial Cells Protects against Cell Death

Nanoparticle-Mediated Cell Delivery: Advancements in Corneal Endothelial Regeneration

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