Fusibacter paucivorans gen. nov., sp. nov., an anaerobic, thiosulfate-reducing bacterium from an oil-producing well

Ravot, Gilles; Magot, Michel; Fardeau, Marie‐Laure; Patel, Bharat K. C.; Thomas, Pierre; Garcia, Jean‐Louis; Ollivier, Bernard

doi:10.1099/00207713-49-3-1141

Cited by 142 publications

(99 citation statements)

References 27 publications

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“…7C), suggesting that VFA (volatile fatty acid) components within the production water serve as their major carbon sources, and demonstrated a higher support between the phylogenetic data and the physicochemical measurements. Other sequence type within the phylum Firmicutes also had a greater proportion in high-temperature reservoirs, such as sequence type (S3B clone: 98), most closely related to Fusibacter spp., an anaerobic, thiosulfate-reducing bacterium isolated from an oil-producing well (Ravot et al, 1999). It is not a typical thermophilic microorganism with optimal growth temperature at 37 • C, and such types of sequences were also found in low-temperature petroleum reservoirs, represented as (S6 B clone: 70) with petroleum temperature at 20 • C. CCA analysis showed that the phylum Thermotogae is another bacterial group correlated positively with the temperature.…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of the microbial community structures in water-flooding petroleum reservoirs with different temperatures

et al. 2012

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Abstract. Analyses of microbial communities from six water-flooding petroleum reservoirs at temperatures from 21 to 63 • C by 16S rRNA gene clone libraries indicates the presence of physiologically diverse and temperature-dependent microorganisms in these subterrestrial ecosystems. In samples originating from high-temperature petroleum reservoirs, most of the archaeal sequences belong to thermophiles affiliated with members of the genera Thermococcus, Methanothermobacter and the order Thermoplasmatales, whereas bacterial sequences predominantly belong to the phyla Firmicutes, Thermotogae and Thermodesulfobacteria. In contrast to high-temperature petroleum reservoirs, microorganisms belonging to the Proteobacteria, Methanobacteriales and Methanomicrobiales were the most encountered in samples collected from low-temperature petroleum reservoirs. Canonical correspondence analysis (CCA) revealed that temperature, mineralization, ionic type as well as volatile fatty acids showed correlation with the microbial community structures, in particular members of the Firmicutes and the genus Methanothermobacter showed positive correlation with temperature and the concentration of acetate. Overall, these data indicate the large occurrence of hydrogenotrophic methanogens in petroleum reservoirs and imply that acetate metabolism via syntrophic oxidation may represent the main methanogenic pathway in high-temperature petroleum reservoirs.

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Section: Discussionmentioning

confidence: 99%

Molecular analysis of the microbial community structures in water-flooding petroleum reservoirs with different temperatures

et al. 2012

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“…The species detected were putative anaerobes closely related to Clostridium and Alkaliphilus (Fig. 4b) and were similar to other clones from gas hydrates, oil wells, and anaerobic hydrocarbon enrichments (53,75).…”

Section: Vol 74 2008 Quantifying Microbial Utilization Of Hydrocarbmentioning

confidence: 95%

Quantifying Microbial Utilization of Petroleum Hydrocarbons in Salt Marsh Sediments by Using the ¹³ C Content of Bacterial rRNA

Pearson

Kraunz

Sessions

et al. 2008

Appl Environ Microbiol

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Natural remediation of oil spills is catalyzed by complex microbial consortia. Here we took a wholecommunity approach to investigate bacterial incorporation of petroleum hydrocarbons from a simulated oil spill. We utilized the natural difference in carbon isotopic abundance between a salt marsh ecosystem supported by the 13 C-enriched C 4 grass Spartina alterniflora and 13 C-depleted petroleum to monitor changes in the 13 C content of biomass. Magnetic bead capture methods for selective recovery of bacterial RNA were used to monitor the 13 C content of bacterial biomass during a 2-week experiment. The data show that by the end of the experiment, up to 26% of bacterial biomass was derived from consumption of the freshly spilled oil. The results contrast with the inertness of a nearby relict spill, which occurred in 1969 in West Falmouth, MA. Sequences of 16S rRNA genes from our experimental samples also were consistent with previous reports suggesting the importance of Gamma-and Deltaproteobacteria and Firmicutes in the remineralization of hydrocarbons. The magnetic bead capture approach makes it possible to quantify uptake of petroleum hydrocarbons by microbes in situ. Although employed here at the domain level, RNA capture procedures can be highly specific. The same strategy could be used with genus-level specificity, something which is not currently possible using the 13 C content of biomarker lipids.

