Fusion of simian immunodeficiency virus with liposomes and erythrocyte ghost membranes: effects of lipid composition, pH and calcium

Larsen, Charles E.; Alford, Dennis R.; Young, Lawrence J.T.; McGraw, Thomas P.; Düzgüneş, Nejat

doi:10.1099/0022-1317-71-9-1947

Cited by 25 publications

(10 citation statements)

References 67 publications

(83 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All the assays performed with liposomes must take into account the limitations imposed by these studies. In this regard, HIV and simian immunodeficiency virus, which are known to enter CD4 + cells by fusing with the plasma membrane at neutral pH, have significantly enhanced fusogenic properties when interacting with liposomes at acidic pH (Larsen et al, 1990(Larsen et al, , 1993. The physiological significance of this increased fusogenic capacity is doubtful.…”

Section: Discussionmentioning

confidence: 93%

Phospholipid interactions of the putative fusion peptide of hepatitis B virus surface antigen S protein

Rodrı́guez-Crespo

Núñez

Gómez-Gutiérrez

et al. 1995

Journal of General Virology

View full text Add to dashboard Cite

One of the first steps in the infective cycle of an enveloped virus consists of the fusion of the viral and cellular membranes. This process is usually achieved as a result of membrane destabilization brought about by a viral fusion peptide located at the amino terminus of one of the viral envelope glycoproteins. Previous sequence similarity studies by Rodrlguez-Crespo et al. (Journal of General Virology 75, 637-639, 1994) have shown that a hydrophobic stretch in the amino-terminal sequence of the S protein of hepatitis B virus shares several characteristics with fusion peptides of retroviruses and paramyxoviruses. A 16 residue peptide with this sequence was synthesized and its interaction with liposomes characterized. This peptide was able to mediate vesicle aggregation, lipid mixing and liposome leakage in a pH dependent manner at concentrations ranging from 3"5 to 52.0 pM. These effects were specific for negatively charged phospholipid vesicles. The peptide was also able to haemolyse erythrocytes. This study supports the notion that the sequence might be important in the initial infective steps of this virus, interacting with the target membranes and bringing about their subsequent destabilization.

show abstract

Section: Discussionmentioning

confidence: 93%

Phospholipid interactions of the putative fusion peptide of hepatitis B virus surface antigen S protein

Rodrı́guez-Crespo

Núñez

Gómez-Gutiérrez

et al. 1995

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The cells were split 1 : 6 and fresh medium was added every 3 to 4 days. HIV-1 LAy LA~ (formerly the LAV-BRU strain) (WainHobson et al, 1991), initially provided by F. Barr6-Sinoussi (Pasteur Institute, Paris, France), was propagated in human lymphoblastoid leukemia (CEM) cells, and purified as described by Larsen et al (1990) and stored at -70 °C. The purified HIV-1 was propagated in A3.01 cells and designated HIV-1 m~u.…”

Section: Methodsmentioning

confidence: 99%

A monoclonal antibody to the gp120-CD4 complex has differential effects on HIV-induced syncytium formation and viral infectivity

Konopka

Pretzer²,

Celada³

et al. 1995

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

A murine monoclonal antibody (MAb F-91-55) raised against the complex of soluble CD4 and human immunodeficiency virus type 1 (HIV-1) gpl20 had previously been found to inhibit syncytium formation without inhibiting the interaction of CD4 with gpl20, and its binding site was localized within the first two domains (D 1/D2) of CD4. We investigated whether this antibody inhibited the infectivity of HIV-1 in the CD4 + T cell lines A3.01, Sup-T1 and H9. We also examined the effect of the antibody on syncytium formation between these cells and chronically infected H9 cells. Syncytium formation was found to depend critically on the incubation medium used. The effect of the MAb on HIV-1 infectivity was very limited with A3.01 and Sup-TI cells, although it inhibited syncytium formation between A3.01 or Sup-T1 and chronically infected H9 cells. In contrast, the MAb inhibited significantly the infectivity of HIV-1 in H9 cells, but it also inhibited syncytium formation between H9 and chronically infected H9 cells to a greater extent than in the case of the other cell lines. Our results indicate that cellular systems used for syncytium assays differ in their susceptibility to inhibitory antibodies. In the A3.01 and Sup-T1 cell systems, the differences in the ability of the MAb to block viral entry or syncytium formation raise the possibility that the mechanisms of interaction of gp 120/ gp41 with cell membrane CD4 may be different in cell-cell and virus-cell membrane fusion.

show abstract

“…For each experiment 50 or 100 gg total viral protein was thawed and the viral envelope was labelled with R18 as reported previously (Konopka et al, 1990;Larsen et al, 1990). Briefly, the virus was incubated with an ethanolic solution of R18 (final ethanol concentration of less than 1% v/v) for 60 rain at room temperature.…”

Section: Cells A301 Cells Were Obtained From T Folks (Centers For mentioning

confidence: 99%

Simian immunodeficiency virus (SIVmac251) membrane lipid mixing with human CD4+ and CD4- cell lines in vitro does not necessarily result in internalization of the viral core proteins and productive infection

Stamatatos

Düzgüneş

1993

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

Fusion of simian immunodeficiency virus with liposomes and erythrocyte ghost membranes: effects of lipid composition, pH and calcium

Cited by 25 publications

References 67 publications

Phospholipid interactions of the putative fusion peptide of hepatitis B virus surface antigen S protein

Phospholipid interactions of the putative fusion peptide of hepatitis B virus surface antigen S protein

A monoclonal antibody to the gp120-CD4 complex has differential effects on HIV-induced syncytium formation and viral infectivity

Simian immunodeficiency virus (SIVmac251) membrane lipid mixing with human CD4+ and CD4- cell lines in vitro does not necessarily result in internalization of the viral core proteins and productive infection

Contact Info

Product

Resources

About