Fusobacterium periodonticum, A New Species from the Human Oral Cavity

Slots, J; Potts, Thomas V.; Mashimo, Paul A.

doi:10.1177/00220345830620090901

Cited by 52 publications

(20 citation statements)

References 12 publications

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“…Fusobacteria are Gram negative, anaerobic, nonmotile and nonsporulating [9]. Fusobacterium nucleatum is the type species of this genus and the most frequently isolated from humans [10], and Fusobacterium periodonticum was first isolated in 1983 from the human oral cavity [11]. However, our strain did not fulfil the usual criteria to be assigned to a previously known species of this genus.…”

Section: Introductionmentioning

confidence: 80%

Noncontiguous finished genome sequence and description of Fusobacterium massiliense sp. nov. isolated from human duodenum

Mailhe

Ricaboni

Vitton

et al. 2017

New Microbes and New Infections

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The strain Marseille-P2749T (= CSUR P2749 = DSM 103085) was isolated as part of culturomics study from a liquid duodenum sample from a French man. Bacterial cells were Gram-negative bacilli, fusiform shaped and non–spore forming, and they grew in microaerophilic and anaerobic atmosphere. Its genome is 1 809 169 bp long and contains 1646 protein-coding genes. The DNA G+C content was 27.33 mol%. This strain exhibited a 95.9% sequence similarity with Fusobacterium periodonticum, the phylogenetically closest species with standing in nomenclature. Strain Marseille-P2749T is suggested to be a novel species belonging to the genus Fusobacterium, for which the name Fusobacterium massiliense sp. nov. is proposed.

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Section: Introductionmentioning

confidence: 80%

Noncontiguous finished genome sequence and description of Fusobacterium massiliense sp. nov. isolated from human duodenum

Mailhe

Ricaboni

Vitton

et al. 2017

New Microbes and New Infections

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“…A number of newly described species have been isolated from human periodontal lesions. These include B. forsythus (20), Centipeda periodontii (8), Eubacterium timidum, Eubacterium nodatum and Eubacterium brachy (13), Eubacterium yurii (12), Fusobacterium periodonticum (16), Wolinella recta, Campylobacter concisus, Bacteroides gracilis (7,18), and Wolinella curva (19). The exact role of these bacteria in the etiology and pathogenesis of periodontal disease still needs to be clarified.…”

Section: Discussionmentioning

confidence: 99%

Bacteroides forsythus in adult gingivitis and periodontitis

Lai

Listgarten

et al. 1987

Oral Microbiology and Immunology

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107

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Bacteroides forsythus, a newly named species from the human mouth, possesses a distinct cell wall ultrastructure and a unique set of cell surface antigens. To determine the distribution of B. forsythus in the periodontal region, supragingival plaque was collected from 16 healthy adults and from 11 adults with mild gingivitis, 12 with severe gingivitis and 17 with periodontitis. Subgingival samples from 27 diseased sites were examined as well. B. forsythus was located in plaque smears by indirect immunofluorescence microscopy. Rabbit antibody to B. forsythus strain FDC 335 constituted the primary antibody and fluoresceinlabelled goat‐antirabbit antibody the secondary antibody. There was a significant difference (p<0.05) between the proportions of B. forsythus in supragingival samples of healthy subjects (0.2%) vs. individuals with mild gingivitis (1.3%), severe gingivitis (1.0%) and adult periodontitis (0.9%). A significant difference (p< 0.001) in B. forsythus proportions was also detected between supragingival (0.9%) and subgingival (15.3%) samples from adult periodontitis patients. In a separate study, patients previously treated for moderate to severe adult periodontitis were monitored over a 12‐month period for evidence of disease recurrence. A significant increase in the proportions of B. forsythus was found at sites with breakdown as compared to stable sites. Breakdown was defined as loss of attachment of 2 mm or more from base line, as measured from a reference stent, or a probing depth increase of 3 mm or more. These results support the assertion that B. forsythus is associated with advanced periodontitis as well as recurrent periodontitis. Monitoring the subgingival proportions of B. forsythus may be of diagnostic value in patients on maintenance therapy. Whether B. forsythus causes periodontal disease or is a secondary colonizer of periodontal lesions remains to be determined.

