Fusobacterium spp. and colorectal cancer: cause or consequence?

Keku, Temitope O.; McCoy, Amber N.; Azcárate-Peril, M. Andrea

doi:10.1016/j.tim.2013.08.004

Cited by 65 publications

(45 citation statements)

References 10 publications

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“…pylori , E . coli , Fusobacterium , and Enterobacteriaceae have been implicated in colorectal carcinoma [38, 39]. Among microaerophilic bacteria, members of the genus Helicobacter are the most interesting, because they have been implicated in a variety of GI diseases including peptic ulcer, gastric cancer, and inflammatory bowel disease in humans and animals [32, 35, 37, 40].…”

Section: Discussionmentioning

confidence: 99%

Chronic Opisthorchis viverrini Infection Changes the Liver Microbiome and Promotes Helicobacter Growth

Itthitaetrakool

Pinlaor

et al. 2016

PLoS ONE

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Adults of Opisthorchis viverrini reside in the biliary system, inducing inflammation of bile ducts and cholangitis, leading to hepatobiliary disease (HBD) including cholangiocarcinoma. O. viverrini infection also has major implications for the bacterial community in bile ducts and liver. To investigate this in chronic O. viverrini infection (≥ 8 months p.i.), bacterial genomic DNA from livers of hamsters and from worms was investigated using culture techniques, PCR for Helicobacter spp. and high-throughput next-generation sequencing targeting the V3-V4 hypervariable regions of prokaryotic 16S rRNA gene. Of a total of 855,046 DNA sequence reads, 417,953 were useable after filtering. Metagenomic analyses assigned these to 93 operational taxonomic units (OTUs) consisting of 80 OTUs of bacteria, including 6 phyla and 42 genera. In the chronic O. viverrini-infected group, bacterial community composition and diversity were significantly increased compared to controls. Sequences of Fusobacterium spp. were the most common (13.81%), followed by Streptococcus luteciae (10.76%), Escherichia coli (10.18%), and Bifidobacterium spp. (0.58%). In addition, Helicobacter pylori (0.17% of sequences) was also identified in the liver of chronic O. viverrini infections, but not in normal liver. The presence of H. pylori was confirmed by PCR and by use of an antibody against bacterial antigen, supporting the metagenomics data. The identities of bacteria cultured for enrichment suggested that chronic O. viverrini infection changes the liver microbiome and promotes Helicobacter spp. growth. There may be synergy between O. viverrini and the liver microbiome in enhancing immune response-mediated hepatobiliary diseases.

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Section: Discussionmentioning

confidence: 99%

Chronic Opisthorchis viverrini Infection Changes the Liver Microbiome and Promotes Helicobacter Growth

Itthitaetrakool

Pinlaor

et al. 2016

PLoS ONE

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“…␤-Catenin then translocates into the nucleus and stimulates the lymphocyte enhancer factor (LEF), T-cell factor (TCF), and NF-B oncogenes. This bacterium invades the cell via internalization of E-cadherin by clathrin; stimulates the interleukin-10 (IL-10), IL-8, IL-6, IL-1␤, tumor necrosis factor alpha (TNF-␣), and COX-2 inflammatory genes; and ultimately induces tumorigenesis (40,41). Prevotella can adhere to epithelial cell lamellipodia and construct bacterial biofilm via its special affinity (42).…”

Section: Discussionmentioning

confidence: 99%

Microbiota in the Throat and Risk Factors for Laryngeal Carcinoma

Gong

Shi

Zhou

et al. 2014

Appl Environ Microbiol

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The compositions and abundances of the microbiota in the ecological niche of the human throat and the possible relationship between the microbiota and laryngeal cancer are poorly understood. To obtain insight into this, we enrolled 27 laryngeal carcinoma patients and 28 subjects with vocal cord polyps as controls. For each subject, we simultaneously collected swab samples from the upper throat near the epiglottis (site I) and tissue samples from the vestibulum laryngis to the subglottic region (site II). The microbiota of the throat were fully characterized by pyrosequencing of barcoded 16S rRNA genes. We found 14 phyla, 20 classes, 38 orders, 85 families, and 218 genera in the throats of enrolled subjects. The main phyla were Firmicutes (54.7%), Fusobacteria (14.8%), Bacteroidetes (12.7%), and Proteobacteria (10.6%). Streptococcus (37.3%), Fusobacterium (11.3%), and Prevotella (10.6%) were identified as the three most predominant genera in the throat. The relative abundances of 23 bacterial genera in site I were significantly different from those in site II (P < 0.05). The relative proportions of 12 genera largely varied between laryngeal cancer patients and control subjects (P < 0.05). Collectively, this study outlined the spatial structure of microbial communities in the human throat. The spatial structure of bacterial communities significantly varied in two anatomical sites of the throat. The bacterial profiles of the throat of laryngeal cancer patients were strongly different from those of control subjects, and several of these microorganisms may be related to laryngeal carcinoma.

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“…As in APO, F. nucleatum is often detected in conjunction with other oral species, suggesting an oral source of infection [71]. The question arises whether F. nucleatum is a passenger or a driver of colorectal tumorigenesis [72,73]. Rubinstein et al demonstrate that F. nucleatum stimulates CRC cancer growth by modulating the E-cadherin/β catenin signaling via its unique FadA adheisn (see below).…”

Section: Diseases Implicating F Nucleatummentioning

confidence: 99%