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“…The LAI-59 OTU sequence showed maximum similarity of 95.7% to the 16S rRNA gene of uncultured soil bacterium (DQ378233), retrieved from oil polluted soil. The clones LAI-1 and LAI-27 were distantly related to Fusibacter paucivorans (AF050099), an anaerobic, thiosulfate reducing firmicute [30] and Tindallia magadiensis (Y15626), an anaerobic ammonifier isolated from soda lake deposits [31], respectively. Although, it is not possible to deduce the physiological attributes of an organism from the phylogenetic position of its 16S rRNA gene when the similarities to its nearest cultivated neighbor are distant, some general features of elements of the food web known to occur in mats can be predicted.…”

Section: Rest Of the Three Otus (Lai-59 Lai-27 And Lai-1)mentioning

confidence: 99%

Temporal bacterial diversity and detection of putative methanotrophs in surface mats of Lonar crater lake

Surakasi¹,

Antony²,

Sharma³

et al. 2010

J. Basic Microbiol.

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The phylogenetic diversity of bacterial communities in microbial mats of two different seasons from saline and hyperalkaline Lonar Lake was investigated using 16S rRNA gene library analysis. Arthrospira (Cyanobacteria) related clones (>80% of total clones) dominated libraries of both the seasons. Clear differences were found in both the seasons as the operational taxonomic units (OTUs) related to Fusibacter (LAI-1 and LAI-59) and Tindallia magadiensis found in post-monsoon were not found in the pre-monsoon library. Likewise, OTUs related to Planococcus rifietensis (LAII-67), Bordetella hinzii (LAII-2) and Methylobacterium variabile found in the pre-monsoon were not found in post-monsoon. The study was extended to identify methanotrophs in the surface mats. Libraries constructed with type I and type II methanotroph specific 16S rRNA gene primers showed the presence of clones (LAMI-99 and LAMII-2) closely related to Methylomicrobium buryaticum and Beijerinckiaceae family members. Denaturing gradient gel electrophoresis (DGGE) fingerprinting based on protein-coding genes (pmoA and mxaF) further confirmed the detection of Methylomicrobium sp. Hence, we report here for the first time the detection of putative methanotrophs in surface mats of Lonar Lake. The finding of clones related to organisms with interesting functional attributes such as assimilation of C 1 compounds (LAII-25, LAMI-39, LAMI-99 and LAMII-2), non-sulfur photosynthetic bacteria (LAMII-43) and clones distantly affiliated to organisms of heavily polluted environments (LAI-59 and LAMII-52), is of significant note. These preliminary results would direct future studies on the functional dynamics of microbial mat associated food web chain in the extreme environment.

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Fusibacter paucivorans gen. nov., sp. nov., an anaerobic, thiosulfate-reducing bacterium from an oil-producing well

Cited by 142 publications

References 27 publications

Molecular analysis of the microbial community structures in water-flooding petroleum reservoirs with different temperatures

Molecular analysis of the microbial community structures in water-flooding petroleum reservoirs with different temperatures

Quantifying Microbial Utilization of Petroleum Hydrocarbons in Salt Marsh Sediments by Using the ¹³ C Content of Bacterial rRNA

Temporal bacterial diversity and detection of putative methanotrophs in surface mats of Lonar crater lake

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Fusibacter paucivorans gen. nov., sp. nov., an anaerobic, thiosulfate-reducing bacterium from an oil-producing well

Cited by 142 publications

References 27 publications

Molecular analysis of the microbial community structures in water-flooding petroleum reservoirs with different temperatures

Molecular analysis of the microbial community structures in water-flooding petroleum reservoirs with different temperatures

Quantifying Microbial Utilization of Petroleum Hydrocarbons in Salt Marsh Sediments by Using the 13 C Content of Bacterial rRNA

Temporal bacterial diversity and detection of putative methanotrophs in surface mats of Lonar crater lake

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Quantifying Microbial Utilization of Petroleum Hydrocarbons in Salt Marsh Sediments by Using the ¹³ C Content of Bacterial rRNA