FusariumSpecies in Agriculture Industry and Control of Them

Chehri, Khosrow

doi:10.19080/artoaj.2017.06.555681

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…proliferatum are the predominant species that infect maize, rice, and wheat, etc., and cause vascular wilt, head blight, root rot, stem canker and plant death ( Lorenzo et al, 2012 ; Pasquali et al, 2016 ; Gabriela et al, 2018 ;). Fusarium verticillioides is a dominant species associated with maize in India ( Mohammed et al, 2016 ), widely spread throughout the world, thus affecting the yield, nutritional value, and quality of the grains ( Khosrow, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India

2023

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In the present investigation, prevalence, genetic diversity, and mycotoxin producing potential of Fusarium species associated with maize grain samples were studied from different geographical regions of India. The highest prevalence of Fusarium verticillioides was recorded as 88.52%, followed by F . coffeatum , F . foetens , and F . euwallaceae, 6.55%, 3.27%, and 1.63%, respectively. We isolated 54 strains of F . verticillioides , and their genetic diversity was studied by inter simple sequence repeats (ISSR). The ISSR fingerprints (AG) 8C and (AG) 8G showed 252 and 368 microsatellite sites in the genome of F . verticillioides and resulted in 99-100% repeatability and reproducibility. The Simpson (SID) and Shannon (H) indices (0.78 and 2.36) suggest that F . verticillioides strains exhibit moderate to high diversity. Molecular detection of fumonisin B1 (FB1) biosynthetic genes ( FUM1 and FUM13 ) involved in FB1 production in F . verticillioides was confirmed by polymerase chain reaction (PCR). Furthermore, 91% of the strains were positive for FB1 production, which was affirmed by liquid chromatography with tandem mass spectrometry (LC-MS-MS). In-vitro appurtenance of F . verticillioides spores exhibited a high to moderate effect on the growth and development of the maize. The current finding demonstrated that most F . verticillioides strains showed a wide range of genetic diversity with varied toxigenic and pathogenic potentials. In conclusion, for the first time, F . coffeatum , F . foetens , and F . euwallaceae species were reported from maize grain samples in India. They were positive for FB1 and negatively affecting grain quality, which is a major concern in food safety.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India

2023

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“…Food and agriculture organization (FAO) predicted that by 2030 the overall demand for maize will increase to around 300 million tons [5]. Fusarium is a ubiquitous plant pathogenic fungus that endanger plant growth and consistently adulterate economically important agriculture produce [6,7]. F. graminearum, F. culmorum, F. avenaceum, F. poae, F. sporotrichioides, F. equiseti, F. verticillioides, and F. proliferatum are the predominant species that infect maize, rice, wheat etc., and cause vascular wilt, head blight, root rot, stem canker and plant death [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Toxigenic Fusarium Verticillioidesassociated with Maize Grains in India

Navale

Vamkudoth

2021

Preprint

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Genetic diversity of Fusarium species associated with maize grains were analyzed from ten states of India. Fusarium verticillioides (88.52%), F. coffeatum (6.55%), F. foetens (3.27%) and F. euwallaceae (1.63%) were recovered from the maize samples. A phylogenetic analysis was performed based on the translation elongation factor 1 alpha (TEF1-α) gene sequence formed five evolutionary lineages (EL) that showed both inter- and intra-species variability. A In-vitro pathogenicity study confirms that among the 61 strains, 51% exhibited high to moderate virulence to the maize, and produced one or more mycotoxins. The genetic diversity of F. verticillioides were evaluated using Inter simple sequence repeats (ISSR) fingerprints. The ISSR fingerprints (AG)8C and (AG)8G shown 252 and 368 microsatellites sites, respectively, in the genome of F. verticillioides. The (AG)8C fingerprints differentiated the pathogenic and non-pathogenic clustering of F. verticillioides. However, Simpson’s (SID), and Shannon’s (H) index showed a wide range of diversity within the F. verticillioides. The pleomorphic nature of these strains role in host pathogen interactions and adoptive potential to develop disease to crop plants. Toxigenic potential of F. verticillioides was evaluated for the presence of FUM1and FUM13 genes involved in fumonisins B1 (FB1) biosynthesis, and further confirmed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) showed 91% strains were FB1 producers. The current finding demonstrated that most F. vertcillioides strains showed a wide range of genetic diversity with varied toxigenic and pathogenic potentials. Thus, there is an urgent need to develop an effective resistant breeding program in maize, and further monitoring is necessary for the post-harvest losses of grains.

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FusariumSpecies in Agriculture Industry and Control of Them

Cited by 2 publications

References 19 publications

Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India

Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India

Genetic Diversity of Toxigenic Fusarium Verticillioidesassociated with Maize Grains in India

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