Further Studies on the Plasmodium berghei-Anopheles stephensi: Rodent System of Mammalian Malaria

Vanderberg, Jerome P.; Nussenzweig, Ruth S.; Most, Harry

doi:10.2307/3277136

Cited by 87 publications

(38 citation statements)

References 10 publications

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“…Immunizations P. berghei (NK65 strain) was maintained as described (22). Anesthetized mice were subjected to the bites of gamma-irradiated (15,000 rad; 1 rad ϭ 0.01 Gy) malaria-infected Anopheles stephensi mosquitoes for 10 min/day for 4 (BALB/c background, CD1-deficient mice) or 8 (C57BL/6 background, CD1-deficient mice) days, rested for 10 days, and boosted for 4 more days.…”

Section: Micementioning

confidence: 99%

Cutting Edge: The IgG Response to the Circumsporozoite Protein Is MHC Class II-Dependent and CD1d-Independent: Exploring the Role of GPIs in NK T Cell Activation and Antimalarial Responses

Molano

Park

Chiu

et al. 2000

The Journal of Immunology

120

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Biochemical analysis has suggested that self GPI anchors are the main natural ligand associated with mouse CD1d molecules. A recent study reported that Vα14+ NK T cells responded to self as well as foreign (parasite-derived) GPIs in a CD1d-dependent manner. It further reported that the IgG response to the Plasmodium berghei malarial circumsporozoite (CS) protein was severely impaired in CD1d-deficient mice, leading to a model whereby NK T cells, upon recognition of CD1d molecules presenting the CS-derived GPI anchor, provide help for B cells secreting anti-CS Abs. We tested this model by comparing the anti-CS Ab responses of wild-type, CD1d-deficient, and MHC class II-deficient mice. We found that the IgG response to the CS protein was solely MHC class II-dependent. Furthermore, by measuring the response of a broad panel of CD1d-autoreactive T cells to GPI-deficient CD1d-expressing cells, we found that GPIs were not required for autoreactive responses.

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Section: Micementioning

confidence: 99%

Cutting Edge: The IgG Response to the Circumsporozoite Protein Is MHC Class II-Dependent and CD1d-Independent: Exploring the Role of GPIs in NK T Cell Activation and Antimalarial Responses

Molano

Park

Chiu

et al. 2000

The Journal of Immunology

120

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“…Sporozoites of P. vivax and P. falciparum (isolates from Thailand), as well as P. knowlesi (H strain), were obtained from mosquito salivary glands 14-17 d after an infective blood meal (8). Sporozoite extracts were prepared by placing a known number of sporozoites in 500/~l of phosphate-buffered saline containing i% bovine serum albumin (PBS-BSA).…”

Section: A T E R I a L S A N D M E T H O D Smentioning

confidence: 99%

Circumsporozoite proteins of malaria parasites contain a single immunodominant region with two or more identical epitopes.

Zavala

Cochrane

Nardin

et al. 1983

The Journal of Experimental Medicine

296

151

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We have used panels of monoclonal antibodies to circumsporozoite (CS) proteins of Plasmodium falciparium, P. vivax, and P. knowlesi to determine the number of topographically independent epitopes of these antigens. The results of competition binding assays indicated that single regions of the CS molecules were recognized by the homologous monoclonal antibodies. Competition binding assays were also used to study the specificity of antibodies contained in the sera of humans and monkeys that had developed sterile immunity after immunization with irradiated, intact sporozoites. We found that single monoclonal antibodies inhibited 70-95% of the specific binding of the polyclonal antibodies to crude extracts of sporozoites. It appears, therefore, that CS proteins are among the most immunogenic constituents of sporozoites, and that a single region of these molecules contains most of the immunogenic activity. An additional finding was that the immunodominant region of CS molecules is multivalent with regard to the expression of a single epitope. This was demonstrated by the ability of monomers of CS proteins to bind simultaneously two or more molecules of the same monoclonal antibody.

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“…The NK 65 strain of P. berghei was used in all experiments. This strain has been maintained in hamsters by passage with malaria-infected blood, alternating with mos(luito-induced infections (7). Mice were injected intravenously with 104 infected erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

Complement-mediated Defect in Clearance and Sequestration of Sensitized, Autologous Erythrocytes in Rodent Malaria

Pappas¹,

Nussenzweig²,

Nussenzweig³

et al. 1981

J. Clin. Invest.

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A B S T R A C T We investigated the ability of malariainfected and normal mice to clear particulate immune complexes consisting of autologous erythrocytes sensitized with either IgG or complement.Normal mice rapidly clear autologous erythrocytes optimally sensitized with IgG and the liver plays a major role in their sequestration. Clearance of optimally sensitized erythrocytes is complement-dependent because cobra venom factor-treated, normal mice failed to clear these cells rapidly, unless they had been pretreated with fresh mouse serum.In the initial phase ofPlasmodium berghei infection, clearance of the optimally sensitized erythrocytes was markedly increased over that observed in normal mice. 2 wk after infection, however, clearance was minimal. This defect was most likely the consequence of the hypocomplementemia observed at this stage of infection since sensitized erythrocytes were removed rapidly from the blood if they had been coated with C3 in vitro before injection into malarial mice.The functional activity of the complement receptors of phagocytic cells was assayed in malarial mice by the injection ofautologous erythrocytes coated with C3 and C4 in the absence ofantibody. The complement-coated erythrocytes were rapidly removed from the blood, accumulated in the liver, and then gradually returned This study is partial fulfillment of the requirements for the

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Further Studies on the Plasmodium berghei-Anopheles stephensi: Rodent System of Mammalian Malaria

Cited by 87 publications

References 10 publications

Cutting Edge: The IgG Response to the Circumsporozoite Protein Is MHC Class II-Dependent and CD1d-Independent: Exploring the Role of GPIs in NK T Cell Activation and Antimalarial Responses

Cutting Edge: The IgG Response to the Circumsporozoite Protein Is MHC Class II-Dependent and CD1d-Independent: Exploring the Role of GPIs in NK T Cell Activation and Antimalarial Responses

Circumsporozoite proteins of malaria parasites contain a single immunodominant region with two or more identical epitopes.

Complement-mediated Defect in Clearance and Sequestration of Sensitized, Autologous Erythrocytes in Rodent Malaria

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