Further Studies on the Nuclear Chromatin of Morphologically Abnormal Bull Spermatozoa

Gledhill, Barton L.

doi:10.1530/jrf.0.0290431

Cited by 11 publications

(8 citation statements)

References 2 publications

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“…This increased thickness may have affected (Gledhill et al, 1971). This was subsequently related to structural defects in the chromatin (Gledhill, 1972). However, the discrepancy may have resulted from determining dye binding on intact bull spermatozoa.…”

Section: Resultsmentioning

confidence: 99%

Quantitative fluorometry of abnormal mouse sperm nuclei

Pogany¹,

Balhorn²

1992

Reproduction

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Summary. An extensive quantitative analysis of deformed mouse spermatozoa was undertaken. Improvements over previous studies included the isolation and purification of sperm nuclei, a multifaceted analytical approach using several fluorochromes and the analysis of individual nuclei classified into shape categories.Malformed sperm nuclei in BALB/c mice could not be distinguished from normal ones in terms of total and basic proteins, sulfhydryl and disulfide group concentration, DNA concentration and chromatin organization. The shape of sperm nuclei is therefore probably determined by the manner in which the internal biochemical components are assembled.

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Section: Resultsmentioning

confidence: 99%

Quantitative fluorometry of abnormal mouse sperm nuclei

Pogany¹,

Balhorn²

1992

Reproduction

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“…Gledhill (1966) foi o primeiro a identificar espermatozoides com cromatina malcompactada em touros subférteis, utilizando reação de Feulgen. Outro método empregado para avaliação da cromatina foi desenvolvido por Mello (1982), o qual emprega o azul de toluidina, um corante catiônico que apresenta metacromasia.…”

Section: Introductionunclassified

Avaliação computacional da compactação da cromatina e de características morfométricas da cabeça de espermatozoides de coelho (Oryctolagus cuniculus)

Kanayama

Beletti

2011

Arq. Bras. Med. Vet. Zootec.

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RESUMOInvestigou-se a correlação entre a morfometria da cabeça e a intensidade da condensação e heterogeneidade da cromatina em espermatozoides de coelho (Oryctolagus cuniculus). Para tal, utilizaram-se 35 esfregaços de sêmen de coelhos da raça Nova Zelândia, corados com azul de toluidina e avaliados por análise de imagem computacional. As imagens foram obtidas digitalmente em tons de cinza e avaliadas por algoritmos desenvolvidos em ambiente de programação Scilab. As mensurações obtidas da cabeça dos espermatozoides foram área, perímetro, comprimento, largura, relação comprimento largura, elipsidade, fator de forma, descritores Fourier e simetria lateral e anteroposterior. Também foram avaliadas a intensidade da compactação e a heterogeneidade da cromatina espermática. Os espermatozoides de coelho apresentaram compactação e heterogeneidade cromatínica mais intensas do que os de touro e observou-se correlação significativa entre características morfométricas da cabeça e compactação e heterogeneidade cromatínica. Conclui-se que a cromatina é importante para a constituição morfológica da cabeça de espermatozoides de coelho e que a cromatina espermática de coelho é naturalmente mais heterogênea e menos compactada que a de touro.Palavras-chave: coelho, análise de imagem, morfometria espermática, alterações de cromatina ABSTRACT The correlation between the head morphometry and the intensity of condensation and heterogeneity of sperm chromatin were investigated in rabbit (Oryctolagus cuniculus). To this, 35 semen smears from New

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“…In the present study, ethidium bromide (EB), which is a specific fluorochrome for DNA, has been used to visualize the extent of chromatin decondensation occurring during sperm maturation. The uptake of DNA-specific dyes by the spermatozoa reflects the maturation of the DNA-DNP complex (Gledhill, 1972). The EB fluorescence emitted from the spermatozoa has been correlated with the maturation of the DNA-DNP complex of the spermatozoa (Johannisson and Jacolino, 1987).…”

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confidence: 99%

Sperm maturation in rhesus monkey: Changes in ultrastructure, chromatin condensation, and organization of lipid bilayer

Sivashanmugam

Rajalakshmi

1997

Anat. Rec.

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Background The changes in ultrastructure, lipid organization, chromatin decondensation, and denaturation of rhesus monkey spermatozoa during epididymal maturation were studied. This study would provide background information that would be useful to evaluate adverse effects, if any, caused by the use of contraceptive agents. Methods Adult sexually mature rhesus monkeys were castrated under ketamine anesthesia. The epididymis was divided into initial segment, caput, corpus, and cauda epididymides. To study changes in lipid organization of the sperm plasma membrane during epididymal transit, spermatozoa from different epididymal segments and ejaculated spermatozoa were exposed to merocyanine 540 (MC 540). The changes in chromatin denaturation and decondensation were assessed by using the nucleic acid‐specific fluorochromes, acridine orange, and ethidium bromide, respectively, prior to and after exposure to dithiothreitol (DTT). Results Testicular spermatozoa (∼40%) showed localization of MC 540 mainly in the midpiece, whereas remaining sperm did not localize MC 540. Spermatozoa from the initial segment of the epididymis showed uniform distribution of MC 540 localization in the head and midpiece. A pattern of localization of MC 540 similar to mature caudal and ejaculated sperm in which the staining was restricted to the acrosome and the midpiece first appeared in a small percentage of caput spermatozoa and was completed during transit through the corpus epididymidis. Mature spermatozoa from cauda epididymidis, vas deferens, and ejaculate did not undergo chromatin denaturation even after exposure to 10 mM DTT, unlike sperm from testis, initial segment, and caput epididymidis. Spermatozoa exposed to DTT showed chromatin decondensation; maximum decondensation was seen in testicular sperm and a decrease in the percentage of sperm, showing decondensation, occurred during epididymal transit. Ultrastructural studies showed that spermatozoa undergo structural changes during sperm maturation. Conclusions The present study shows that rhesus monkey spermatozoa undergo reorganization of the plasma membrane lipids and stabilization of disulfide linkages during epididymal transit. The results would be of use in evaluating the action of potential male contraceptive drugs on epididymal spermatozoa. Anat. Rec. 247:25–32 © Wiley‐Liss, Inc.

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Further Studies on the Nuclear Chromatin of Morphologically Abnormal Bull Spermatozoa

Cited by 11 publications

References 2 publications

Quantitative fluorometry of abnormal mouse sperm nuclei

Quantitative fluorometry of abnormal mouse sperm nuclei

Avaliação computacional da compactação da cromatina e de características morfométricas da cabeça de espermatozoides de coelho (Oryctolagus cuniculus)

Sperm maturation in rhesus monkey: Changes in ultrastructure, chromatin condensation, and organization of lipid bilayer

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