Further studies on the exocellular lipase of Rhizopus arrhizus

Sémériva, Michel; Dufour, C.

doi:10.1016/0005-2760(72)90054-9

Cited by 40 publications

(9 citation statements)

References 13 publications

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“…Formation of PC-OO was not observed. This could be due to the inhibition of lipases by free fatty acids, which is a well-known phenomenon in emulsion systems (18). The effects of PC-MM and oleic acid concentrations on the extent of the transesterification reaction in toluene and in solvent-free reaction medium were compared.…”

Section: Resultsmentioning

confidence: 99%

Transesterification of phospholipids in different reaction conditions

Mustranta

Suortti

Poutanen

1994

J Americ Oil Chem Soc

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Transesterification of synthetic dimyristoyl phosphatidylcholine with oleic acid by commercial lipase preparations from AspergiUus niger and Rhizomucor miehei was studied in the presence and absence of solvent. A high-performance liquid chromatography method for determination of the modified phosphatidylcholine was developed. Under solvent-free conditions, transesteriflcation could be carried out as efficiently as in toluene, and the degree of hydrolysis was lower than in toluene. Transesteriflcation was influenced by the water content as well as by the fatty acid concentration in the reaction mixture. The optimum water content for transesterification in solvent-free reaction medium was higher than in toluene with both lipases. The yield of modified phosphatidylcholine increased, and the degree of hydrolysis decreased with increasing fatty acid concentration. The maximum yield of modified phosphatidyleholine, 35% of the original phospholipid, was obtained with R. miehei lipase.

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Section: Resultsmentioning

confidence: 99%

Transesterification of phospholipids in different reaction conditions

Mustranta

Suortti

Poutanen

1994

J Americ Oil Chem Soc

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“…Inhibition of Iipases by free acids in emulsion systems is a well known phenomenon. '6, 29 From Figure 9, one derives that retention of large concentrations of triglycerides is most efficient at high free fatty acid concentrations, and this effect is still enhanced by working at low relative humidity circumstances.…”

Section: Parameters Influencing Reaction Kineticsmentioning

confidence: 99%

Lipase‐catalyzed ester exchange reactions in organic media with controlled humidity

Goderis

Ampe

Feyten

et al. 1987

Biotech & Bioengineering

184

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Immobilized lipase activity is studied in organic solvent systems of controlled water content under the influence of a variety of reaction parameters, such as temperature, relative humidity, substrate concentrations, and type of fatty acid used. Control of the amount of water in the reaction system was found to be a valuable tool for the orientation of the reaction process and for the determination of the final reaction products. The properties of the immobilized lipase were studied using the interesterification of triolein and palmitic acid as the model system.

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“…Infants ingest large quantities of milk lipids, which are present in phospholipid-protein-stabilized droplets (17). In many cases their bile salt concentrations are low (18), but they digest the milk lipids rather efficiently (19 (21). Lipase and colipase were purified from pig pancreas according to Verger et al (22) and Erlanson and Borgstrom (23).…”

Section: Introductionmentioning

confidence: 99%