Fusion of Intact Human Erythrocytes and Erythrocyte Ghosts

Peretz, Hava; Toister, Z.; Laster, Yehudith; Loyter, Abraham

doi:10.1083/jcb.63.1.1

Cited by 128 publications

(63 citation statements)

References 24 publications

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“…In the first place a membranous enzyme activated by Ca 2+ may regulate entry of material into the lysosome. In view of the widely observed stimulation of fusion by Ca 2+ [7,25,28,30,32,33,34,38,47,48] one can consider the possibility that the ATPase regulates the free Ca 2+ concentration, e.g., by pumping Ca 2+ into the lysosome. Thus the ATPase may function in the membrane fusion process by means of which material from endocytotic vesicles and autophagic vacuoles is taken into lysosomes for digestion.…”

Section: Discussionmentioning

confidence: 99%

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Membranous localization and properties of ATPase of rat liver lysosomes

Schneider

1977

J. Membrain Biol.

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Lysosomes isolated from rat liver were found to have ATPase activity (EC No 3.6.1.3). Subfractionation of the lysosomes revealed a membranous localization of ATPase activity. The enzyme has half maximal activity at 0.2 mM ATP and is inhibited by high concentrations of ATP. The apparent Km for divalent metal is 0.2 mM, and either Ca2+ or Mg2+ give maximal activity. The ATPase activity has latency when lysosomes are isolated from rats treated with Triton WR-1339. This latency may be due to the presence of internalized sucrose because the activity of L fraction lysosomes is much less latent and Triton WR-1339 itself is not inhibitory. The latency of glucosaminidase, a marker enzyme for lysosomes, contrasts with the low latency of the ATPase and points to an ATPase with an exposed active site in intact lysosomes.

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Section: Discussionmentioning

confidence: 99%

“…tosis [7,33,34,38,47,48], the bile-canalicular face of the plasma membrane may. also have need of a Ca 2 +-ATPase.…”

Section: A Tpase Activity Associated With Lysosomesmentioning

confidence: 99%

Membranous localization and properties of ATPase of rat liver lysosomes

Schneider

1977

J. Membrain Biol.

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“…Among these agents are inactivated Sendai virus [3,9,14,17,19,20,26] [5, 6, 18, 19, 23 25]. Furthermore, it appears these agents promote fusion of natural and artificial membranes both with themselves and with each other.…”

mentioning

confidence: 99%

Fusion of photoreceptor membrane vesicles

1979

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The n-alkyl bromides with 6 to 10 carbons induce formation of vesicles of 5 to 100 micrometer diameter from the small vesicles (0.1 micrometer average diameter) produced by disruption of the discs from frog rod photoreceptors. The n-alkanes, n-alkyl iodides and n-alkyl chlorides are relatively ineffective. The formation of large vesicles is independent of calcium concentration and is distinguished from fusion processes previously reported by the large number of vesicles involved. The results reported here together with others suggest the occurrence of multiple fusion (and/or rupture-resealing) events between vesicles, induced by the n-alkyl bromides.

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“…Serum amine oxidase was purified according to Mondovi et al (1983) and its activity (50 U/mg) was determined by the benzylamine method (Tabor et al, 1955 Sendai virus was isolated from the allantoic fluid of Fusion of loaded RSVE with cultured cells fertilized chicken eggs. Its hemagglutination units were determined as described (Peretz et al, 1974).…”

mentioning

confidence: 99%

Fusion‐mediated microinjection of active amine and diamine oxidases into cultured cells: Effect on protein and DNA synthesis in chick embryo fibroblasts and in glioma cells

Bachrach

Ash

Abu-Elheiga

et al. 1987

Journal Cellular Physiology

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Serum amine oxidase and/or porcine kidney diamine oxidase were trapped within reconstituted Sendai virus envelopes, and retained their activity. The trapped enzymes that were detected by radioimmunoblots were microinjected into cultured cells by fusion. When diamine oxidase was microinjected into cultured fibroblasts of chick or rat embryos, a temporary arrest in protein and DNA synthesis was observed. The inhibitory effect was more significant when both serum amine oxidase and kidney diamine oxidase were microinjected into those cultured cells. Fibroblasts of either chick or rat embryos transformed by Rous sarcoma virus were more susceptible to the injected enzymes than the normal cultures, showing a complete arrest in protein and DNA synthesis within 4 hours. Similar results were obtained by microinjecting diamine oxidase into cultured glioma cells. The injected enzyme catalyzed the oxidation of intracellular polyamines. The resulting oxidation product (hydrogen peroxide and aminoaldehydes) apparently caused the arrest in the synthesis of macromolecules.

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Fusion of Intact Human Erythrocytes and Erythrocyte Ghosts

Cited by 128 publications

References 24 publications

Membranous localization and properties of ATPase of rat liver lysosomes

Membranous localization and properties of ATPase of rat liver lysosomes

Fusion of photoreceptor membrane vesicles

Fusion‐mediated microinjection of active amine and diamine oxidases into cultured cells: Effect on protein and DNA synthesis in chick embryo fibroblasts and in glioma cells

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