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“…For example, of the ϳ16 species comprising the genus Fusobacterium (1,5,6,9,32,48), only F. mortiferum has the capacity to utilize such an extraordinarily wide variety of carbohydrates (monosaccharides, disaccharides, and both ␣-and ␤-glycosides) as energy sources. One wonders whether F. mortiferum has acquired the necessary genetic information for the transport and dissimilation of these carbohydrates from other bacterial species.…”

Section: Discussionmentioning

confidence: 99%

6-phospho-alpha-D-glucosidase from Fusobacterium mortiferum: cloning, expression, and assignment to family 4 of the glycosylhydrolases

Bouma

Reizer

et al. 1997

J Bacteriol

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The Fusobacterium mortiferum malH gene, encoding 6-phospho-␣-glucosidase (maltose 6-phosphate hydrolase; EC 3.2.1.122), has been isolated, characterized, and expressed in Escherichia coli. The relative molecular weight of the polypeptide encoded by malH (441 residues; M r of 49,718) was in agreement with the estimated value (ϳ49,000) obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the enzyme purified from F. mortiferum. The N-terminal sequence of the MalH protein obtained by Edman degradation corresponded to the first 32 amino acids deduced from the malH sequence. The enzyme produced by the strain carrying the cloned malH gene cleaved [U- Members of the genus Fusobacterium are gram-negative obligate anaerobes that colonize the oral and gastrointestinal tracts of humans and animals (5,24,27,31). The frequent isolation of these species from abscesses and necrotic tissues indicates their causative or contributory roles in a variety of infections (6,7,15,16,25). The prevalence and increased numbers of certain species (e.g., Fusobacterium nucleatum) in patients with gingivitis and periodontitis suggest that this organism may play a role in the etiology of these oral diseases (6,32,50). Although the pathogenic potential and medical importance of fusobacteria are widely recognized, surprisingly little is known about the biochemistry, genetics, or regulation of energy generation in these species.Most species of fusobacteria derive energy for growth via fermentation of amino acids such as glutamate, lysine, and serine (4, 10, 11, 17). Until recently, members of the genus were usually described as asaccharolytic or weakly fermentative (24,31,42). In this context, it was of considerable interest to discover that Fusobacterium mortiferum metabolized a wide variety of carbohydrates, including monosaccharides and both ␣-and ␤-glycosides, as energy sources (40,41,54,55). These compounds are transported by the phosphoenolpyruvate-dependent sugar:phosphotransferase system (PEP:PTS) (29,35,43). This multicomponent group translocation system comprises both membrane-associated enzymes and cytoplasmic proteins. In concert, the PEP:PTS facilitates the simultaneous translocation, phosphorylation, and internalization of sugars by the cell. A functional PEP:PTS requires two general proteins, designated enzyme I and HPr, that are allied with sugarspecific complexes comprising enzymes IIA, IIB, and IIC (for discussions, see references 35 and 44) to effect the sequential transfer of the high-energy phosphoryl moiety from PEP to the incoming sugar.PEP-dependent PTS have been reported for all major groups of carbohydrates in both gram-negative (29, 35) and gram-positive (19, 39, 51, 52) bacteria. However, the existence of a maltose-specific PTS has been a source of controversy, and evidence has been presented both for and against this postulate (for discussions, see references 40 and 53). In our laboratory, studies of maltose utilization by F. mortiferum resulted in the serendipitous isolation of maltose 6-phospha...

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Fusobacterium periodonticum, A New Species from the Human Oral Cavity

Cited by 52 publications

References 12 publications

Noncontiguous finished genome sequence and description of Fusobacterium massiliense sp. nov. isolated from human duodenum

Noncontiguous finished genome sequence and description of Fusobacterium massiliense sp. nov. isolated from human duodenum

Bacteroides forsythus in adult gingivitis and periodontitis

6-phospho-alpha-D-glucosidase from Fusobacterium mortiferum: cloning, expression, and assignment to family 4 of the glycosylhydrolases

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