Fusion with Activated Mouse Oocytes Modulates the Transcriptional Activity of Introduced Somatic Cell Nuclei

Borsuk, Ewa; Szöllösi, Maria S.; Besombes, Didier; Debey, Pascale

doi:10.1006/excr.1996.0160

Cited by 17 publications

(29 citation statements)

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“…After introduction into the cytoplasm of MII oocytes and early parthenogenotes these nuclei undergo morphological and physiological changes, which have been described in details in several papers. [72][73][74][75][76] Thymocyte nuclei in ovulated oocytes. Thymocyte nuclei introduced into the cytoplasm of ovulated oocytes underwent premature chromosome condensation (PCC).…”

Section: Resultsmentioning

confidence: 99%

Phosphorylation of histone H3 serine 10 in early mouse embryos: Active phosphorylation at late S phase and differential effects of ZM447439 on first two embryonic mitoses

Teperek¹,

Meglicki²,

Pasternak³

et al. 2010

Cell Cycle

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Section: Resultsmentioning

confidence: 99%

Phosphorylation of histone H3 serine 10 in early mouse embryos: Active phosphorylation at late S phase and differential effects of ZM447439 on first two embryonic mitoses

Teperek¹,

Meglicki²,

Pasternak³

et al. 2010

Cell Cycle

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“…3). It has been known from previous reports (Szo Èllo Èsi et al, 1986(Szo Èllo Èsi et al, , 1988(Szo Èllo Èsi et al, , 1998Czoøowska et al, 1992;Borsuk et al, 1996) that somatic and embryonic cell nuclei introduced into mouse oocytes undergo morphological and functional changes depending on whether the breakdown of their nuclear membrane occurred (metaphase II recipient oocytes) or not (activated recipient oocytes). As was expected for variant I, 3T3 nuclei fused to already activated eggs did not reveal remarkable changes in their original size, shape, and chromatin structure and continued BrUTP incorporation (30/30) when examined 2±4 hr after transfer into the egg (Fig.…”

Section: Ef®ciency Of Electropermeabilization Formentioning

confidence: 99%

Electric field-mediated BrUTP uptake by mouse oocytes, eggs, and embryos

Waksmundzka¹,

Debey²

2000

Mol. Reprod. Dev.

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“…The most important lessons learned from these studies were that remodelling requires short (even very short) loss of the nuclear membrane and exposure to the cytoplasm of oocyte that had earlier undergone GVBD, and that in the activated oocyte the conditions that allow the denudation of the foreign nucleus disappear very quickly Szollosi et al, 1986Szollosi et al, , 1988Szollosi et al, , 1998Czolowska et al, 1984Czolowska et al, , 1992. Later studies were concentrated on the functional aspects of remodelling: transcription and DNA replication (Borsuk et al, 1996;Szollosi et al, 1998;Borsuk and Maleszewski, 2002).…”

Section: Cloningmentioning

confidence: 99%

“…Our results showing that the cytoplasm of G1 parthenogenetic one-cell embryo has the ability to support the RNA synthesis in the foreign nucleus, introduced by the means of cell fusion, contradict this theory. Nuclei of transcribing somatic cells (murine erytroblastoma cells, MEL) continue transcription and those, which were transcriptionally inactive (thymocyte nuclei), restart it, and remain active until the end of the cell cycle (Borsuk et al, 1996;Borsuk and Maleszewski, 2002). These results suggest, that the cytoplasm of the early parthenogenetic one-cell embryo is transcriptionally permissive, but its genome is not ready for the initiation of RNA synthesis.…”

Section: Regulation Of Transcription and Dna Replicationmentioning

confidence: 99%

Mammalian and avian embryology at Warsaw University (Poland) from XIX century to the present

Tarkowski

Maleszewski²,

Rogulska³

et al. 2008

Int. J. Dev. Biol.

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KEY WORDS: mouse, regulative development, chimaerism, cell lineage, fertilization The beginnings: XIX century -1939Poland lost independence in the XVIII century after she was partitioned between Russia, Prussia and Austria from 1772 to 1795. In the times that followed, many Polish scientists studied and often lived abroad, and many foreigners came to work in Polish academic institutions. In 1816 Alexander I, the Emperor of Russia and the usurper King of Poland, granted permission for the establishment of Warsaw University. The University followed the tradition of three earlier institutions of higher education: Collegium Nobilium, opened in 1766, the School of Law (founded in 1808) and the School of Medicine (founded in 1809). The University still bears the emblem described as "an ancient Polish eagle with a crown, with spread wings, a palm frond in its left claws and laurel branch in its right, the symbols of patient diligence and honour, surrounded by five stars representing its five academic faculties". These faculties were theology, law, medicine, philosophy, and the liberal arts. After the fall of the November 1830 uprising, in which a majority of students had participated, the University was closed in 1831. In 1857 there was a brief period of Int. J. Dev. Biol. 52: 121-134 (2008) +48-22-55-41-210. e-mail: akt@biol.uw.edu.pl # Note: This article is not a review of the principle interests of this Department, and therefore, we quote other authors very selectively, for instance, when they carried out concurrently similar studies, or when their studies are essential for the understanding of the rationale and/or implications of our experiments. liberalization that resulted in the opening of the Main School (Szkola Glowna) with the departments of medical-surgical, law and administration, philological-historical, and mathematics-physics. The Main School was closed in 1869, on the wave of increasing oppression and russification after the fall of the second, the January 1863 uprising. However, in the short period of the existence of the Main School as many as three thousand students graduated and the School had great impact on the development of Polish science and culture. In 1870, the Russian Imperial University was opened in Warsaw. The majority of faculty teachers were Russians and the official (teaching) language was Russian. Steadily the University expanded: new buildings were constructed; academic potential increased and precious zoological and botanical collections were created.The Department of Comparative Anatomy was founded in 1870. It was headed by Russian scientists: Mitrofan Ganin (1870-84), Mitrofan Uljanin (1884-1888) and Pawel Mitrofanow (Mitrophanow) . Mitrofanow ( Fig. 1) was an outstanding avian embryologist working mainly on early embryos; in papers published in 1895 and 1899, he presented evidence that 122 A. Tarkowski et al. which in 1918 -when Poland regained independencebecame one of the first legally functioning Polish national institutions. Soon, many distinguished specialists and...

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Fusion with Activated Mouse Oocytes Modulates the Transcriptional Activity of Introduced Somatic Cell Nuclei

Cited by 17 publications

References 0 publications

Phosphorylation of histone H3 serine 10 in early mouse embryos: Active phosphorylation at late S phase and differential effects of ZM447439 on first two embryonic mitoses

Phosphorylation of histone H3 serine 10 in early mouse embryos: Active phosphorylation at late S phase and differential effects of ZM447439 on first two embryonic mitoses

Electric field-mediated BrUTP uptake by mouse oocytes, eggs, and embryos

Mammalian and avian embryology at Warsaw University (Poland) from XIX century to the present

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