Fusarium oxysporum KB-3 from Bletilla striata: an orchid mycorrhizal fungus

Jiang, Jian-wei; Zhuang, Ke; Cheng, Sheng; Nie, Qian-wen; Zhou, Shen-Xian; Chen, Qingqing; Zhou, Jinglong; Zhang, Xiao; Li, Xue Ting; Zhen, Tong Wen; Xu, Ming-Yue; Hsiang, Tom; Sun, Zhengxiang; Zhou, Yi

doi:10.1007/s00572-019-00904-3

Cited by 64 publications

(59 citation statements)

References 40 publications

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“…Fusarium spp. has been reported to cause root rot in commercial orchid species such as vanilla [53], and it is also described as mycorrhizal fungi in Bletilla striata [54]. Similarly, some saprotrophic fungi that we detected in this study, such as Aspergillus, Penicillium, Gymnopilus, Pyrenochaeta, Trichoderma and Neocosmospora, are found to form orchid mycorrhizal [55,56].…”

Section: The Functional Roles Of Root-associated Fungal Endophytessupporting

confidence: 70%

Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)

Zeng

Diao

et al. 2021

JoF

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Orchids of the genus Bletilla are well-known ornamental plants and sources of traditional medicine in Asia that rely on the symbiotic relationship with root endophytic fungi throughout their whole life cycle. However, little is known about their fungal partners, infection pattern, and pathways of carbon gain. We investigated carbon and nitrogen stable isotope patterns in different organs of three Bletilla species, identified the root endophytic fungal community composition, and determined mycorrhizal colonization rates. The three Bletilla species were comprised by a polyphyletic group which belongs to different trophic modes, such as saprotroph, pathotroph, and symbiotroph; however, the dominant species and their abundances varied among Bletilla spp. Mycorrhizal infection rates also varied among Bletilla species, with B. striata (65% ± 25%) being significantly higher than those of B. formosana (35% ± 16%) and B. ochracea (22% ± 13%). Compared with surrounding autotrophic plants, all Bletilla spp. were significantly enriched in 13C with B. striata to a significantly higher level than other two Bletilla species. Among different organs, stems had higher δ13C values, while leaves and flowers had higher δ15N and total N content values across all three species. Our results indicate that the symbiotic relationship of Bletilla and its root endophytic fungi is not strictly specific. Although mycorrhizal infection rates were highly variable, the three Bletilla species had the same infection pattern with hyphae penetrating the cortex cell by the pathway cell. Different Bletilla species have different strategies for C allocation among plant organs. These findings provide new insights into the ecological adaptation of orchids and will contribute to Bletilla germplasm conservation and sustainable utilization.

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Section: The Functional Roles Of Root-associated Fungal Endophytessupporting

confidence: 70%

Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)

Zeng

Diao

et al. 2021

JoF

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“…(Bayman et al 1997, Tremblay 2008, Yuan et al 2009, Sudheep & Sridhar 2012 and Fusarium spp. (Bayman et al 1997, Behera, Tayung & Mohapatra 2013, Jiang et al 2019, Yuan et al 2009 have been isolated from the roots of epiphytic orchids and have demonstrated growth-promoting effects on their hosts (Jiang et al 2019, Ovando et al 2005. Likewise, similar effects have been reported for Meyerozyma sp.…”

Section: Discussionmentioning

confidence: 99%

Endophytic fungi associated with roots of epiphytic orchids in two Andean forests in Southern Ecuador and their role in germination

et al. 2020

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Orchids are known to establish complex relationships with endophytic fungi throughout their life cycle, and particularly during germination of their reserves-deprived seeds. Characterizing generalist or specialist interactions between orchids and associated fungi is key to supporting orchid conservation efforts. Here, endophytic fungi associated with roots of epiphytic orchids were studied in two montane Andean forests in Southern Ecuador. Orchid root samples were collected from ten ~500 m2 plots distributed between two neighboring forests. Endophytic fungi associated with these roots were then isolated, cultured, and identified by sequencing of rDNA markers. In total, 52 pure isolates were recovered from the roots of 10 orchid species. These isolates were classified into nine taxonomic groups except for one isolate that remained unclassified. Most fungal isolates were found in roots of up to two different orchid species; however, Coprinellus radians was found in the roots of all sampled orchids. The potential of C. radians to promote germination of orchid seeds was tested in a separate assay using seeds from two orchid species different than those found in the experimental forest plots. Of the two C. radians isolates tested, one improved germination in the two orchids evaluated to a level about half of that observed in seeds germinated in nutrient-rich medium (Phytamax) and above the null germination observed in plates without the fungus. Together, these results revealed a generalist relationship between C. radians and all the studied epiphytic orchids and the potential role of this fungus as a promoter of orchid seed germination. Key Words: Agaricales, Epidendrum, symbiotic germination

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“…Interestingly, although Fusarium species have been reported as pathogens in many orchid species [39], especially F. oxysporum, which was reported to cause wilt disease in D. o cinale [40], other studies have suggested that Fusarium species can stimulate seed germination in the terrestrial orchid Cypripedium reginae [41] and enhance resistance to pathogens and promote plant growth in Dendrobium species [42,43]. In a recent study, F. oxysporum KB-3, obtained from the roots of Bletilla striata, was considered an OMF because it could promote seed germination of B. striata, establish colonization and produce coiled hyphal structures within the cortical cells in the roots of B. striata and Dendrobium candidum [44]. As noted in many studies, the border between endophytic and mycorrhizal fungi could be di cult to de ne, and some fungus-plant interactions can easily shift from mutualism to parasitism depending on the plant's physiology and environmental conditions [45,46,47].…”

Section: Discussionmentioning

confidence: 99%

In Situ Seedling Baiting to Isolate Plant Growth-Promoting Fungi From Dendrobium Officinale, An Over-Collected Medicinal Orchid In China

Chen

Wang

et al. 2021

Preprint

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Background: Orchids are highly dependent on mycorrhizae for survival and growth. Traditionally, to obtain orchid mycorrhizal fungi (OMFs) for plant growth, fungi have been isolated from the roots of mature wild plants; however, the species of OMF may change as the plants undergo different developmental stages. In this study, we developed the idea of using in situ seedling baiting to capture seedling growth-promoting fungi from Dendrobium officinale, an overcollected medicinal orchid in China.Results: In vitro-produced seedlings of D. officinale were transplanted into their original habitats, and newly established roots of well-growing seedlings were sampled for fungal isolation. Roots were sampled at 6 different times over one year, and five Tulasnella species and one Fusarium species were obtained and identified morphologically and molecularly. The ability to promote seedling growth was tested in three Tulasnella species TPYD-1, TPYD-2 and TPYD-3, with high isolation frequencies by inoculation onto in vitro-produced D. officinale seedlings. Although the three fungi were closely related species and clustered together in the phylogenetic tree, they showed different efficiencies in promoting D. officinale seedling growth. Tulasnella TPYD-2 showed a strong ability to promote seedling growth and could be selected for use in restoration plantings of D. officinale.Conclusions: Our results suggest that using an in situ seedling baiting technique could be an efficient way to obtain seedling growth-promoting fungi, and this approach could have broad applications in orchid mycorrhiza studies and orchid conservation.

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Fusarium oxysporum KB-3 from Bletilla striata: an orchid mycorrhizal fungus

Cited by 64 publications

References 40 publications

Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)

Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)

Endophytic fungi associated with roots of epiphytic orchids in two Andean forests in Southern Ecuador and their role in germination

In Situ Seedling Baiting to Isolate Plant Growth-Promoting Fungi From Dendrobium Officinale, An Over-Collected Medicinal Orchid In China

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