Fusion between myogenic cells in Vivo: An ultrastructural study in regenerating murine skeletal muscle

Robertson, Terry; Grounds, Miranda D.; Mitchell, Christopher A.; Papadimitriou, J. M.

doi:10.1016/1047-8477(90)90111-o

Cited by 55 publications

(30 citation statements)

References 24 publications

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“…The process of fusion was observed in the developing esophagus through the occurrence of intercommunicating pores within a pair of blind pouches formed by the membranes of both cells (Lipton and Konigsberg, 1972;Horsley and Pavlath, 2004). Fusion between MTs, which is described in skeletal myogenesis and in regenerating murine skeletal muscle (Rash and Fambrough, 1973;Robertson et al, 1990), was not seen in the present study.…”

Section: Esophageal Striated Myogenesiscontrasting

confidence: 43%

Ultrastructural analysis of the smooth‐to‐striated transition zone in the developing mouse esophagus: Emphasis on apoptosis of smooth and origin and differentiation of striated muscle cells

Wörl

Neuhuber

2005

Developmental Dynamics

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The exact mechanism of smooth-to-striated muscle conversion in the mouse esophagus is controversial. Smooth-to-striated muscle cell transdifferentiation vs. distinct differentiation pathways for both muscle types were proposed. Main arguments for transdifferentiation were the failure to detect apoptotic smooth and the unknown origin of striated muscle cells during esophageal myogenesis. To reinvestigate this issue, we analyzed esophagi of 4-day-old mice by electron microscopy and a fine-grained sampling strategy considering that, in perinatal esophagus, the replacement of smooth by striated muscle progresses craniocaudally, while striated myogenesis advances caudocranially. We found numerous (1) apoptotic smooth muscle cells located mainly in a transition zone, where smooth intermingled with developing striated muscle cells, and (2) mesenchymal cells in the smooth muscle portion below the transition zone, which appeared to give rise to striated muscle fibers. Taken together, these results provide further evidence for distinct differentiation pathways of both muscle types during esophagus development. Developmental Dynamics 233:964 -982, 2005.

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Section: Esophageal Striated Myogenesiscontrasting

confidence: 43%

Ultrastructural analysis of the smooth‐to‐striated transition zone in the developing mouse esophagus: Emphasis on apoptosis of smooth and origin and differentiation of striated muscle cells

Wörl

Neuhuber

2005

Developmental Dynamics

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“…The fusion between myotubes in vitro has previously been reported 31,32 , and it has also been suggested that some myofibres could fuse one to another in vivo during regeneration 33 . In our conditions, the fusion of two normal myotubes in vitro was a rare event as shown by videomicroscopy.…”

Section: Discussionmentioning

confidence: 84%

Normal muscle regeneration requires tight control of muscle cell fusion by tetraspanins CD9 and CD81

et al. 2013

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Skeletal muscle regeneration after injury follows a remarkable sequence of synchronized events. However, the mechanisms regulating the typical organization of the regenerating muscle at different stages remain largely unknown. Here we show that muscle regeneration in mice lacking either CD9 or CD81 is abnormal and characterized by the formation of discrete giant dystrophic myofibres, which form more quickly in the absence of both tetraspanins. We also show that, in myoblasts, these two tetraspanins associate with the immunoglobulin domain molecule CD9P-1 (EWI-F/FPRP), and that grafting of CD9P-1-depleted myoblasts in regenerating muscles also leads to abnormal regeneration. In vitro myotubes lacking CD9P-1 or both CD9 and CD81 fuse with a higher frequency than normal myotubes. Our study unveils a mechanism preventing inappropriate fusion of myotubes that has an important role in the restitution of normal muscle architecture during muscle regeneration.

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“…Myoblasts were identified as small rounded or elongated cells with single, central nuclei, free ribosomes, Golgi, short cisternae of rough endoplasmic reticulum, a large nucleus-to-cytoplasm ratio, and few mitochondria with or without early deposition of contractile filaments and Z-disk material. 56 Nuclei of myoblasts were photographed at high magnification (greater than 28,000×) using a Phillips 300 TEM. Coded micrographs were evaluated for the density of gold particles present over the nucleus (number of particles per unit area) and calculated as a labeling index (LI), since nuclear bFGF is related to cell proliferation.…”

mentioning

confidence: 99%

Dystrophy and myogenesis inmdx diaphragm muscle

et al. 1998

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Fusion between myogenic cells in Vivo: An ultrastructural study in regenerating murine skeletal muscle

Cited by 55 publications

References 24 publications

Ultrastructural analysis of the smooth‐to‐striated transition zone in the developing mouse esophagus: Emphasis on apoptosis of smooth and origin and differentiation of striated muscle cells

Ultrastructural analysis of the smooth‐to‐striated transition zone in the developing mouse esophagus: Emphasis on apoptosis of smooth and origin and differentiation of striated muscle cells

Normal muscle regeneration requires tight control of muscle cell fusion by tetraspanins CD9 and CD81

Dystrophy and myogenesis inmdx diaphragm muscle

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