Fusarium circinatum and pitch canker of Pinus in Colombia

Steenkamp, Emma Theodora; Rodas, Carlos A.; Kvas, Marija; Wingfield, Michael J.

doi:10.1007/s13313-012-0120-z

Cited by 39 publications

(41 citation statements)

References 41 publications

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“…Isolates were examined microscopically to allow for morphology-based identification of F. circinatum (Nirenberg and O'Donnell 1889). The identity of presumptive F. circinatum isolates were then confirmed using PCR-based species-specific diagnosis as previously described (Steenkamp et al 2012). …”

Section: Isolation Of Fusarium Circinatummentioning

confidence: 99%

Re-use of seedling containers andFusarium circinatumassociation with asymptomaticPinus patulaplanting stock

Morris¹,

Fourie²,

Greyling³

et al. 2014

Southern Forests: a Journal of Forest Science

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Fusarium circinatum is a pathogen causing serious post-planting mortality of Pinus patula seedlings in southern Africa. Containerized planting stock that is asymptomatic but associated with F. circinatum in the nursery is thought to be the cause of this problem. The aim of this study was to determine if re-use of seedling containers could be a source of inoculum resulting in asymptomatic planting stock and increased post-planting mortality of Pinus patula. Two experiments were conducted in successive years comparing nursery cull of symptomatic seedlings, seedling growth, association of F. circinatum with asymptomatic seedlings and post-planting mortality for crops raised in re-used containers, with and without sanitation, and factory new containers. Each experiment consisted of a nursery production trial followed by out-planting into pots to assess post-planting mortality. Our results show that re-use of containers without sanitation increases the cull of symptomatic seedlings, incidence of F. circinatum associated with asymptomatic seedlings and post-planting mortality compared to the re-use of containers after steam sanitation or factory new containers. Growth of asymptomatic seedlings was unaffected by container treatment or association with F. circinatum and in the absence of wilt symptoms the root system did not exhibit typical discolouration. Watering frequency did not influence post-planting mortality in pots. The comparison of two open-pollinated seed mixes of P. patula that, based on seedling stem inoculation screening, represented susceptible and tolerant material did not show differences in nursery cull or post-planting mortality. This work demonstrated that natural contamination of re-used containers can be a primary source of inoculum producing asymptomatic seedlings associated with F. circinatum that will succumb to the pathogen after field planting. The process of seedling infection, apparent latent infection in the seedling and expression of disease after planting needs greater understanding to improve nursery hygiene measures to control this disease.

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Section: Isolation Of Fusarium Circinatummentioning

confidence: 99%

Re-use of seedling containers andFusarium circinatumassociation with asymptomaticPinus patulaplanting stock

Morris¹,

Fourie²,

Greyling³

et al. 2014

Southern Forests: a Journal of Forest Science

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“…The pathogen was first detected in 1945 in Pinus virginiana Mill in the southeastern United States (Hepting & Roth, 1946). Since then, it has continued to expand throughout the world and has been found infecting different conifer species in Mexico (GuerraSantos, 1998), Haiti (Hepting & Roth, 1953), Japan (Kobayashi & Muramoto, 1989), South Africa (Viljoen et al, 1994, Coutinho et al, 2007, Chile (Wingfield et al, 2002), Korea (Cho & Shin, 2004), Uruguay (Alonso & Bettucci, 2009), Colombia (Steenkamp et al, 2012) and, more recently, in Brazil (Pfenning et al, 2014). In Europe, the pathogen has been also recorded in Spain (Dwinell, 1999;Landeras et al, 2005) (EPPO, 2006), Portugal (Bragança et al, 2009) and Italy (Carlucci et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Short communication: Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum

et al. 2018

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Aim of the study: To assess the impact on two mycoviruses recently described in F. circinatum mitovirus 1 and 2-2 (FcMV1 and FcMV2-2) on i) mycelial growth, ii) spore germination and iii) relative necrosis.Material and methods: Fourteen monosporic strains of F. circinatum (one of each pair infected with mycoviruses and the other without them) of the pathogen with and without viruses were selected for the assay. The statistical analysis, were a linear mixed model of analysis of variance considering one between-subjects factor (isolate) and one within-subjects factor with four levels (1=without viruses, 2=only virus FcMV1, 3=only virus FcMV2-2 and 4=both viruses).Main results: Colony growth rates of F. circinatum isolates were significantly reduced in presence of mycoviruses (p=0.002). The spore germination was also reduced in the F. circinatum isolates containing mycovirus as compared to mycovirus-free isolates (p<0.001). No significant differences in lesion lengths caused by F. circinatum were found in relation to the presence/absence of mycovirus (p<0.61).Research highlights: Reduction of the percentage of spore germination in the isolates of F. circinatum with mycovirus infections, as compared to free isolates, provides indications of reduction of metabolic activity and plant physiology are discussed. The lack of significant differences found in the length of the lesions caused by F. circinatum with respect to the presence/absence of mycovirus, indicates that further studies with a larger number of variables are required Additional keywords: pine pitch canker; hypovirulence; biological control; forest pathology.

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“…pini (Correll et al, 1991). The pathogen was then recorded in numerous countries worldwide, including Mexico, Colombia, Chile and Uruguay (Britz et al, 2001;Wingfield et al, 2002;Alonso & Bettucci, 2009;Steenkamp et al, 2012). F. circinatum is known to infect more than 50 species of pines (Wingfield et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

First report and characterization of Fusarium circinatum, the causal agent of pitch canker in Brazil

Pfenning¹,

Costa²,

Melo³

et al. 2014

Trop. plant pathol.

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Pitch canker is one of the most important diseases of pine trees worldwide, including South America. The causal agent of this disease is Fusarium circinatum, a member of the Gibberella fujikuroi complex (GFC). In South America, the species is reported from Colombia, Uruguay and Chile, but is considered a quarantine organism in Brazil. In this study we characterized isolates obtained from symptomatic pine seedlings intercepted in a nursery in Santa Catarina State using phylogenetic analyses, crossings and morphological markers. The Brazilian isolates grouped with reference material in an unique clade and formed fertile perithecia when crossed with reference tester strains. The detailed characterization given here and the availability of tester strains will allow for reliable identification and support monitoring of this important plant pathogen.

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Fusarium circinatum and pitch canker of Pinus in Colombia

Cited by 39 publications

References 41 publications

Re-use of seedling containers andFusarium circinatumassociation with asymptomaticPinus patulaplanting stock

Re-use of seedling containers andFusarium circinatumassociation with asymptomaticPinus patulaplanting stock

Short communication: Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum

First report and characterization of Fusarium circinatum, the causal agent of pitch canker in Brazil

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