Further studies on hybrid cell-surface antigens associated with human chromosome 11

Abstract

A new human immunogenetic cell-surface activity associated with human chromosome 11 in the AL human-Chinese hamster ovary cell hybrid is described. Like a1, but not a2, it is present on the human erythrocyte. By mutagenesis and selection, specific, stable, variants of the AL hybrid have been prepared exhibiting various combinations of a1, a2, a3, and lactic dehydrogenase A activities. The antigens of the AL system can be demonstrated by the horseradish peroxidase system which offers a promising approach to scanning of tissue cells.

Assignment of the gene coding for the T3-delta subunit of the T3-T-cell receptor complex to the long arm of human chromosome 11 and to mouse chromosome 9.

“…These cell brid. The Southern blotting analysis with the cDNA insert of lines were characterized by cytogenetic and isozyme analypPGBC#9 as hybridization probe shows that the 14-kb Hinsis (27)(28)(29)(30)(31) and by DNA hybridization techniques (32,33). dIII T3-8-chain fragment is not detected in the DNA ob- tamned from this cell line (Fig.…”

Genetic fine-structure mapping in human chromosome 11 by use of repetitive DNA sequences.

“…11 as the AL hybrid (4,7,10 SAil-i, -2, and -3) have been shown to derive from loci.on chromosome 11, a, and a3 being on the-short and a2 on the long arm (5,10).…”

“…Previously, we isolated and characterized a human-Chinese hamster ovary, (CHO) hybrid cell containing only human chromosome 11.(4). We identified several genes coding for cell surface antigens carried by this chromosome (5)(6)(7)(8) and, using selection against.these markers, we derived a series of hybrid cells in which varying portions of the chromosome have been deleted (1, 2, .8). The isozyme markers lactate dehydrogenase A (LDHA) and acid phosphatase-2 (ACP2) were mapped to specific regions of the short arm of human chromosome 11 by using these hybrid cells (1).…”

Genetic and biochemical analysis of the a1 cell-surface antigen associated with human chromosome 11.

“…Methods to elucidate the genetic aspects of these molecules are needed to illuminate regulatory mechanisms. For these reasons we have developed methods, using the techniques of somatic cell genetics, to make possible in vitro analysis of human cell surface antigens by using human-Chinese hamster cell hybrids (1)(2)(3). The preferential loss of human chromosomes from these hybrids permits the preparation of stable hybrid lines containing only one human chromosome, therefore expressing only a portion of the entire spectrum of human cell surface antigens.…”

“…It has been shown that this system contains at least three antigenic components, al, a2, and a3 (2,3), and that some of these are present on cells of many tissues while others have a more limited distribution: al is present on human erythrocytes, (5,6). A method using the AL-1l hybrid for detection and quantitation of mutagenic actions has been described (7 15 ,tM thymidine, and 7.5% fetal calf serum macromolecular fraction.…”