Fusicoccins P and Q, and 3-Epifusicoccins H and Q, New Polar Fusicoccins from Isolate Niigata 2-A of a Peach Fusicoccum Canker Fungus

(+)-Menthol and Its Hydroxy Derivatives, Novel Fungal Monoterpenols from the Fusicoccin-producing Fungi,Phomopsis amygdaliF6a and Niigata 2

“…In our current eŠort to isolate new fusicoccins from the fungus, it was found that P. amygdali F6a 4) (the Italian strain) produced fusicoccin J together with new 16-O-demethylfusicoccin J, and that P. amygdali Niigata 2 5) (the Japanese strain) produced 3?-deacetylfusicoccin A together with new 3-epifusicoccins. 6) We also isolated (+)-menthol (1) as a novel fungal metabolite from the cultureˆltrate of the Niigata 2 strain, and its related metabolites, (+)-(1R,3S,4R,6S )-6-hydroxymenthol (3) as a new compound and (+)-(1S,3S,4R)-7-hydroxymenthol (2), and known 4 from a cultureˆltrate of the F6a strain (Fig. 1).…”

Novel Fusicoccins R and S, and the Fusicoccin S Aglycon (Phomopsiol) fromPhomopsis amygdaliNiigata 2-A, and Their Seed Germination-stimulating Activity in the Presence of Abscisic Acid

“…7) We found in this study that P. amygdali Niigata 2-A produced FC S of an unexpected 3 -hydroxyfusicoccin and phomopsiol of its aglycon, together with 3 -hydroxyfusicoccins of FC R and 3 -hydroxy-FC J. The isolation of FC S and phomopsiol from the fungus suggested the presence of an additional new 3 -hydroxylation route in the 3-hydroxylation pathway of fusicoccin biosynthesis (Fig.…”

“…6) We have reported in a previous paper the isolation of new polar fusicoccins P and Q, and 3-epifusicoccins H and Q from the 3 0 -deacetyl-FC A-producing fungus, Phomopsis amygdali Niigata 2-A (an isolate of a peach Fusicoccum canker fungus), together with the presence of two fusicoccin-biosynthetic pathways finally giving FC A and its 3 0 -deacetyl derivative, as well as 16-Odemethyl-3-epi-FC J. 7) We have recently found that cotylenin A (CN A, Fig. 1), 8) the sole fusicoccin-congener isolated as a potent plant-growth regulator from Cladosporium sp.…”

An Enzyme Catalyzing O‐Prenylation of the Glucose Moiety of Fusicoccin A, a Diterpene Glucoside Produced by the Fungus Phomopsis amygdali

“…Given the chemical structures of the compounds isolated from a culture broth of the FC producer, substrates of PAPT were expected to be FC Q [40] and/or FC P (Scheme 1). Since only FC P was available, it was tested as a substrate.…”

“…In vitro assay of PAPT: The assay mixture (100 mL) for PAPT contained FC P [40] or FC H (0.5 mm), [41] DMAPP (0.5 mm), Tris·HCl (50 mm, pH 7.5), and an appropriate amount of PAPT. This mixture was incubated at 30 8C for 2 h and the reaction was stopped by the addition of methanol (100 mL).…”