Further studies on the asymmetry of chromosome 15 duplication in trisomic leukemias of heterozygous origin: Preferential status of the AKR chromosome

Wirschubsky, Zvi; Wiener, Francis; Bregula, Ursula; Klein, George

doi:10.1002/ijc.2910340217

Cited by 13 publications

(11 citation statements)

References 19 publications

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“…14) In addition, a preferential duplication of chromosome 15 derived from the AKR mouse was demonstrated in T cell lymphomas in (AKR×B6)F 1 mice. 15) Ohno et al demonstrated that mineral oil-or pristane-induced plasmacytomas contain nonrandom chromosomal translocations, t(6;15) or t(12;15), 6,7) and Suematsu et al were able to generate monoclonal transplantable plasmacytomas with the chromosomal translocation t(12;15) in interleukin 6 transgenic mice of BALB/c background, but not in those of C57BL/6 background. 16,17) We also observed trisomy of chromosome 6 and/or 12 in lymphoma cells developed in the BALB/c background.…”

Section: Discussionmentioning

confidence: 99%

Strain‐dependency of Chromosomal Abnormalities in Lymphomas Developed in Eμ‐myc Transgenic Mice

Akagi

Yamamura

2001

Japanese Journal of Cancer Research

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Section: Discussionmentioning

confidence: 99%

Strain‐dependency of Chromosomal Abnormalities in Lymphomas Developed in Eμ‐myc Transgenic Mice

Akagi

Yamamura

2001

Japanese Journal of Cancer Research

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“…Metaphase chromosomes were prepared as previously described (12). Chromosomes were identified by comparison with the standard mouse karyotype (13).…”

Section: Methodsmentioning

confidence: 99%

“…The chromosomes were denatured by soaking the filters in 0.5 M NaOH/1.5 M NaCl, followed by neutralization in 0.5 M Tris HCl, pH 7.4/1.5 M NaCl and then 3 M NaCl/0.2 M NaH2PO4/0.02 M EDTA for 5 min each. After 120-min baking (80'C), all filters of the same experiment were hybridized (18) (25), 6 (26), 12 (26), and 15 (2,27,28), respectively. Karyotype.…”

Section: Methodsmentioning

confidence: 99%

“…We have therefore checked the chromosomal content of selected peaks by molecular hybridization of sorted chromosomes. The TKA7AA mouse lymphoma line (12) has been used for this purpose because it has an essentially normal karyotype with trisomy of chromosome 15, present in the form of one normal chromosome 15 and a Robertsonian 15;15 fusion chromosome (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

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Gene localization on sorted chromosomes: definitive evidence on the relative positioning of genes participating in the mouse plasmacytoma-associated typical translocation.

Wirschubsky¹,

Ingvarsson²,

Carstenssen³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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Experiments have been carried out to establish the relative position of the mouse Ig heavy chain locus and the c-myc oncogene. In mouse plasmacytoma with the typical rcpt(12;15) chromosome translocation the c-myc oncogene is juxtaposed to one of the heavy chain genes in a head-to-head orientation. Since the relative orientations of the c-myc locus and the Ig heavy chain gene cluster on the corresponding mouse chromosomes had not been settled, it was not known whether the rearranged c-myc gene is transposed to chromosome 12 or remains on chromosome 15. To decide which of the two alternatives is correct, we separated the translocation chromosomes by fluorescence-activated chromosome sorting. The separated chromosomal fractions were hybridized with mycspecific DNA probes corresponding to the ru-st or second/third exons in a chromosome spot assay. The results presented here indicate that the c-myc gene in mouse plasmacytoma carrying the typical translocation, as in the human Burkitt lymphoma analogous translocation, transposes to the chromosome carrying the Ig heavy chain locus. These results also establish the orientations of the Ig heavy chain locus and the c-myc locus on their normal chromosomes.The typical t(12;15) translocation in murine plasmacytoma (MPC) is a reciprocal exchange that leads to thejuxtaposition ofthe c-myc oncogene and Ig heavy chain (H) sequences. The

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“…The MCF-B line was derived from a thymic lymphoma induced in an AKR 6; 15 mouse by MCF-virus (Wirschubsky et al, 1984). This strain is a constitutional carrier of the Rb(6; 15) chr.…”

Section: Cell Linesmentioning

confidence: 99%

Mapping of the c-myc, pvt-1 and immunoglobulin kappa genes in relation to the mouse plasmacytoma-associated variant (6;15) translocation breakpoint.

Banerjee¹,

Wiener²,

Spira³

et al. 1985

The EMBO Journal

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A variant mouse plasmacytoma (MPC)‐associated translocation chromosome has arisen by pericentric inversion and exchange of the distal segments of a Robertsonian 6;15 fusion chromosome in the CAK TEPC 1198 mouse plasmacytoma, as described earlier. In situ hybridization was performed on the normal and the inverted Rb chromosomes, using myc and kappa probes. On the normal Rb chromosome, myc was in the 15 D2/3 region, whereas kappa hybridized in the 6 C2 area, as expected. On the inverted Rb chromosome, myc remains on the centrometric side of the translocation breakpoint on the chromosome 15‐derived portion, whereas kappa has moved to the chromosome 6‐derived segment that joined the same breakpoint on the telomeric side. Taken together with our recent demonstration that the murine c‐myc locus is oriented ‘head up’ on chromosome 15, and with the results of Cory and co‐workers concerning the relationship between the kappa gene and the associated pvt‐1 region in the CAK TEPC 1198 tumor, the following conclusions can be drawn: (i) in the variant translocation of the CAK TEPC 1198 MPC, the breakage occurs 3′ of the c‐myc gene, as in the human Burkitt lymphoma‐associated variant translocations; (ii) the pvt‐1 gene on chromosome 15 is distal to the myc gene; (iii) the kappa light chain locus is oriented ‘head up’ on mouse chromosome 6 and faces pvt‐1 and, beyond it, c‐myc, in a head‐to‐tail configuration.

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Further studies on the asymmetry of chromosome 15 duplication in trisomic leukemias of heterozygous origin: Preferential status of the AKR chromosome

Cited by 13 publications

References 19 publications

Strain‐dependency of Chromosomal Abnormalities in Lymphomas Developed in Eμ‐myc Transgenic Mice

Strain‐dependency of Chromosomal Abnormalities in Lymphomas Developed in Eμ‐myc Transgenic Mice

Gene localization on sorted chromosomes: definitive evidence on the relative positioning of genes participating in the mouse plasmacytoma-associated typical translocation.

Mapping of the c-myc, pvt-1 and immunoglobulin kappa genes in relation to the mouse plasmacytoma-associated variant (6;15) translocation breakpoint.

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