Fusion of Ginseng Farnesyl Diphosphate Synthase and &lt;i&gt;Centella asciatica&lt;/i&gt; Squalene Synthase Involved in Triterpenoid Biosynthesis

Jung, Su Jin; Kim, Young Chang; Jin, Mei Lan; Jetter, Reinhard; Kim, Ok-Tae

doi:10.18520/cs/v113/i04/785-790

Cited by 2 publications

(1 citation statement)

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“…In Centella asiatica hairy roots, there was no correlation between the expression of SS and FPS genes [ 20 ]. However, to improve triterpenoid biosynthesis, a fusion protein, as a valuable tool including very high activities of both FPS and SS , was constructed and expressed in Escherichia coli , which suggests that further research about the positive correlation between expression of PnSS and AeFPS gene has great potential [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Expression of PnSS Promotes Squalene and Oleanolic Acid (OA) Accumulation in Aralia elata via Methyl Jasmonate (MeJA) Induction

Dai

Xia

et al. 2023

Genes

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Aralia elata is an important herb due to the abundance of pentacyclic triterpenoid saponins whose important precursors are squalene and OA. Here, we found that MeJA treatment promoted both precursors accumulation, especially the latter, in transgenic A. elata, overexpressing a squalene synthase gene from Panax notoginseng(PnSS). In this study, Rhizobium-mediated transformation was used to express the PnSS gene. Gene expression analysis and high-performance liquid chromatography (HPLC) were used to identify the effect of MeJA on squalene and OA accumulation. The PnSS gene was isolated and expressed in A. elata. Transgenic lines showed a very high expression of the PnSS gene and farnesyl diphosphate synthase gene (AeFPS) and a slightly higher squalene content than the wild-type, but endogenous squalene synthase (AeSS), squalene epoxidase (AeSE), and β-amyrin synthase (Aeβ-AS) gene were decreased as well as OA content. Following one day of MeJA treatment, the expression levels of PeSS, AeSS, and AeSE genes increased significantly. On day 3, the maximum content of both products reached 17.34 and 0.70 mg·g−1, which increased 1.39- and 4.90-fold than in the same lines without treatment. Transgenic lines expressing PnSS gene had a limited capability to promote squalene and OA accumulation. MeJA strongly activated their biosynthesis pathways, leading to enhance yield.

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Section: Discussionmentioning

confidence: 99%